AGRIVITA, Journal of Agricultural Science
Vol 35, No 1 (2013)

INFECTION PROCESS OF ENTOMOPATHOGENIC FUNGI Metarhizium anisopliae IN THE Tetranychus kanzawai (KISHIDA) (TETRANYCHIDAE: ACARINA)

Sanjaya, Yayan (Unknown)
Ocampo, Virginia R. (Unknown)
Caoili, Barbara L. (Unknown)



Article Info

Publish Date
27 Jun 2013

Abstract

The importance of fungal attack to acarina has been observed but not yet on Tetranychus kanzawai. When infected mite, either within in a same  species or even in a different  species of  entomopathigenic probably  have  similar signs and simptoms to mites.  Characteristic of entomopathogenic fungus to Tetranychus kanza-wai was investigated. Three selected isolates of Metarhizium anisopliae, from Philippines and Indonesia were evaluated. The following aspects were investigated: (1) Bioassay tes of virulence, (2) Koch Postulate and (3) Infection process on each fungus against mite. Virulence of  Metarhizium anisopliae was 1.4 X 103, 7.2 X 102 , 5 X 102 PIB per ml on Ma4, Ma5 and Ma6, respectively. On Koch Postulates, Reinoculation and reisolation of all seven isolates from infected T. kanzawai showed definite signs of infection. This demonstrated its entomopathogenic capacity. In this experiment, adult mites were sprayed with 108 per ml concentrations of conidia observed under light microscope and Scanning Electron Microscopy (SEM). The result found  T. kanzawai was very susceptible to three isolates M. anisopliae. In the end of the trials, fungal growth was detectable as early as 2 until 4 days of observation. Infection process with microphoto-graph and Scanning Electro Microscopy showed attachment, germination and penetration, extrusion and conidiogenesis fungal form. Keywords: Infection, Tetranychus kanzawai, Metarhizium anisopliae, Light micros-copy, Scanning Electro Microscopy (SEM)

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Journal Info

Abbrev

AGRIVITA

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

AGRIVITA Journal of Agricultural Science is a peer-reviewed, scientific journal published by Faculty of Agriculture Universitas Brawijaya Indonesia in collaboration with Indonesian Agronomy Association (PERAGI). The aims of the journal are to publish and disseminate high quality, original research ...