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All Journal AGRIVITA, Journal of Agricultural Science
Barbara L. Caoili
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Agriculture, Biological Sciences & Forestry

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INFECTION PROCESS OF ENTOMOPATHOGENIC FUNGI Metarhizium anisopliae IN THE Tetranychus kanzawai (KISHIDA) (TETRANYCHIDAE: ACARINA) Sanjaya, yayan; Ocampo, Virginia R.; Caoili, Barbara L.
AGRIVITA, Journal of Agricultural Science Vol 35, No 1 (2013)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The importance of fungal attack to acarina has been observed but not yet on Tetranychus kanzawai. When infected mite, either within in a same  species or even in a different  species of  entomopathigenic probably  have  similar signs and simptoms to mites.  Characteristic of entomopathogenic fungus to Tetranychus kanza-wai was investigated. Three selected isolates of Metarhizium anisopliae, from Philippines and Indonesia were evaluated. The following aspects were investigated: (1) Bioassay tes of virulence, (2) Koch Postulate and (3) Infection process on each fungus against mite. Virulence of  Metarhizium anisopliae was 1.4 X 103, 7.2 X 102 , 5 X 102  PIB per ml on Ma4, Ma5 and Ma6, respectively. On Koch Postulates, Reinoculation and reisolation of all seven isolates from infected T. kanzawai showed definite signs of infection. This demonstrated its entomopathogenic capacity. In this experiment, adult mites were sprayed with 108 per ml concentrations of conidia observed under light microscope and Scanning Electron Microscopy (SEM). The result found  T. kanzawai was very susceptible to three isolates M. anisopliae. In the end of the trials, fungal growth was detectable as early as 2 until 4 days of observation. Infection process with microphoto-graph and Scanning Electro Microscopy showed attachment, germination and penetration, extrusion and conidiogenesis fungal form.   Keywords: Infection, Tetranychus kanzawai, Metarhizium anisopliae, Light micros-copy, Scanning Electro Microscopy (SEM)
IDENTIFICATION OF POTENTIAL ENTOMOPATHOGENIC FUNGI OF Tetranychus kanzawai Kishida (Tetranychidae: Acarina) USING ITS-5.8s rDNA REGION AS MOLECULAR MARKER Sanjaya, Yayan; Ocampo, Virginia R.; Caoili, Barbara L.
AGRIVITA, Journal of Agricultural Science Vol 38, No 2 (2016): JUNE
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v38i2.560

Abstract

Fungi has been tested as one of the potential control agents for insect pests, which raises hopes for developing fungi as good biopesticides. The high variation within fungi species made taxonomic identification procedures more complex, thus molecular identification techniques are needed in addition to traditional morphological characteristics currently used as primary methods to classify fungi species. The objective of this research was to identify the species of the most pathogenic fungi to Tetranychus kanzawai Kishida using RAPD-PCR.  The internal transcribed spacer of 5.8s rDNA (ITS-5.8s rDNA) sequence of these fungal isolates were amplified using two sets of universal primers for ITS and then analyzed. Molecular identification showed that these  isolates had a higher  of similarity to Metarhizium anisopliae than Metarhizium flavoviride.
INFECTION PROCESS OF ENTOMOPATHOGENIC FUNGI Metarhizium anisopliae IN THE Tetranychus kanzawai (KISHIDA) (TETRANYCHIDAE: ACARINA) Sanjaya, Yayan; Ocampo, Virginia R.; Caoili, Barbara L.
AGRIVITA Journal of Agricultural Science Vol 35, No 1 (2013)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v35i1.180

Abstract

The importance of fungal attack to acarina has been observed but not yet on Tetranychus kanzawai. When infected mite, either within in a same  species or even in a different  species of  entomopathigenic probably  have  similar signs and simptoms to mites.  Characteristic of entomopathogenic fungus to Tetranychus kanza-wai was investigated. Three selected isolates of Metarhizium anisopliae, from Philippines and Indonesia were evaluated. The following aspects were investigated: (1) Bioassay tes of virulence, (2) Koch Postulate and (3) Infection process on each fungus against mite. Virulence of  Metarhizium anisopliae was 1.4 X 103, 7.2 X 102 , 5 X 102 PIB per ml on Ma4, Ma5 and Ma6, respectively. On Koch Postulates, Reinoculation and reisolation of all seven isolates from infected T. kanzawai showed definite signs of infection. This demonstrated its entomopathogenic capacity. In this experiment, adult mites were sprayed with 108 per ml concentrations of conidia observed under light microscope and Scanning Electron Microscopy (SEM). The result found  T. kanzawai was very susceptible to three isolates M. anisopliae. In the end of the trials, fungal growth was detectable as early as 2 until 4 days of observation. Infection process with microphoto-graph and Scanning Electro Microscopy showed attachment, germination and penetration, extrusion and conidiogenesis fungal form. Keywords: Infection, Tetranychus kanzawai, Metarhizium anisopliae, Light micros-copy, Scanning Electro Microscopy (SEM)
Co-Authors Virginia R. Ocampo YAYAN SANJAYA