Ethanol Extract of Stylissa carteri Induces Cell Death in Parental and Paclitaxel-Resistant Cervical Cancer Cells

Isna Nisrina Hardani (Undergraduate Medical Program, Faculty of Medicine, Universitas Padjadjaran)
Fachreza Aryo Damara (Undergraduate Medical Program, Faculty of Medicine, Universitas Padjadjaran)
Annisa Dewi Nugrahani (Undergraduate Medical Program, Faculty of Medicine, Universitas Padjadjaran)
Muhammad Hasan Bashari (Department of Biomedical Sciences, Division of Pharmacology and Therapy, Faculty of Medicine, Universitas Padjadjaran)

International Journal of Integrated Health Sciences
Vol 6, No 2 (2018)

Objective: To evaluate the cytotoxic effects of ethanol extract from marine sponge Stylissa carteri in both parental and paclitaxel-resistant HeLa cervical cancer cells. Methods: This was an experimental in-vitro study subjected ethanol extract from Stylissa carteri obtained from Pramuka Island, Kepulauan Seribu National Park, Jakarta. Both parental and paclitaxel-resistant HeLa cells were treated with ethanol extract followed by microscopic observation and MTT assay. The IC50 and drug curves were analyzed using four-parametric logistic regression by SigmaPlot for Windows version 12.0 from Systat Software Inc., USA.Results: Ethanol extract from Stylissa carteri triggered cell death-associated morphological changes in parental Hela cells. It demonstrated cytotoxic activities with an IC50 value of 1 ppm. Importantly, this extract also triggered cell death in paclitaxel-resistant HeLa cells. The IC50 of ethanol extract of Stylissa carteri was 4 ppm in paclitaxel-resistant HeLa cells.Conclusions: There is a potential cytotoxic activity of Stylissa carteri that is not only confirmed in parental HeLa cells but also in paclitaxel-resistant HeLa cells.Keywords: Cervical cancer, paclitaxel resistance, HeLa cells, cytotoxicity assay, Stylissa carteri DOI: 10.15850/ijihs.v6n2.1378