AKTIVITAS ANTIBAKTERI FRAKSI-FRAKSI DAUN Colocacia esculenta L. TERHADAP Salmonella thypi Dan Staphylococcus aureus SECARA BIOAUTOGRAPHY-TLC

Baits, Muzakkir
Herwin, Herwin
Ririn, Ririn

As-syifaa Jurnal Farmasi
Vol 8, No 2 (2016): AS-SYIFAA Jurnal Farmasi

Antibacterial activity test has been done on Colocacia esculenta L leaves fractions. The aim of the research was to determine the antibacterial activity of Colocacia esculenta L leaves fractions delivered from Pare-Pare District of South Sulawesi on Salmonella thypii and Staphyllococcus aureus by means of vacuum liquid chromatography and Bioautographic-TLC. Results of fractionations of the ethanol extract by the former method using eluent of 100 mL n-hexane, n-hexane:ethyl acetate (10:1; 1:1), 100 mL ethyl acetate, ethyl acetate:methanol (10:1; 1:1) and 100 mL methanol were obtained 7 fractions. Bioautographic-TLC assay then carried out on the obtained fractions they were fraction 3,4,5,6 and 7 respectively and obtained Rf value 0.80 was possessed antibacterial activity on both tested bacteria.