JURNAL KIMIA SAINS DAN APLIKASI
Vol 22, No 3 (2019): Volume 22 Issue 3 Year 2019

Isolasi Senyawa Flavonoid dari Fraksi Amil Alkohol Daun Mangga Golek (Mangifera indica L. cv. Golek)

Maulina Dwi Haryani (Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University Jl. Prof. Soedarto, SH., Tembalang, Semarang)
Enny Fachriyah (Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University Jl. Prof. Soedarto, SH., Tembalang, Semarang)
Dewi Kusrini (Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University Jl. Prof. Soedarto, SH., Tembalang, Semarang)



Article Info

Publish Date
31 May 2019

Abstract

Mango plant (Mangifera indica L.) is a tropical fruit plant with high nutrition in the Anacardiaceae family. One of the several varieties of mango plants spread throughout Indonesia, namely mango golek. This study aims to obtain flavonoid isolates and find out the types of flavonoids in the leaves of mango golek. In this study, maceration was carried out to obtain ethanol extract, then followed by phytochemical tests, removal of chlorophyll using distilled water (1: 1), hydrolysis using 2N HCl, followed by liquid-liquid extraction using ethyl acetate and amyl alcohol. In the amyl alcohol fraction, flavonoid was separated using gravity column chromatography with a mixture of eluent n-butanol:n-hexane:methanol (4:2:1). Phytochemical tests showed positive results for alkaloids, saponins, tannins, quinones and flavonoids. UV-Vis spectrophotometer analysis provides absorption at a wavelength of 263 nm while FTIR analysis showed the presence of O-H, C=O, C-O-C aliphatic, and C-O alcohol groups. LC-MS/MS instrument analysis showed m/z of 300 which is the molecular mass of isoflavone compound.

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Journal Info

Abbrev

ksa

Publisher

Subject

Chemical Engineering, Chemistry & Bioengineering Chemistry Engineering

Description

urnal Kimia Sains dan Aplikasi (p-ISSN: 1410-8917) and e-ISSN: 2597-9914) is published by Department of Chemistry, Diponegoro University. This journal is published four times per year and publishes research, review and short communication in field of ...