Staphylococcus aureus is one of the causes in the case of Foodborne Disease, with the resulting toxintriggering the occurrence of Foodborne Intoxication. The purpose of this study was to identify multidrugresistant patterns and detect Methicillin Resistance Staphylococcus aureus (MRSA) in cow’s milk in EastJava, Indonesia. A total of 170 cow’s milk samples were taken from dairy farms on 3 Districs, then thebacteria were cultured and purified using Mannitol Salt Agar media. Resistance tests were carried out with5 antibiotics on Mueller Hinton Agar media and Methicillin Resistant Staphylococcus aureus (MRSA) byOxacillin Resistance Screen Agar Base (ORSAB). The results showed there were 76 (44.7%) S. aureus, thenantibiotic testing showed 13 (17.1%) S. aureus isolates had multidrug resistant (MDR) properties on theantibiotics tested and 84.6% isolates with MDR confirmed MRSA properties by ORSAB. It was concludedthat the raw milk can be a potential reservoir for MDR and MRSA strains to threat public health.
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