Validasi dan Penetapan Kadar Rhodamin B pada Lipstik yang Beredar di Sekitar Universitas Jember dengan Metode KLT-Densitometri (Validation TLC-densitometry method for determination of Rhodamine B in Lipstick at Jember University Area)

Risa Wahyu Ananda (Fakultas Farmasi Universitas Jember Jln. Kalimantan 37, Jember 68121)
Nia Kristiningrum (Fakultas Farmasi Universitas Jember Jln. Kalimantan 37, Jember 68121)
Yuni Retnaningtyas (Fakultas Farmasi Universitas Jember Jln. Kalimantan 37, Jember 68121)

e-Journal Pustaka Kesehatan
Vol 2 No 1 (2014)

Rhodamine B is one of the synthetic dyes that forbidden in cosmetics. The study was conducted to validate TLC-densitometry method for determination Rhodamin B in lipstick at Jember University area. Samples was taken by purposive sampling method. The analyte were dissolved with ethanol 70% and cromatographed on silica Gel GF 254 TLC plate using etil asetat : metanol : amoniak in the ratio (15:3:3) (v/v/v). Then, validation and determination sample can be done using TLC-densitometry with maximum wavelength 554 nm. Method was found linear over the concentration range of 6 – 32 ng with correlation coefficient of 0,9965. Spesificity showed calculation of purity and identity more than 0.99. The limits of detection and quantitation limits obtained 0.558 and 1.86 ng/spot. The precision test which includes precision repeatability and intermediate precision, amounting to RSD 6.57% and test accuracy , resulting % recovery and RSD value of 97.74 % ± 2.54% . Based on these results lipstick sample purposively sampled none containing rhodamine B and safe consumer use. Keywords: TLC - densitometry , lipstick , rhodamine B , the validation method of analysis