PENGARUH PENAMBAHAN ION Fe3+ TERHADAP AKTIVITAS XILANASE DARI Trichoderma viride

Sulistyaningtyas, Ardyan Sukma
Prasetyawan, Sasangka
Sutrisno, Sutrisno

Jurnal Ilmu Kimia Universitas Brawijaya
Vol 2, No 2 (2013)

Xylanase is an enzyme that catalyzes the hydrolysis reaction substrate xylan (hemicellulose) into xylose. This study was conducted to determine the effect of Fe3+ on the activity of xylanase from Trichoderma viride using corn strove as an inducers. Effect of the addition of Fe3+ ions on xylanase activity was determined by comparing the xylanase activity with the addition of Fe3+ ions and xylanase activity without the addition of Fe3+ ions. Xylanase activity was determined by measuring the reducing sugar xylose spectrophotometrically using 3,5-dinitrosalisilat acid reagent (DNS). The results showed that the addition of Fe3+ ions in the concentration range of 5-35 mM can increase the activity of xylanase Keywords: Xylanase, Fe3+, Trichoderma viride, Corn Strove