Selection of bacteria inducing calcium carbonate precipitation for self-healing concrete application

Ridwan Syarif (Department of Mining Engineering, Faculty of Mining and Petroleum Engineering, Institut Teknologi Bandung)
Intan Nurul Rizki (Department of Environmental Engineering, Faculty of Science and Technology, Universitas Airlangga Geomicrobiology-Biomining and Biocorrosion Laboratory, Microbial Culture Collection Laboratory, Biosciences and Biotechnology Research Centre, Institut)
Ridho Kresna Wattimena (Department of Mining Engineering, Faculty of Mining and Petroleum Engineering, Institut Teknologi Bandung)
Siti Khodijah Chaerun (Geomicrobiology-Biomining and Biocorrosion Laboratory, Microbial Culture Collection Laboratory, Biosciences and Biotechnology Research Centre, Institut Teknologi Bandung Department of Metallurgical Engineering, Faculty of Mining and Petroleum Engine)

Current Research on Biosciences and Biotechnology
Vol. 1 No. 1 (2019)

A modification of bacterial medium using calcium lactate pentahydrate was developed for calcium carbonate precipitation. A total of five strains of bacteria were cultivated on the solution medium containing nutrient broth and calcium lactate pentahydrate. In this study, the variation of 3.2 mM and 16.2 mM of calcium lactate pentahydrate was used to obtain the optimum condition for bacterial growth. The results showed that isolated strains CPB 1, CPB 3, and CPB 5 with medium containing nutrient broth and 3.2 mM calcium lactate pentahydrate gave the optimum growth, pH and Eh, thus being favourable for the process of calcium carbonate precipitation. Hence, this will be useful for self-healing concrete.