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SITI KHODIJAH CHAERUN
Laboratory of Biogeosciences, Mining and Environmental Bioengineering, Research Division of Genetics and Molecular Biotechnology, School of Life Sciences and Technology, Institut Teknologi Bandung, Jalan Ganesha 10, Bandung 40132, Indonesia Center fo
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Earth & Planetary Sciences Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

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Mercury (Hg)-Resistant Bacteria in Hg-Polluted Gold Mine Sites of Bandung, West Java Province, Indonesia SITI KHODIJAH CHAERUN; SAKINAH HASNI; EDY SANWANI; MAELITA RAMDANI MOEIS
Microbiology Indonesia Vol. 6 No. 2 (2012): June 2012
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1736.523 KB) | DOI: 10.5454/mi.6.2.2

Abstract

In the present study, ten mercury-resistant heterotrophic bacterial strains were isolated from mercurycontaminated gold mine sites in Bandung, West Java Province, Indonesia. The bacteria (designated strains SKCSH1- SKCSH10) were capable of growing well at ~200 ppm of HgCl except for strain SKCSH8, which was able to grow at 550 ppm HgCl . The bacteria were mesophylic and grew optimally at 1% NaCl at neutral pH with the optimal growth temperature of 25-37 ºC. Phenotypic characterization and phylogenetic analysis based on the 16S rRNA gene sequence indicated that the isolates were closely related to the family Xanthomonadaceae, Aeromonadaceae, and Pseudomonadaceae and they were identified as Pseudomonas spp., Stenotrophomonas sp., and Aeromonas sp. Eight bacterial strains were shown to belong to the Pseudomonas branch, one strain to the Stenotrophomonas branch and one strain to the Aeromonas branch of the ã-Proteobacteria. Phylogeny based on their 16S rRNA gene sequences indicated that four of the isolates (SKCSH1, SKCSH4, SKCSH7, SKCSH9) could be classified as representatives of four novel species in the genus Pseudomonas that were allocated to P. moraviensis (96.96% similarity) and P. plecogossicida (94.53, 96.61, and 96.73% similarity). Four other isolates could be allocated to P. plecogossicida (97.57 and 98.66% similarity) and P. hibiscicola (99.97% similarity), one isolate to Stenotrophomonas africana (99.69% similarity), and one other isolate to Aeromonas hydrophila subsp. ranae (99.43% similarity). The findings of this study provide the first information of the phylogenetically-diverse Hg-resistant bacteria in the Hg-polluted sites of Indonesia that may be highly useful for developing in situ bioremediation or detoxification of Hg-contaminated sites in Indonesia.
Removal of sulfur and ash from Indonesian coal by indigenous mixotrophic bacteria Mubdiana Arifin; Edy Sanwani; Siti Khodijah Chaerun
Current Research on Bioscences and Biotechnology Vol. 1 No. 2 (2020)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/10.5614/crbb.2019.1.2/QNZG5857

Abstract

Coal is one of the alternative fuels which potentially provides most of the domestic energy needs. The steam-powered electric generator (PLTU) is a sector that dominates the utilization of domestic coal. Sulfur contained in the coal is an element impurity apart from other contaminants such as ash, soil, rocks, and minerals. The combustion of high-sulfur from coal produces SO 2 which can interfere the human health, such as causing tightness in the respiratory tract, as well as the environment by causing acid rain and corrosion on plant equipment. Various efforts have been made by reducing the levels of sulfur to minimize the negative impact caused by coal combustion. The utilization of bacteria for biodesulfurization has been developed and widely studied as an alternative treatment to remove the sulfur and ash from the coal. Therefore, the purpose of this study was to remove the sulfur and ash from coal using various types of indigenous bacteria by the biomining method. The current study used coal from East Kalimantan of Indonesia with a total sulfur and ash content of 2.56 and 7.21%, respectively. The indigenous bacteria used in this study consisted of five bacterial isolates identified as Citrobacter murliniae, Dietzia psychralcaliphila, Pseudomonas aeruginosa, Alcaligenes faecalis, Bacillus altitudinis. The results showed that the bacterium C. murliniae was able to eliminate the sulfur by 19.61%, which was higher than the other bacterial isolates and remove ash from coal by 1.75%. The bacteria D. psychralcaliphila, P. aeruginosa, A. faecalis, B. altitudinis were capable of eliminating 16.49, 8.30, 3.61, 8.89% of total sulfur and 4.03, 4.56, 5.29, 4.21 of ash content in coal, respectively.
Biocorrosion Behaviour of Carbon Steels by Tropical Microbes in the Presence of Corrosion-Inhibiting Bacterium Siti Khodijah Chaerun; Intan Nurul Rizki; Wahyu Ardi Hartomo; Bambang Widyanto
HAYATI Journal of Biosciences Vol. 30 No. 1 (2023): January 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.1.1-16

Abstract

A set of microbiologically induced corrosion was carried out on different types of carbon steels: AISI 1006 and API 5L X52, iron-oxidizing bacterium and sulfur-oxidizing bacterium, a mixed culture of Alicyclobacillus ferrooxydans SKC/SAA-2 and Aspergillus niger and Comamonas thiooxydans SKC/SAA-1, in the presence of the corrosion-inhibiting bacterium, Pseudomonas plecoglossicida. According to the immersion test experiments, weight loss of API 5L X52 was lower (0.06-0.27%) than AISI 1006 (0.14-0.32%). AISI 1006 showed more detrimental localized pitting corrosion than API 5L X52. During the longer incubation time, the corrosion-inhibiting bacterium was more homogenous and compact, which affected the specimen surface more protective characteristics. The 2-week-old biofilm effectively protected the API 5L X52, as indicated by the low amount and more negligible pitting corrosion. This study will be the first report on the biocorrosion behaviour of carbon steels using different corrosion-causing microbes in the presence of the corrosion-inhibiting bacterium.
Selection of bacteria inducing calcium carbonate precipitation for self-healing concrete application Ridwan Syarif; Intan Nurul Rizki; Ridho Kresna Wattimena; Siti Khodijah Chaerun
Current Research on Bioscences and Biotechnology Vol. 1 No. 1 (2019)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2019.1.1/ZDYD8792

Abstract

A modification of bacterial medium using calcium lactate pentahydrate was developed for calcium carbonate precipitation. A total of five strains of bacteria were cultivated on the solution medium containing nutrient broth and calcium lactate pentahydrate. In this study, the variation of 3.2 mM and 16.2 mM of calcium lactate pentahydrate was used to obtain the optimum condition for bacterial growth. The results showed that isolated strains CPB 1, CPB 3, and CPB 5 with medium containing nutrient broth and 3.2 mM calcium lactate pentahydrate gave the optimum growth, pH and Eh, thus being favourable for the process of calcium carbonate precipitation. Hence, this will be useful for self-healing concrete.
Biomineralization Biotechnology Utilizing Lysinibacillus sphaericus to Improve Mechanical Properties of Mortar Ridwan Syarif; Siti Khodijah Chaerun; Ridho Kresna Wattimena
HAYATI Journal of Biosciences Vol. 31 No. 1 (2024): January 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.1.48-58

Abstract

Biomineralization has notably enhanced the qualities of cement-based materials, particularly through bacterial-facilitated calcite precipitation via calcium lactate oxidation. However, existing research mainly targets self-healing aspects, with little focus on bio-based mortar properties. Consequently, this study provides a comprehensive examination of the enhancements in dry density, ultrasonic pulse velocity (UPV), and flexural strength, achieved through the application of a novel indigenous bacterial strain (Lysinibacillus sphaericus strain SKC/VA-1) from Indonesia, coupled with the incorporation of calcium lactate pentahydrate as an additive. A total of six mortar samples were prepared to investigate the influence of bacteria on the properties of mortar through biomineralization. The samples included plain mortar (M), mortar mixed with calcium lactate pentahydrate (ML), mortar mixed with a 10% v/v bacterial inoculum (MB1), mortar mixed with calcium lactate pentahydrate and a 10% v/v bacterial inoculum (MLB1), mortar mixed with a 20% v/v bacterial inoculum (MB2), and mortar mixed with calcium lactate pentahydrate and a 20% v/v bacterial inoculum (MLB2). The employment of a distinct bacterial strain for oxidizing calcium lactate represents an innovative aspect of the current study. The presence of organic calcium was found to have no adverse effects on the mortar matrix. Optimal inoculum concentrations of bacteria (10% v/v), in conjunction with calcium lactate pentahydrate, yielded superior mechanical properties. Mineralogical characterization via X-ray diffraction and microstructural analysis through scanning electron microscopy substantiated the incidence of calcite precipitation, which facilitated pore infilling and consequently augmented both the ultrasonic pulse velocity and the flexural strength of the mortar.
Unlocking copper flotation efficiency: the interaction of Citrobacter sp. strain SKC-4 with chalcopyrite concentrate as a potential eco-friendly reagent alternative Sanwani, Edy; Wahyuningsih, Tri; Winarko, Ronny; Chaerun, Siti Khodijah
Current Research on Biosciences and Biotechnology Vol. 6 No. 1 (2024)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2024.6.1/9BYQTYQ7

Abstract

This research explores the viability of bioflotation as an eco-friendly alternative to conventional chemical reagents in processing copper sulfide ores. It primarily focuses on the interactions between bacterial cells and chalcopyrite concentrate. A key objective is to identify bacterial strains suitable for use as bioflotation reagents. The study specifically examines the interaction between Citrobacter sp. strain SKC-4, a bacterium native to sulfur-rich environments, and chalcopyrite (CuFeS2) concentrates over a 30-day period. This bacterium is known for its ability to produce biosurfactants and oxidize iron and sulfur, making it an ideal candidate for various roles in bioflotation, such as biocollectors, biodepressants, and biofrothers. Experimental setups involved mixing chalcopyrite concentrates (-200+325 mesh, 25% w/v) with the bacterial strain (10% v/v) in modified LB media under aerobic conditions with constant shaking. Periodic sampling facilitated subsequent analyses. Results indicated biosurfactant production by the bacteria, as evidenced by the detection of hydroxyl (OH) groups, amine (NH) groups, and glycosidic bonds (C-O-C), along with the identification of hydrophilic C=O groups through FTIR (Fourier transform infrared) analysis, suggesting the presence of biodepressant, biocollector, and biofrother properties. Surface tension measurements consistently showed values below that of water (71 mN/m), supporting the biosurfactants' potential as flotation biofrothers and biocollectors. These findings suggest that employing Citrobacter sp. strain SKC-4 could substantially reduce the environmental impact of using chemical reagents in the flotation process. This study not only proposes a sustainable substitute for traditional flotation reagents but also demonstrates the potential of bioflotation to improve the efficiency and environmental sustainability of copper sulfide ore processing. The adoption of microorganisms as bioflotation reagents could transform the mineral processing industry by minimizing chemical use and environmental impact, contributing to more sustainable mining practices.
Co-Authors Arifin, Mubdiana Bambang Widyanto EDY SANWANI EDY SANWANI Edy Sanwani Intan Nurul Rizki MAELITA RAMDANI MOEIS Ridho Kresna Wattimena Ridho Kresna Wattimena Ridwan Syarif Ridwan Syarif SAKINAH HASNI Tri Wahyuningsih Wahyu Ardi Hartomo Winarko, Ronny