INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY
Vol 3 No 1 (2021): Laboratory innovation : The challenge for medical laboratory

Comparation Between Mac conkey and Coconut Water Medium as a Growth Medium for Escherichia coli

Endah Prayekti (Department of Medical Laboratory Technology, Faculty of Health, Universitas Nahdlatul Ulama Surabaya, Surabaya, Indonesia)
Suliati Suliati (Department of Medical Laboratory Technology, Poltekkes Surabaya, Surabaya, Indonesia)
Dwi Agustin Wulandari (Department of Medical Laboratory Technology, Faculty of Health, Universitas Nahdlatul Ulama Surabaya, Surabaya, Indonesia)



Article Info

Publish Date
28 Apr 2021

Abstract

Escherichia coli is the bacteria that can cause diarrhea in humans and often used as a parameter of stool environmental pollution. Culture of E. coli from the sample often requires Mac Conkey as commercial media which is able to distinguish it from other bacteria in the Enterobacteriaceae group. Commercial media such as Mac Conkey certainly has a price that is quite expensive because of its ability as a growth medium for Enterobacteriaceae. Therefore, in the study tested natural ingredients that can be used for growth media, such as coconut water. The purpose of this study was to compare the ability of Mac Conkey media and coconut water to support the growth of E. coli. This research is an experimental study with a completely randomized design. The concentration of coconut water tested was 0%, 20%, 40%, 60%, 80%, and 100%. The results showed that at the concentration of coconut water 20% to 60% the number of E. coli colonies on coconut water media was slightly below the Mac Conkey Agar media, while in coconut water a concentration of 80% showed a greater number of colonies than Mac Conkey. The Mann Whitney test showed a significant difference between the number of colonies on 80% coconut water media and Mac Conkey Agar, which was equal to 0.004 (p < 0.05). Based on these results, coconut water has the potential to be used as a growth medium for E. coli.

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Journal Info

Abbrev

IJMLST

Publisher

Subject

Health Professions Social Sciences

Description

Contributions will be considered for publication in Indonesian Journal of Medical Laboratory Science and Tehnology (IJMLST) concern kind from research, involvement and theory to functioning matters, education and training. The very wide spectrum of its topics includes: dosimeter, instrument ...