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<![CDATA[PENURUNAN JUMLAH BAKTERI KULIT MANUSIA DENGAN PERLAKUAN WUDHU DECREASING NUMBER OF HUMAN SKIN BACTERIA BY WUDHU TREATMENT ]]>
<![CDATA[Prayekti, Endah]]>
<![CDATA[ BIOMA Vol 1, No 2 (2016): BIOMA : JURNAL BIOLOGI DAN PEMBELAJARAN BIOLOGI ]]>
Publisher : <![CDATA[Prodi Pendidikan Biologi FKIP Unmuh Jember ]]>
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DOI: 10.32528/bioma.v1i2.441
<![CDATA[Wudhu sebagai aktifitas rutinitas yang dilakukan oleh muslim sebelum melakukan ibadah telah diteliti memiliki manfaat dalam menjaga kesehatan tubuh. Kulit sebagai bagian tubuh manusia yang terpapar langsung dengan perlakuan wudhu, merupakan barier pertama tubuh dari lingkungan luar dan dari masuknya mikroba yang berkolonisasi di permukaan kulit. Adanya proses membasuh kulit dengan air memungkinkan adanya terlepasnya bakteri dari kulit sebagai akibat kontak bakteri kulit dengan air. Penelitian ini bertujuan untuk mengetahui perbedaan jumlah bakteri kulit pada sebelum dan sesudah perlakuan wudhu. Penelitian ini memiliki dua tahapan, tahapan pertama membandingkan jumlah bakteri kulit pada kelompok muslim dan non muslim. Pada tahap kedua, dilakukan penghitungan jumlah bakteri kulit responden muslim pada lima titik waktu pengamatan. Penghitungan bakteri kulit dilakukan dengan mengambil sampel usap dari permukaan kulit pada ventral lengan reponden untuk kemudian dilakukan penghitungan jumlah bakteri dengan seri pengenceran yang sesuai. Perbandingan data kelompok muslim dan non muslim dianalisis secara deskriptif. Sedangkan data pengamatan jumlah bakteri sebelum dan sesudah wudhu pada lima titik waktu kemudian dianalisis statistik menggunakan t-test berpasangan. Hasil penelitian ini menunjukkan adanya pengaruh perlakuan sebelum dan sesudah wudhu terhadap jumlah bakteri kulit (p=0.006). Kata kunci: Wudhu, Bakteri Kulit, Hitungan Cawan.]]>
<![CDATA[PENYULUHAN PEMANFAATAN HERBAL MEDICINE DALAM RANGKA PENCEGAHAN PENYAKIT DIABETES MELLITUS BAGI KADER KESEHATAN DI MASYARAKAT DESA GELANG, KECAMATAN TULANGAN, SIDOARJO ]]>
<![CDATA[Wulandari, Devyana Dyah]]>;
<![CDATA[Prayekti, Endah]]>
<![CDATA[ JURNAL PENGEMBANGAN KOMUNITAS Vol 2 No 1 (2018): August ]]>
Publisher : <![CDATA[JURNAL PENGEMBANGAN KOMUNITAS ]]>
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<![CDATA[Masyarakat masih banyak yang belum sadar pentingnya menjaga kesehatan demi mencegah penyakit degeneratif dimasa tua. Apalagi pada jaman sekarang begitu banyak makanan siap saji yang berpotensi menimbulkan banyak penyakit, salah satunya adalah diabetes mellitus. Tim pengabdian masyarakat memberikan penyuluhan untuk meningkatkan pemhaman dan wawasan tentang pentingnya pemanfaatan herbal medicine dalam rangka pencegahanpenyakit diabetes mellitus. Tercapainya target dari kegiatan pengabdian kepada masyarakat ini, terbukti dari antusiasnya para peserta mengikuti penyuluhan dari mulai sampai berakhirnya acara serta banyaknya pertanyaan daripara peserta. Sedangkan tercapainya tujuan dan manfaat dari penyuluhan tentang pemanfaatan herbal medicine bagipasien pre diabetes memberikan wawasan bagi masyarakat Desa Gelang Kabupaten Sidoarjo tentang pentingnya perankonsumsi tanaman herbal dalam menunjang penurunan kadar glukosa darah adalah menjadi tolak ukur keberhasilanpenyuluhan ini.]]>
<![CDATA[CHITOSAN AS ANTIFUNGAL IN CHANNA STRIATA COLLAGENCHITOSAN FOR WOUND HEALING ]]>
<![CDATA[Andini, Ary]]>;
<![CDATA[Prayekti, Endah]]>
<![CDATA[ Jurnal Ilmu Kesehatan dan Kesehatan Vol 3 No 2 (2019): AUGUST ]]>
Publisher : <![CDATA[UNUSA Press ]]>
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DOI: 10.33086/mhsj.v3i2.1197
<![CDATA[The Snakehead fish contains high protein that was mostly used as a treatment during healing process in either invasive or passive surgery. Therefore, it could be developed as well as a mixture of wounds dressing for skin healing. The aim of this study was to know the effect of combination of concentrations between chitosan and collagen of Snakehead fish (Channa striata) skin and scales on fungal total number recovered from the composite. Snakehead fish skin and scales was treated by soaking in 2% HCl solvent for 48 hours to obtain collagen from its filtrate. Filtrate obtained continued to neutralize with 1 M NaOH until soluble collagen appeared. Collagen obtained in this study then mixed with 2% chitosan liquid to make wound dressing in various concentrations group. Combination of colagen mixture in this study were chitosan liquid only as control, 25% collagen-75% chitosan (C1), 50% collagen-50% chitosan (C2) and 75% collagen-25% chitosan (C3). The study results showed that on Control, C1 and C2 group there was no fungal contamination, but on C3 group there was contamination of fungal with total counting about 2,43 x 103 CFU. Based on statistically test showed that there was discrepancy for each group with p-value was 0,02 (p<0,05).]]>
<![CDATA[Pengolahan Limbah Organik Rumah Tangga Menggunakan Keranjang Takakura di Desa Simo Angin-Angin, Kecamatan Wonoayu, Kabupaten Sidoarjo ]]>
<![CDATA[Prayekti, Endah]]>;
<![CDATA[Riza, Mochammad Faishal]]>;
<![CDATA[Nandasari, Anggy Dwipa]]>;
<![CDATA[Salimi, Syafrina Maulidyatus]]>;
<![CDATA[Navis, Khiliah]]>;
<![CDATA[Pratama, Nizar Revandini]]>
<![CDATA[ PROSIDING SEMINAR NASIONAL PENGABDIAN KEPADA MASYARAKAT Vol. 3 No. 1 (2023): Prosiding Seminar Nasional Pengabdian Kepada Masyarakat : MEMAKSIMALKAN POTENSI ]]>
Publisher : <![CDATA[Universitas Nahdlatul Ulama Surabaya ]]>
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DOI: 10.33086/snpm.v3i1.1240
<![CDATA[Setiap harinya, sampah organik dihasilkan dari limbah rumah tangga dengan jumlah yang tidak sedikit. Sampah organik merupakan bahan buangan yang berasal dari makhluk hidup, baik manusia, hewan, maupun tumbuhan. Berdasarkan hal tersebut, perlu adanya suatu usaha untuk mengolah sampah organik rumah tangga salah satunya dengan membuat pupuk kompos. Salah satu metode pengolahan pupuk kompos yang cocok dilakukan dalam skala rumah tangga adalah dengan keranjang Takakura. Takakura adalah metode pengomposan yang memiliki kelebihan dan dapat diaplikasikan pada lahan yang sangat sempit, ruang dapur keluarga, atau kamar kost. Metode pelaksanaan yang digunakan dalam kegiatan ini adalah penyuluhan secara langsung. Pada penyuluhan tersebut juga dilakukan pelatihan (demonstrasi) cara pembuatan keranjang Takakura oleh anggota tim. Hasil yang diperoleh setalah dilakukan demosntrasi persentase pengethuan meningkat sebnyak 5%. Kegiatan ini bermanfaat untuk meningkatkan kemampuan kader desa dalam memberikan pelayanan Kesehatan bagi warga secara umum, dan lingkup keluarga kader sendiri secara khusus.]]>
<![CDATA[Comparation of n-Heksan and Etanol in Bawang Dayak (Elutherine palmifolia L. Merr) Extraction for Inhibiting Pseudomonas aeruginosa ]]>
<![CDATA[Putri, Ananda Ghufron Yuana]]>;
<![CDATA[Prayekti, Endah]]>
<![CDATA[ Medicra (Journal of Medical Laboratory Science/Technology) Vol 6 No 2 (2023): December ]]>
Publisher : <![CDATA[Universitas Muhammadiyah Sidoarjo ]]>
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DOI: 10.21070/medicra.v6i2.1662
<![CDATA[Pseudomonas aeruginosa is one of the bacteria that causes nosocomial infections. The incidence of nosocomial infections caused by Pseudomonas aeruginosa is around 10-15%. Nosocomial infections caused by these bacteria are increasingly difficult to treat. This happens because more and more strains are resistant to several antibiotics (Multidrug Resistance), so the selection of antibiotics against Pseudomonas aeruginosa is very difficult. This study aims to determine and analyze the effect of using polar and nonpolar solvents on Dayak onion bulb extract on the inhibitory ability of Pseudomonas aeruginosa. The method used in this study was Kirby-Bauer disk diffusion using 8 sample and 5 replication with assay solvent 96% ethanol and n-hexane extract of Dayak onion bulbs (Eleutherine palmifolia L. Merr) with concentration variations of 75%, 80%, and 85%. Positive control used ciprofloxacin 5 µg and positive control DMSO 10%. The results showed that on phytochemical screening of solvent ethanol 96% contained flavonoids and n-hexane contained triterpenoids. Ethanol 96% solvent obtained a higher presentase yield than n-heksan solvents and the highest inhibition zone diameter was 85% concentration in 96% ethanol solvent. The results of statistical analysis with Mann Whitney test showed a significant difference between the treatment groups (p<0.05).]]>
<![CDATA[Kesehatan Reproduksi Santri, Perempuan Sehat Remaja Kuat di Pondok Pesantren Wahid Hasyim Bangil ]]>
<![CDATA[Junaedy, M Dwinanda]]>;
<![CDATA[Nasir, M]]>;
<![CDATA[Umaha, Farida]]>;
<![CDATA[Laili, Uliyatul]]>;
<![CDATA[Prayekti, Endah]]>;
<![CDATA[Sadza, Lailatus]]>;
<![CDATA[Tauziah, Amalia]]>;
<![CDATA[Prisnidiawati, Ajeng]]>;
<![CDATA[Baihaqi, Muhammad Azhari]]>;
<![CDATA[Rizki Jalil, Ike Wasilatur]]>;
<![CDATA[Setyana Yuli, Azizah Ari]]>
<![CDATA[ Amalee: Indonesian Journal of Community Research and Engagement Vol 4 No 1 (2023): Amalee: Indonesian Journal of Community Research and Engagement ]]>
Publisher : <![CDATA[LP2M INSURI Ponorogo ]]>
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DOI: 10.37680/amalee.v4i1.2121
<![CDATA[Adolescence is a unique and vital developmental phase to build the foundation of health. During this period, maturation is about the reproductive system, so adolescents must understand reproductive health. According to WHO, to grow and develop with good health, adolescents need some information, including sex education, the opportunity to improve life skills, equality in health services, effective and supportive environment. This community service aims to provide health education to students at PP Wahid Hasyim Bangil. This community service was implemented at PP Wahid Hasyim Bangil on May 21 and 22 May 2022. The target of this community service is students at PP Wahid Hasyim, totaling 12 students. The method used is presentation and discussion in an online conference and a questionnaire to measure students' knowledge about reproductive health in pre and post-webinar. The results of data analysis using Wilcoxon showed that the p-value was 0.04, indicating a difference before and after the webinar on reproductive health. Community service effectively increases students' knowledge about reproductive health, so this event can do routine and make the adolescents community in Islamic boarding schools.]]>
<![CDATA[Optimalization of NaOH concentration in alkaline lysis method on quality and quantity of Candida albicans DNA ]]>
<![CDATA[Putri, Nasya Desinta]]>;
<![CDATA[Prayekti, Endah]]>
<![CDATA[ Journal of Indonesian Medical Laboratory and Science Vol 5 No 2 (2024): In Progress ]]>
Publisher : <![CDATA[Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia ]]>
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DOI: 10.53699/joimedlabs.v5i2.222
<![CDATA[Background: The concentration of NaOH in the alkaline lysis method needs to be optimized, especially when isolating DNA for its application in PCR which has not been maximized using the Candida albicans fungus which has a complex cell wall and is difficult to break. Thus, this optimization is expected to increase the efficiency of the alkaline lysis DNA isolation method in fungal species. Objective: The purpose of this studied was to determine the effect of optimizing the concentration of NaOH in the alkaline lysis method on the quality and quantity of Candida albicans DNA. Materials and Methods: This researched is experimental by performed DNA isolation used alkaline lysis method used NaOH concentrations of 1.5 N, 1.75 N, and 2.0 N and controlled (NaOH 0.2 N). Calculation of DNA quality and quantity using a nanodrop spectrophotometer. Results: The results showed the ordered of obtained the high to low DNA quality (A260/280) was NaOH concentration 2.0 N>1.75 N>1.5 N>0.2 N (1.86±0.44-0.95±0.18) while for DNA quantity was NaOH concentration 2.0 N>1.75 N>1.5 N>0.2 N (187.7±58.3-9.6±3.5 ng/?l). Conclusion: There is an effect of optimizing NaOH concentration on the lysis of fungal cell walls in the alkaline lysis method, namely increased the purity valued and concentration of sample DNA isolation results gradually. The use of 2.0 N NaOH concentration produced the best quality and quantity of DNA, namely 1.86±0.44 for DNA quality and 187.7±58.3 for DNA quantity.]]>
<![CDATA[Modification of CTAB incubation time in the isolation of DNA from Staphylococcus aureus ]]>
<![CDATA[Awaliyah, Arisma Putri]]>;
<![CDATA[Prayekti, Endah]]>
<![CDATA[ Journal of Indonesian Medical Laboratory and Science Vol 5 No 2 (2024): In Progress ]]>
Publisher : <![CDATA[Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia ]]>
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DOI: 10.53699/joimedlabs.v5i2.224
<![CDATA[Background: DNA was an essential component in organisms that regulates biological activity. DNA analysis began with isolating pure, highly concentrated DNA, often used the CTAB method to isolate DNA from bacteria. DNA quality affected by incubation timed and temperature as well as its purity. The right incubation timed could optimize the degradation of proteins in the cell wall and maximize the released of DNA from the cell. DNA purity is good if the 260/280 absorbance ratio valued is 1.8-2.0. Objectives: This studied aimed to obtained the highest concentration and quality of DNA by modifying the incubation time of CTAB used Staphylococcus aureus DNA. Materials and Methods: This studied uses quantitative methods with the typed in experimental researched. The isolation stage included cell lysis with CTAB buffer and proteinase-K, purification stage with chloroform: isoamyl alcohol (24:1), DNA precipitation stage with isopropanol. Results: The average result of DNA purity obtained from the incubation treatment for 10 minutes was 1.89, for 20 minutes incubation was 1.46, and for 30 minutes incubation was 1.23. The average DNA concentration obtained from the incubation treatment for 10 minutes was 153 ng/µL, for 20 minutes incubation was 78.2 ng/µL, and for 30 minutes incubation was 26 ng/µL. Electrophoresis results at 10 minutes incubation time clearly visible DNA bands, 20 minutes incubation time DNA bands looked faint, and 30 minutes incubation time is not visible DNA bands. Conclusions: Based on the results of the studied, modification of CTAB incubation time with an incubation time of 10 minutes is the result of the highest concentration and quality of DNA using Staphylococcus aureus DNA.]]>
<![CDATA[CHITOSAN AS ANTIFUNGAL IN CHANNA STRIATA COLLAGENCHITOSAN FOR WOUND HEALING ]]>
<![CDATA[Andini, Ary]]>;
<![CDATA[Prayekti, Endah]]>
<![CDATA[ Jurnal Ilmu Kesehatan dan Kesehatan Vol 3 No 2 (2019): AUGUST ]]>
Publisher : <![CDATA[UNUSA Press ]]>
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DOI: 10.33086/mhsj.v3i2.1197
<![CDATA[The Snakehead fish contains high protein that was mostly used as a treatment during healing process in either invasive or passive surgery. Therefore, it could be developed as well as a mixture of wounds dressing for skin healing. The aim of this study was to know the effect of combination of concentrations between chitosan and collagen of Snakehead fish (Channa striata) skin and scales on fungal total number recovered from the composite. Snakehead fish skin and scales was treated by soaking in 2% HCl solvent for 48 hours to obtain collagen from its filtrate. Filtrate obtained continued to neutralize with 1 M NaOH until soluble collagen appeared. Collagen obtained in this study then mixed with 2% chitosan liquid to make wound dressing in various concentrations group. Combination of colagen mixture in this study were chitosan liquid only as control, 25% collagen-75% chitosan (C1), 50% collagen-50% chitosan (C2) and 75% collagen-25% chitosan (C3). The study results showed that on Control, C1 and C2 group there was no fungal contamination, but on C3 group there was contamination of fungal with total counting about 2,43 x 103 CFU. Based on statistically test showed that there was discrepancy for each group with p-value was 0,02 (p<0,05).]]>
<![CDATA[Herbal Plant Education and Hydroponic Training of Vertical and Wick System in Limited Space ]]>
<![CDATA[Andini, Ary]]>;
<![CDATA[Prayekti, Endah]]>;
<![CDATA[Romdloni , Muhammad Afwan]]>;
<![CDATA[Rhomadhoni , Muslikha Nourma]]>;
<![CDATA[Sofaria Munir, Ratna]]>
<![CDATA[ Community Development Journal Vol 8 No 1 (2024): Community Development Journal ]]>
Publisher : <![CDATA[Universitas Nahdlatul Ulama Surabaya ]]>
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DOI: 10.33086/cdj.v8i1.5619
<![CDATA[The program "Empowering the Community of Simo Angin-Angin Village, Sidoarjo through the Use of Natural Materials to Achieve Health Independence" was implemented at the Simo Angin-Angin Village Hall, Krian District, Sidoarjo Regency in May-June 2023. This Community Empowerment Program (Community Service) carries a preventive and consists of a series of activities, including counseling about the use of natural materials based on the recommendations of the Koran, considering that the majority of Simo Angin-Angin village residents are Muslim. This activity also includes training in farming using the hydroponic method. The aim of this program is to strengthen health independence in Simo Angin-Angin Village in order to improve collective welfare. The Community Service program in Simo Angin-Angin Village was attended by 22 participants, consisting of health cadres and the general public. The implementation is divided into 2 stages, namely counseling regarding the Black Cumin herbal plant and training regarding vertical hydroponic procedures, as well as the use of plastic bottles with the Wick system. The evaluation results showed that participants' understanding was very good, with 95% of them achieving a score of 100, while 5% obtained a score of 80. The evaluation results of participant satisfaction with the implementation of the extension showed very good scores for the topic material and delivery of the material by resource persons on herbal plant education and hydroponic training. This Community Service activity provides skills for the community to grow crops on narrow land using a hydroponic system]]>
