JURNAL ATOMIK
Vol 5 No 1 (2020)

SCREENING LIPASE FROM ENDOPHYTIC BACTERIA FROM STEAM OF (Costus speciosus (J.Koenig) Sm) AND OPTIMUM WORKING CONDITION

Asjayani Kurnia Sari (Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Mulawarman)
Winni Astuti (Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Mulawarman)
Djihan Ryn Pratiwi (Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Mulawarman)

Article Info

Publish Date
28 Sep 2020

Abstract

Endophytic bacteria are bacteria that live in plant tissues such as the roots, leaves, and stems of plants. This research was conducted to screen lipase-producing endophytic bacteria from Pacing stem (Costus speciosus (J.Koenig) Sm.) and determine the optimum working conditions. Screening of lipase-producing bacteria on nutrient agar media containing olive oil and Rhodamine B showed that one isolate was able to produce lipase. The crude lipase extract produced from endophytic bacteria worked optimally at a pH of 7, the temperature of 40℃, and a substrate concentration of 2%. Keywords : Costus speciosus (J.Koenig) Sm., Endophytic bacteria, Lipase, Optimum Activity

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Journal Info
JURNAL ATOMIK
Website

Abbrev

JA

Publisher

Universitas Mulawarman

Subject

Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Environmental Science

Description

Jurnal Atomik (JA) is published twice a year (March and August) by Department of Chemistry, Faculty of Mathematics and Natural Sciences, Mulawarman University. Jurnal Atomik is a media for graduate students of Department of Chemistry to publish their research articles in Chemistry field including ...