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All Journal JURNAL ATOMIK
Djihan Ryn Pratiwi
Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Mulawarman
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Environmental Science

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SCREENING LIPASE FROM ENDOPHYTIC BACTERIA FROM STEAM OF (Costus speciosus (J.Koenig) Sm) AND OPTIMUM WORKING CONDITION Asjayani Kurnia Sari; Winni Astuti; Djihan Ryn Pratiwi
JURNAL ATOMIK Vol 5 No 1 (2020)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

Endophytic bacteria are bacteria that live in plant tissues such as the roots, leaves, and stems of plants. This research was conducted to screen lipase-producing endophytic bacteria from Pacing stem (Costus speciosus (J.Koenig) Sm.) and determine the optimum working conditions. Screening of lipase-producing bacteria on nutrient agar media containing olive oil and Rhodamine B showed that one isolate was able to produce lipase. The crude lipase extract produced from endophytic bacteria worked optimally at a pH of 7, the temperature of 40℃, and a substrate concentration of 2%. Keywords : Costus speciosus (J.Koenig) Sm., Endophytic bacteria, Lipase, Optimum Activity
Co-Authors Asjayani Kurnia Sari Winni Astuti