HAYATI Journal of Biosciences
Vol. 28 No. 2 (2021): April 2021

Identification of Potential Bacteria on Several Lakes in East Java, Indonesia Based on 16S rRNA Sequence Analysis

Achmad Rodiansyah (Department of Biology, Faculty of Mathematics and Natural Science, Universitas Negeri Malang, Malang)
Ainul Fitria Mahmudah (Department of Biology, Faculty of Mathematics and Natural Science, Universitas Negeri Malang, Malang)
Mastika Marisahani Ulfah (Department of Biology, Faculty of Mathematics and Natural Science, Universitas Negeri Malang, Malang)
Uun Rohmawati (Department of Biology, Faculty of Mathematics and Natural Science, Universitas Negeri Malang, Malang)
Dwi Listyorini (Department of Biology, Faculty of Mathematics and Natural Science, Universitas Negeri Malang, Malang)
Eko Agus Suyono (Faculty of Biology, Universitas Gadjah Mada, Yogyakarta)
Sitoresmi Prabaningtyas (Department of Biology, Faculty of Mathematics and Natural Science, Universitas Negeri Malang, Malang)



Article Info

Publish Date
01 Apr 2021

Abstract

Four bacterial isolates from Ranu Pani and Ranu Grati in east java had been revealed to be potentials to produce IAA (PIS isolate), phosphate solubilizer (GPS isolate), cellulose hydrolysis (PSS isolate) and, amylum hydrolysis (PAS), two dominant bacterial isolates from Rani Pani (PØD isolate) and Ranu Grati (GØD isolate) which were co-cultured with microalgae promoted microalgae growth, yet its taxonomical position has not been clearly known. The aim of this study was to identify those bacterial isolates using 16S rRNA barcode. This research conducted by gDNA isolation, the 16S rRNA sequence was amplified using 27F and 1492R primers. Reconstructed phylogenetic trees and genetic distance analysis showed that the isolate PIS and PSS identified as Bacillus cereus Group closely related to Bacillus paramycoides. PAS isolate identified as Bacillus subtilis Group closely related to Bacillus amyloliquefaciens, GPS isolate identified as novel species in genus Enterobacter, and two dominant isolates (PØD and GØD) identified as Enterobacter cloacae complex closely related to Enterobacter cloacae. The genomic approach and additional phenotypes-examination are required to clarify its taxonomical position.

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Journal Info

Abbrev

hayati

Publisher

Subject

Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

Description

HAYATI Journal of Biosciences (HAYATI J Biosci) publishes articles and short communication in tropical bioscience fields such as development, biotechnology, biodiversity and environmental issues. HAYATI J Biosci covers wide range of all life forms topics including virus, microbes, fungi, plants, ...