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All Journal HAYATI Journal of Biosciences Journal of Tropical Biodiversity and Biotechnology Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Canrea Journal: Food Technology, Nutritions, and Culinary Journal Berkala Penelitian Hayati Makara Journal of Science Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan
Eko Agus Suyono
Faculty Of Biology, Universitas Gadjah Mada, Jalan Teknika Selatan, Kampus UGM, Yogyakarta 55281, Indonesia; Center Of Excellence For Microalgae Biorefinery, Universitas Gadjah Mada, Sekip K1A, Yogyakarta 55281, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

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Identification of Potential Bacteria on Several Lakes in East Java, Indonesia Based on 16S rRNA Sequence Analysis Achmad Rodiansyah; Ainul Fitria Mahmudah; Mastika Marisahani Ulfah; Uun Rohmawati; Dwi Listyorini; Eko Agus Suyono; Sitoresmi Prabaningtyas
HAYATI Journal of Biosciences Vol. 28 No. 2 (2021): April 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.2.136

Abstract

Four bacterial isolates from Ranu Pani and Ranu Grati in east java had been revealed to be potentials to produce IAA (PIS isolate), phosphate solubilizer (GPS isolate), cellulose hydrolysis (PSS isolate) and, amylum hydrolysis (PAS), two dominant bacterial isolates from Rani Pani (PØD isolate) and Ranu Grati (GØD isolate) which were co-cultured with microalgae promoted microalgae growth, yet its taxonomical position has not been clearly known. The aim of this study was to identify those bacterial isolates using 16S rRNA barcode. This research conducted by gDNA isolation, the 16S rRNA sequence was amplified using 27F and 1492R primers. Reconstructed phylogenetic trees and genetic distance analysis showed that the isolate PIS and PSS identified as Bacillus cereus Group closely related to Bacillus paramycoides. PAS isolate identified as Bacillus subtilis Group closely related to Bacillus amyloliquefaciens, GPS isolate identified as novel species in genus Enterobacter, and two dominant isolates (PØD and GØD) identified as Enterobacter cloacae complex closely related to Enterobacter cloacae. The genomic approach and additional phenotypes-examination are required to clarify its taxonomical position.
Biochemical compounds and sub-chronic toxicity test of Chlorella sp. and Spirulina sp. isolated from Glagah Coastal Water Slamet Widiyanto; Mulyati Sarto; Laksmindra Fitria; Rahadian Yudo; Eko Agus Suyono
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 24 No 1 (2018): December 2018
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1127.852 KB) | DOI: 10.23869/37

Abstract

Microalgae are microscopic photosynthetic organisms found in marine and freshwater environments. This organism is a eukaryotic group of polyphyletic and very diverse. This study investigated the biochemical composition and sub-chronic test of Chlorella Sp. and Spirulina Sp. isolated from Glagah coastal water (Yogyakarta, Indonesia) as a preclinical study to create potential nutritional supplement for human health. Microalgae culture was analyzed for biochemical composition using several methods: AAS for metal analysis (Pb, Cu, Fe, Mg, Zn, and Hg), GC-MS and UV spectrophotometer for fatty acid profiles analysis (lenolenic acid, eicosatrienoic acid, arachidonic acid, linoleic acid, oleic acid, eicosenoic acid, EPA, DHA, omega 6 and 9), Alkaloids, Steroids, Tanin and Saponin. The next step is to do a sub-chronic test of microalgae biomass using experimental animals. The result shows that Spirulina sp. and Chlorella sp. contain 16 and 10 different biochemical compounds respectively. Furthermore, organic nutritional properties in both of microalgae are relatively high with a rich biochemical profile of Fe and nutritious poly-unsaturated fats (such as linolenic acid, arachidonic acid , omega 6, and 9). Regarding sub-chronic toxicity test, it was clear that Spirulina and Chlorella did not affect the blood profile. These findings suggest that both of Glagah strain microalgae are considered to have great potential as a multi-nutritional human health supplement.
Effect of chlorophyll in alginate-based edible film in inhibiting spoilage of fish snacks Eko Nurcahya Dewi; Asmi Citra Malina A.R. Tassakka; Mochammad Yuwono; Eko Agus Suyono; Lukita Purnamayati; Jamaluddin Fitrah Alam
jurnal1 VOLUME 5 ISSUE 1, JUNE 2022
Publisher : Hasanuddin University Food Science and Technology Study Program

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/canrea.v5i1.571

Abstract

Edible films are environmentally biodegradable materials used for food packaging. The edible green alga Caulerpa racemosa has antimicrobial properties; however, its chlorophyll-based bioactive compounds can be damaged when heated so it is prepared in microcapsules. Our research evaluated the effect of Caulerpa microcapsules on an alginate-based edible film on film properties and food spoilage. The microcapsules were used at concentrations of 0%, 0.5%, 1%, and 1.5%. The edible film was measured for film properties, total phenolic content (TPC), antioxidant activity (DPPH), functional group (FTIR) and microstructure (SEM). Food spoilage was evaluated on a popular fish-based product (fish bubble snacks). Bacterial strains of Escherichia coli and Staphylococcus aureus were used to evaluate antimicrobial effectiveness of the edible films. The addition of Caulerpa microcapsules had no significant negative effect on physical properties of the alginate-based film, while the smoother and more homogenous surface should enhance the barrier properties of the film. The slow and evenly distributed release of active compounds from the microcapsules increased resistance to Rhizopus sp. and significantly reduced the proliferation of E. coli but not S. aureus on coated fish snacks. Caulerpa racemosa can be used to enhance the effectiveness of alginate-based films in delaying spoilage and could extend product shelf-life
The Effect of Liquid Organic Fertilizer “Bio Ferti” Application on the Growth Rate of Spirulina platensis by Using Haldane Model M Iqbal Maulana Ginting; Eko Agus Suyono; Mochammad Donny Koerniawan; Lucia Tri Suwanti; Ulfah Juniarti Siregar; Arief Budiman
Journal of Tropical Biodiversity and Biotechnology Vol 7, No 2 (2022): August
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.68944

Abstract

This experimental research was performed to observe the influence of an agricultural liquid organic fertilizer called Bio Ferti on the growth and biomass of Spirulina platensis, aiming at replacing inorganic fertilizer with the liquid organic one. The cultivation of the microalgae was conducted over seven days at Nogotirto Algae Park. The liquid organic fertilizer, namely Bio Ferti, was obtained from the Faculty of Biology, Universitas Gadjah Mada, and prepared to have doses of 2, 4, 6, 8, and 10 mL. For comparison, an inorganic fertilizer with the same doses was also prepared. The variables to be observed were cell density, dry cell weight, and growth kinetics. The culture medium conditions observed were temperature, pH, and salinity (the optimum salinity was 20 ppt). The growth kinetic analysis was performed mathematically using numerical simulations using the Contois and the Haldane models. This research’s results showed that Bio Ferti affected the growth rate of Spirulina platensis. With a dose of 2 mL, it became the optimum medium which produced the highest density and dry weight of 1.78x106 cells/mL and 160 mg/mL, respectively. Meanwhile, the inorganic fertilizer with a dose of 10 mL produced the highest density and dry weight of 2,13x105 and 80 mg/mL, respectively. The temperature ranged from 28 to 31°C, while the pH ranged from 8.01 to 9.02 for each medium. The suitable model to describe the growth kinetics of Spirulina platensis was the Haldane model.
The Effect of Acidic pH on Growth Kinetics, Biomass Productivity, and Prima-ry Metabolite Contents of Euglena sp. Nurafifah, Istini; Hardianto, Muhammad Andhi; Erfianti, Tia; Amelia, Ria; Maghfiroh, Khusnul Qonita; Kurnianto, Dedy; Siswanti, Dwi Umi; Sadewo, Brilian Ryan; Putri, Renata Adaranyssa Egistha; Suyono, Eko Agus
Makara Journal of Science Vol. 27, No. 2
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Euglena is a microalga with the potential to be an environmentally friendly renewable energy resource. The pH value is a crucial factor in micro-algal cultivation. Changes in pH affect the growth and development of microalgae, including the production of biomass and primary metabolites, such as proteins, carbohydrates, and lipids. In this study, Euglena sp. was grown on Cramer-Myers medium and subjected to various acidic conditions. This study aimed to determine the effect of pH on the growth kinetics, biomass, carbohydrate, lipid, and protein contents of Euglena sp. The Euglena sp. culture was optimized at various pH values of 2.5, 3.5, and 4.5. The results were analyzed by one-way analysis of variance at a 95% confidence level, followed by Duncan’s multiple range test. As results, Euglena sp. had the best growth rate, the greatest biomass, and the highest carbohydrate, protein, and lipid contents at pH 3.5 compared to the other pH conditions. The average biomass in the pH 3.5 treatment was 1.600 ± 0.229 g/L, and the carbohydrate, protein, and lipid contents were 5.983 ± 0.056 g/L, 0.196 ± 0.023 µg/mL, and 0.300 ± 0.020 g/L, respectively.
Cobalamin and Thiamine Effect on Microalgae Biomass Production in the Glagah Consortium Setyaningrum, Tri Wahyu; Budiman, Arief; Suyono, Eko Agus
Journal of Tropical Biodiversity and Biotechnology Vol 8, No 3 (2023): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.81949

Abstract

The Glagah consortium is a mixed culture of various microalgae and bacteria isolated from Glagah Beach, Yogyakarta. Cobalamin and thiamine, which are given by symbiotic bacteria, are assumed will increase biomass. This study aimed to determine the effect of cobalamin and thiamine on microalgae biomass production in the Glagah consortium. The microalgae of Glagah consortium were cultivated for 10 days with vancomycin and gentamicin antibiotic as treatment and without antibiotics as a control. The parameters measured included the number of bacterial colonies, cobalamin and thiamine levels measured by LC-MS, chlorophyll a and b levels, cell density of microalgae and dry biomass. The highest level of cobalamin and thiamine was in the Glagah consortium without antibiotics. Cobalamin and thiamine increased in the exponential phase along with the increasing Staphylococcus sp. colonies. The Quantity of Staphylococcus sp. colonies in the exponential phase was 62.105 (cfu/mL). The level of cobalamin in the exponential phase was 2.33 µg/L and the level of thiamine in the exponential phase was 49.71 µg/L. The highest productivity dried weight biomass was 0.0134 g/L/day in the day-6th on the Glagah consortium without antibiotics. This result showed that microalgae and bacterial interaction was mutualism symbiosis involving cobalamin and thiamine that increased in the exponential phase along with the increasing Staphylococcus sp. colonies. This interaction was able to increase biomass microalgae.
Growth Kinetic Modelling of Efficient Anabaena sp. Bioflocculation Rahmawati, Amalia; Rohmawati, Irma; Nurafifah, Istini; Sadewo, Brilian Ryan; Suyono, Eko Agus
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 1 (2024): March
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.82196

Abstract

Bioflocculation is a harvesting technique that employs flocculant agents such as bacteria and microalgae. The benefit is the absence of a chemical-added flocculant. Because bacteria need a particular medium, microalgae flocculant agents are more effective. This study used Anabaena sp. to collect fat, protein, and carbohydrates from the Glagah consortium. Three replications of those microalgae were grown in 300 ml of Bold Basal Medium culture for eight days. On the day of harvest, flocculant microalgae (Anabaena sp.) and non-flocculant microalgae (Glagah) were combined to accomplish flocculation. On the day of harvest, parameters were observed by combining Anabaena sp. with the Glagah consortium in the ratios 1: 1, 0.5: 1, and 0.25: 1. There were three times of each parameter test. Utilizing a wavelength of 750 nm, the proportion of precipitation was calculated spectrophotometrically. Bligh and Dyer were used to measure the lipids. The phenol sulfate technique was used to calculate the amount of carbohydrates. By employing the Bradford method, proteins were quantified. Biofocculation percentages and carbohydrate content were optimum on a ratio of 0.25:1. Lipid and protein content were optimum on a ratio of 1:1.
Bioremediation of Mercury- Polluted Water in Free Water Surface-Constructed Wetland System by Euglena sp. and Echinodorus palifolius (Nees & Mart.) J.F. Macbr. Siswanti, Dwi Umi; Daryono, Budi Setiadi; Petrus, Himawan Tri Bayu Murti; Suyono, Eko Agus
Journal of Tropical Biodiversity and Biotechnology Vol 8, No 3 (2023): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.88143

Abstract

Mercury accumulation in the aquatic environment can be highly harmful. The body takes mercury vapor through the lungs, then absorbs mercury metal through the digestive system, and then the blood carries the metal to the brain. Bioremediation is the process of breaking down or converting harmful compounds into non-toxic forms, which can be accomplished through phytoremediation or phycoremediation. The goal of this study was to examine the growth and anatomy of Euglena sp. after being cultured in the mercury-containing FWS-CW waste treatment system. The ability of Euglena sp. and Echinodorus palifolius to bioremediate mercury at different concentration as well as association and non-association treatments. This study was carried out in a bioreactor known as FSW-CW (Free Water Surface-Constructed Wetlands). Plant growth (plant height and number of leaves), chlorophyll content, diameter of root and petiole, metaxylem diameter of root, petiole, and leaves, cortical thickness of root and leaves, and petiole anatomy were all measured. Water temperature, pH, salinity, and light intensity were all measured as environmental parameters. Mercury treatment reduced Euglena density (183.5 cells. mL-1103 in control and 12.6 cells. mL-1103 in 100 ppm mercury treatment) and number of E. palifolius leaves, but not plant height and chlorophyll. Root and petiole diameters were affected by the mercury treatment, petiole diameter decreased unless the concentration was 100 ppm, whereas root diameter actually increased. The diameter of the root metaxylem increased, but the petioles and leaves, as well as the thickness of the root cortex, did not provide a significant response. The growth of E. palifolius was still optimal in the presence of Euglena in mercury-containing medium. 
Bioflocculant Technology Implementation Using Navicula sp for Harvesting of Arthrospira Platensis Culture Suyono, Eko Agus; Satriayudistira, Jody Ashrib; Baihaqi, Yahya; Amelia, Ria
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 19, No 2 (2024): August 2024
Publisher : :Agency for Marine and Fisheries Research and Human Resources, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.883

Abstract

Bioflocculation, a microalgae harvesting method utilizing organisms likebacteria and microalgae as flocculant agents, necessitates careful selectionand ratio determination of bioflocculant agents. This study seeks to ascertainthe optimal balance of bioflocculant, Navicula sp., for metabolite productionand harvesting efficiency in Arthrospira platensis culture. Spanning three oneweek cultivation stages, the research commences with A. platensis cultureunder varying salinities (0, 15, 25, and 35 ppt) in the first stage, followed bypH variations (9, 10, 11, and 12) in the second stage. The optimal salinity-pHcombination identified in the first two stages is applied in the third stage,involving bioflocculant addition to culture and medium solutions at varyingratios (0.25:1, 0.5:1, 0.75:1, and 1:1 v/v). Precipitation efficiency andpigmentation were measured using spectrophotometry, while lipid contentwas determined by the Bligh Dyer extraction method. Carbohydrate contentwas assessed using the Phenol-Sulphuric Acid assay, and protein contentwas quantified using the Bradford protein assay. The findings indicate salinity,pH, and bioflocculant influence A. platensis growth, metabolite production,and harvesting efficiency. Optimal outcomes are achieved at 15 ppt salinity,pH 9, and a 1:1 ratio of bioflocculant, yielding 1.18x10-3 g of dry biomass,3.56x105 cells/mL of cell density, 2.9x100 g/L of carbohydrates, 3.63x100 g/Lof lipids, 9.97x10-3 mg/L of chlorophyll a+b, 8.71x10-1 mg/L of carotenoids,and 3.04x10-1 g/L of protein. These results suggest that salinity addition, highpH, and the addition of Navicula sp. as bioflocculant increase the harvestingprocess efficiency of A. platensis culture.
Effects of Copper on Euglena sp. Local Strains and Remediation Capabilities Ardipeni, Witia; Suyono, Eko Agus; Dewayanto, Nugroho; Mufida, Na’imatul; Erfianti, Tia; Amelia, Ria; Kurnianto, Dedy
HAYATI Journal of Biosciences Vol. 32 No. 2 (2025): March 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.2.387-394

Abstract

One of the well-known industries in Yogyakarta is the Kotagede silver craft, which produces heavy metal waste, one of which is copper. The copper content in the liquid waste of electroplating Kotagede silver crafts is 4.628 mg/L. At the same time, Indonesian Government Regulation No. 22 (2021) stipulates a critical limit of Copper (Cu) content in river and lake water, which is 0.2 mg/L. The purpose of the study was to analyze the effects of copper on local strains of Euglena sp. (growth, photosynthetic pigment production, and superoxide dismutase enzyme) as well as the effectiveness of Euglena sp. in copper remediation. The study was conducted by adding a copper solution obtained from CuSO4•5H2O to the culture medium of Euglena sp. The parameters observed in the study were growth (number of cells and biomass), photosynthetic pigment content (chlorophyll a, chlorophyll b, carotenoid total), Superoxide dismutase enzyme of Euglena sp. as well as the effectiveness of Euglena sp. in copper remediation. The results showed that copper inhibited the growth of Euglena sp. The highest growth was the control treatment (without adding copper to the culture medium). Likewise, the content of photosynthetic pigments and the culture of Euglena sp. exposed to copper were lower when compared to the control culture. The activity of the SOD enzyme is increased in cultures exposed to copper. The result of the research is Cu metal absorption efficiency by Euglena sp. by 21.93%, 10.93%, and 9.47% for a Cu concentration of 10 ppm, 15 ppm, and 20 ppm.
Co-Authors Achmad Rodiansyah Ainul Fitria Mahmudah Amalia Rahmawati Amelia, Ria Ardipeni, Witia Arief Budiman Arief Budiman Asmi Citra Malina Astiti, Adam Baihaqi, Yahya BUDI SETIADI DARYONO Dwi Listyorini Dwi Umi Siswanti Eko Nurcahya Dewi Erfianti, Tia Hardianto, Muhammad Andhi Himawan Tri Bayu Murti Petrus Irma Rohmawati Jamaluddin Fitrah Alam Karilanata, Khalid Erlangga Kurnianto, Dedy Laksmindra Fitria Larasati, Ersi Lucia Tri Suwanti, Lucia Tri Lukita Purnamayati Luthfiana, Dwi Hardianti M Iqbal Maulana Ginting Maghfiroh, Khusnul Qonita Mastika Marisahani Ulfah Mochammad Donny Koerniawan Mochammad Yuwono Mufida, Na’imatul Mulyati Sarto Nugroho Dewayanto, Nugroho Nurafifah, Istini Putri, Renata Adaranyssa Egistha Rahadian Yudo Rohmawati, Uun Sadewo, Brilian Ryan Satriayudistira, Jody Ashrib Sitoresmi Prabaningtyas Slamet Widiyanto Solly Aryza Tri Wahyu Setyaningrum, Tri Wahyu Ulfah Juniarti Siregar