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All Journal HAYATI Journal of Biosciences El-Hayah : Jurnal Biologi Indonesian Journal of Biotechnology Jurnal Biologi Tropis Jurnal Pengabdian Masyarakat Bhinneka Pena Emas
Rohmawati, Uun
Laboratory Of Microbiology, Department Of Biology, Faculty Of Mathematics And Natural Sciences, Universitas Negeri Malang, Semarang No.5 Malang 65145, Indonesia
Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Other

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Comparison of Secondary Metabolite Content of Pteris vittata L. in Baluran National Park and Malang and Its Effect on Environment Sulasmi, Eko Sri; Rohmawati, Uun; Amin, Achmad Makin
El-Hayah : Jurnal Biologi Vol 7, No 1 (2018): EL-HAYAH (VOL 7, NO 1 September 2018)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v7i1.7240

Abstract

Fern is cosmopolitan plants which are almost scattered in all parts of the world, one of which is found in Baluran National Park and in Malang. The potential and benefits of these ferns are quite important for agriculture and medicine because of the chemical compounds they have, especially in Pteris vitatta L. This study aimed to analyze secondary metabolites contained in P. vittata L. in Baluran National Park, Situbondo and in Malang. The samples used were leaves and rhizome P. vittata extracted using methanol 96%, followed by a qualitative test of the content of alkaloids, flavonoids, terpenoids, polyphenols, tannins, saponins, using phytochemical screening methods with several reagents. The results showed that the leaves and Rhizome P. vittata L.. in Baluran National Park, Situbondo were positively containing secondary metabolites of terpenoids, polyphenols, tannins, saponins, and alkaloids (Dragendorf and Bouchardat reagents), whereas flavonoids were not present in all samples. However, the results of P. vitatta L. phytochemical screening around Malang State University positively contained flavonoids, polyphenols, terpenoids, alkaloids (Wagner and Dragendorf reagents). The difference in results from these two places is because the secondary metabolite content in plants is affected by stressful environmental conditions such as soil texture where it is grown or is affected by the precursors of the secondary metabolites of the metabolites.
Identification of Potential Bacteria on Several Lakes in East Java, Indonesia Based on 16S rRNA Sequence Analysis Achmad Rodiansyah; Ainul Fitria Mahmudah; Mastika Marisahani Ulfah; Uun Rohmawati; Dwi Listyorini; Eko Agus Suyono; Sitoresmi Prabaningtyas
HAYATI Journal of Biosciences Vol. 28 No. 2 (2021): April 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.2.136

Abstract

Four bacterial isolates from Ranu Pani and Ranu Grati in east java had been revealed to be potentials to produce IAA (PIS isolate), phosphate solubilizer (GPS isolate), cellulose hydrolysis (PSS isolate) and, amylum hydrolysis (PAS), two dominant bacterial isolates from Rani Pani (PØD isolate) and Ranu Grati (GØD isolate) which were co-cultured with microalgae promoted microalgae growth, yet its taxonomical position has not been clearly known. The aim of this study was to identify those bacterial isolates using 16S rRNA barcode. This research conducted by gDNA isolation, the 16S rRNA sequence was amplified using 27F and 1492R primers. Reconstructed phylogenetic trees and genetic distance analysis showed that the isolate PIS and PSS identified as Bacillus cereus Group closely related to Bacillus paramycoides. PAS isolate identified as Bacillus subtilis Group closely related to Bacillus amyloliquefaciens, GPS isolate identified as novel species in genus Enterobacter, and two dominant isolates (PØD and GØD) identified as Enterobacter cloacae complex closely related to Enterobacter cloacae. The genomic approach and additional phenotypes-examination are required to clarify its taxonomical position.
Isolation and characterization of α ‐amylase encoding gene in Bacillus amyloliquefaciens PAS Achmad Rodiansyah; Sitoresmi Prabaningtyas; Mastika Marisahani Ulfah; Ainul Fitria Mahmuda; Uun Rohmawati
Indonesian Journal of Biotechnology Vol 26, No 4 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.61425

Abstract

Amylolytic bacteria are a source of amylase, which is an essential enzyme to support microalgae growth in the bioreactor for microalgae culture. In a previous study, the highest bacterial isolate to hydrolyze amylum (namely PAS) was successfully isolated from Ranu Pani, Indonesia, and it was identified as Bacillus amyloliquefaciens. That bacterial isolate (B. amyloliquefaciens PAS) also has been proven to accelerate Chlorella vulgaris growth in the mini bioreactor. This study aims to detect, isolate, and characterize the PAS’s α‐amylase encoding gene. This study was conducted with DNA extraction, amplification of α‐amylase gene with polymerase chain reaction (PCR) method with the specific primers, DNA sequencing, phylogenetic tree construction, and protein modeling. The result showed that α‐amylase was successfully detected in PAS bacterial isolate. The α‐amylase DNA fragment was obtained 1,468 bp and that translated sequence has an identity of about 98.3% compared to the B. amylolyquefaciens α‐amylase 3BH4 in the Protein Data Bank (PDB). The predicted 3D protein model of the PAS’s α‐amylase encoding gene has amino acid variations that predicted affect the protein’s structure in the small region. This research will be useful for further research to produce recombinant α‐amylase.
Potential of Bioactive Compounds of Allium sativum L. var. solo garlic Extract in Inhibiting InhA Protein in Mycobacterium tuberculosis Rohmawati, Uun; Amalia, Atikah
Jurnal Biologi Tropis Vol. 25 No. 2 (2025): April-Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i2.9119

Abstract

Tuberculosis (TB) is caused by the bacteria Mycobacterium tuberculosis. In general, TB is treated with compounds that inhibit the work of one of the enzymes in the bacteria Mycobacterium tuberculosis, namely the InhA enzyme. One of the herbal plants that has the potential to inhibit the InhA protein in the bacteria Mycobacterium tuberculosis is. Allium sativum L. var. solo garlic. This research aims to determine the bioactive compounds in Allium sativum L. var. solo garlic in inhibiting the InhA protein in Mycobacterium tuberculosis which can be used as an alternative drug in the treatment of TB through an in silico approach. The method used in this study was qualitative phytochemical screening and GCMS test on Allium sativum L. var. solo garlic extract, the bioactive compounds found were tested in silico through a molecular docking approach. The research results obtained that Allium sativum L. var. solo garlic contains alkaloids, flavonoids, tannins and steroids. Based on the results of the GCMS test, the bioactive compounds that have an area of ​​more than 5% are Heptadecene-(8)-Carbonic Acid-(1), 3-Deoxy-D-Mannonic Acid, 5-Hydroxymethylfurfural, Melezitose and Oleic acid. The results of molecular docking showed that the compound 3-Deoxy-D-mannonic acid had a binding affinity of -4.9, 5-Hydroxymethylfurfural -4.8, Oleic Acid -6.4, Nicotinamide-Adenine-Dinucleotide (Control) had a binding affinity of -11.4. Low binding affinity indicates that the compound can bind to the protein with little energy. The Gyps energy theory states that the smaller the energy produced from a bond between the ligand and its receptor, the more stable the bond is. The lowest binding energy to the InhA protein is in the control compound Nicotinamide-Adenine-Dinucleotide, Oleic Acid, 3-Deoxy-D-mannonic acid, 5-Hydroxymethylfurfural.
Pemberdayaan Masyarakat melalui Pelatihan Kreasi Olahan Mocaf sebagai Inovasi Pangan Lokal Kekinian di Desa Gununggangsir, Beji, Pasuruan Fauziah, Shaddiqah Munawaroh; Sutama, Daning Kinanti; Rohmawati, Uun
Jurnal Pengabdian Masyarakat Bhinneka Vol. 3 No. 4 (2025): Bulan Juli
Publisher : Bhinneka Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58266/jpmb.v3i4.304

Abstract

Tepung mocaf (Modified Cassava Flour) merupakan salah satu produk turunan singkong yang memiliki nilai gizi dan ekonomi tinggi, serta dapat menjadi alternatif bahan pangan lokal yang inovatif. Namun, pemanfaatan mocaf di masyarakat, khususnya di Desa Gununggangsir, Kecamatan Beji, Kabupaten Pasuruan, masih terbatas. Kegiatan pengabdian masyarakat ini bertujuan untuk memberdayakan masyarakat melalui pelatihan kreasi olahan mocaf dengan pendekatan kekinian, guna menarik minat generasi muda dan menciptakan peluang usaha rumahan. Pelatihan difokuskan pada pembuatan berbagai produk olahan seperti brownies mocaf, cookies mocaf, dan donat mocaf, dengan sentuhan tampilan dan rasa yang sesuai dengan tren kuliner masa kini. Metode pelatihan mencakup penyuluhan gizi dan potensi mocaf, demonstrasi resep, serta praktik langsung oleh peserta. Hasil kegiatan menunjukkan bahwa peserta tidak hanya mampu membuat olahan dengan baik, tetapi juga menunjukkan minat untuk mengembangkan usaha kecil dari produk tersebut. Kegiatan ini diharapkan dapat menjadi sarana untuk mengangkat kembali potensi pangan lokal melalui pendekatan inovatif dan meningkatkan kemandirian ekonomi masyarakat desa.
Gel Biodesel dari Pemanfaatan Limbah Kulit Pisang Kepok (Musa Paradisiacal) dan Minyak Jelantah sebagai Bahan Bakar Alternatif Mustofa, Bukhori; Rohmawati, Uun; Hawa Maharani, Aqina; Sal Sabila, Aura; Ali Mashabi, Muhammad
PenaEmas Vol 1 No 1 (2023): PenaEmas
Publisher : MAN 1 Pasuruan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.64688/jpe.v1i1.8

Abstract

Di Indonesia, produksi dan konsumsi minyak bumi semakin meningkat yang menyebabkan ketersediaannya semakin menipis. Oleh karena itu diperlukan alternatif sumber energi terbarukan. Salah satunya memanfaatkan limbah kulit pisang kepok (Musa paradisiacal) dan minyak jelantah menjadi biodesel. Penelitian ini bertujuan mengetahui proses dan kualitas biodesel yang dihasilkan limbah kulit pisang kepok dan minyak jelantah. Proses pembuatannya menggunakan metode esterifikasi-transesterifikasi. Rancangan penelitian terdapat lima perlakuan yaitu S0: solar murni, S1: biodesel kulit pisang, S2: biodesel minyak jelantah, S3 : biodesel kulit pisang kepok dan minyak jelantah (1:1), S4 : biodesel kulit pisang kepok dan minyak jelantah (2:1). Parameter diukur dari kualitas warna, lama pembakaran dan warna api. Hasil penelitian ini memiliki karakteristik, yaitu viskositasnya 2.31 mm2/s, flash point sebesar 130 0C dan densitas pada suhu 40 0C yaitu 850 kg/m3. Biodesel kulit pisang kepok memiliki karakteristik viskositas 2.80 mm2/s, flash point sebesar 130 0C dan densitas suhu pada 40 0C yaitu 870 kg/m3. Karakteristik gel biodesel yang dihasilkan setiap perlakuan S0, S1, S2, S3 dan S4 secara berurutan yaitu memiliki warna kuning jernih, kuning kecoklatan (++), kuning kecoklatan sedikit keruh (+++), kuning kecoklatan keruh (++++). Lama pembakaran per 10 mL pada perlakuan S0, S1, S2, S3 dan S4 secara berurutan yaitu pada suhu normal ±25 0C tidak bisa menyala, S1 lama pembakaran 2 menit 13 detik, S2 : 2 menit 3 detik, S3 : 2 menit 23 detik, sedangkan S4 : 2 menit 46 detik. Kesimpulan bahwa kulit pisang dan minyak jelantah bisa menghasilkan gel biodesel sehingga dapat menghasilkan sumber energy terbarukan. Kata kunci: biodesel, kulit pisang kepok, minyak jelantah. Abstract In Indonesia, the production and consumption of petroleum is increasing from year to year, causing its availability to decrease. Therefore an alternative is needed by developing renewable energy. One of them is by utilizing waste kepok banana peels (Musa paradisiacal) and used cooking oil to become biodiesel. This study aims to determine the process and quality of biodiesel produced from kepok banana skin waste and used cooking oil. The process of making kepok banana skin biodiesel and used cooking oil uses the esterification-transesterification methods. The research design consisted of 5 treatments, namely S0: pure diesel fuel, S1: banana peel biodiesel, S2: used cooking oil biodiesel, S3: kepok banana peel biodiesel and used cooking oil (1:1), S4: kepok banana peel biodiesel and used cooking oil (2 :1). Parameters are measured from the quality of the color, burning time and the color of the fire. The results of the research on used cooking oil biodiesel have characteristics, namely the viscosity is 2.31 mm2/s, the flash point is 130 0C and the density at 40 0C is 850 kg/m3. Kepok banana skin biodiesel has a viscosity characteristic of 2.80 mm2/s, flash point of 130 0C and density at 40 0C of 870 kg/m3. The characteristics of the biodiesel gel produced by each treatment S0, S1, S2, S3 and S4 sequentially are clear yellow, brownish yellow (++), slightly cloudy brownish yellow (+++), cloudy brownish yellow (++++) . Burning time per 10 mL in treatments S0, S1, S2, S3 and S4 sequentially i.e. at normal temperature ±25 0C can not ignite, S1 burning time 2 minutes 13 seconds, S2 : 2 minutes 3 seconds, S3 : 2 minutes 23 seconds , while S4 : 2 minutes 46 seconds. It can be concluded that banana peels and used cooking oil can produce biodiesel gel. Keywords: biodiesel, kepok banana peel, used cooking oil.
Co-Authors Achmad Rodiansyah Ainul Fitria Mahmuda Ainul Fitria Mahmudah Ali Mashabi, Muhammad Amalia, Atikah Amin, Achmad Makin Dwi Listyorini Eko Agus Suyono Eko Sri Sulasmi Hawa Maharani, Aqina Mastika Marisahani Ulfah Mastika Marisahani Ulfah Mustofa, Bukhori Sal Sabila, Aura Shaddiqah Munawaroh Fauziah Sitoresmi Prabaningtyas Sutama, Daning Kinanti