Perancangan primer gen lktB pada Fusobacterium necrophorum untuk analisis PCR

Rian Ka Praja (Biomedical Sciences Program, Faculty of Medicine, Udayana University Denpasar, Bali, Indonesia, 80113)
Reny Rosalina (Chemistry Department, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Jawa Barat, Indonesia, 40132)

Jurnal Sains dan Teknologi Peternakan
Vol 2 No 2 (2021): Jurnal Sains dan Teknologi Peternakan

Fusobacterium necrophorum is a pathogen causing disease in animals, especially cattle, goats, and sheep. F. necrophorum infection can result in a variety of necrotic conditions (necrobacillosis). This study aimed to design a pair of primers for detecting the leukotoxin B (lktB) gene expressed by F. necrophorum as diagnostic support. The lktB gene sequence was obtained from GenBank NCBI with accession number AF312861.3:685-2337. Furthermore, the sequence was used as a template for in silico primer design using Primer-BLAST. Primer candidates successfully designed were then analyzed for their secondary structure using NetPrimer. The results showed that forward primer set 6 (5'-TCGGATGCTGGAATGCTACTT-3') and reverse primer set 6 (5'-GGGCTCCCAAATCCTTACGA-3') were a favorable primer set with a product size of 228 bp. However, laboratory experiments need to be carried out to determine the optimal conditions for this primer set.