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All Journal Jurnal Pendidikan dan Pemberdayaan Masyarakat Aceh Journal of Animal Science Jurnal Sains Teh dan Kina Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Oceana Biomedicina Journal JPPM (Jurnal Pengabdian dan Pemberdayaan Masyarakat) Biomolecular and Health Science Journal Jurnal Sains dan Teknologi Peternakan Indonesian Journal of Applied Research (IJAR) OISAA Journal of Indonesia Emas

Reny Rosalina

Institut Ilmu Kesehatan Bhakti Wiyata Kediri

Author-ID : 3271057

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Education Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Mechanical Engineering Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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The oxidation reaction of quinine by potassium permanganate (KMnO4) Rosalina, Reny; Alni, Anita; Mujahidin, Didin; Santoso, Joko
Jurnal Penelitian Teh dan Kina Vol 18, No 2 (2015)
Publisher : Research Institute for Tea and Cinchona

Quinine was known widely as malarial drug and a bitter taste ini soft drink. Recently, quinine have been also applied in organo-catalysis process, separation and purification of chiral compounds from enantiomeric mixture by diasteromeric salt crystallization and chiral chromatography. Quinine contains various functional groups which easily transforms by oxidations. This research was to study the oxi-dation reaction of quinine by using potassium permanganate (KMnO4) as oxidator. The KMnO4 oxidation carried out under acidic con-ditions. The separation and purification of pro-ducts were done by radial chromatography with chloroform : metanol : tri ethyl amine (9:1:0.1) as eluent. The structure of products were cha-racterized by spectroscopic data including: in-frared spectroscopy, NMR, and mass spectro-metry as quinine-1-N-oxide and quininal.

Molecular Mechanism of Cholerae Toxin (ctx) in Causing Diarrhea Praja, Rian Kusuma Arinta; Rosalina, Reny
Oceana Biomedicina Journal Vol 1, No 2 (2018): Oceana Biomedicina Journal
Publisher : Universitas Hang Tuah

Vibrio cholerae is one of the pathogenic bacteria transmitted through contaminated food, especially seafood and contaminated beverages. V. cholerae produces cholerae toxin (ctx) which is encoded by the ctx gene located within its chromosome. This toxin has been recognized as one of the toxins responsible for cholera outbreaks. The mechanism of ctx gene expression is induced by environmental signals such as pH, osmolarity, temperature, bile, amino acids, and CO2. These signals will be a positive transcriptional factor to the ToxR gene that regulates the biogenesis of cholerae toxin. After cholerae toxin has been successfully expressed, V. cholerae uses a type II secretion (T2S) pathway to deliver cholerae toxin to the extracellular environment. Cholerae toxin consists of A and B subunits. The B subunit plays a role in attaching to the receptor Manosialosyl Ganglioside (GM1 ganglioside) and the A subunit plays a role in catalyzing ADP-ribosylation of Gs (stimulatory) proteins and turning them into active condition. The Gs proteins will convert the inactive adenilate cyclase (AC) into active AC. The increase of AC activity will increase the cyclic adenosine 3'5'-monophosphate (cAMP) concentration along the cell membrane. The cAMP then causes the active secretion of sodium (Na+), chloride (Cl-), potassium (K+), bicarbonate (HCO3-), and water (H2O) out of the cell into the intestinal lumen, resulting in large fluid losses and electrolyte imbalances. Keywords: Vibrio cholerae, cholerae toxin (ctx), ToxR gene, type II secretion (T2S), GM1 ganglioside, adenilate cyclase.

Preparation and Antifungal Activities of Chitosan from Apple-Snail (Pila Ampullacea) Shells as Preserving Agent for Postharvest Podang Mango (Mangifera Indica L.) Cv Podang Riska Surya Ningrum; Aisyah Ramadani; Reny Rosalina; Desy Yustiyani; Rio Altha Reyzando
Indonesian Journal of Applied Research (IJAR) Vol. 2 No. 2 (2021): Indonesian Journal of Applied Research (IJAR)
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/ijar.v2i2.120

Podang mango, an endemic mango from Kediri, has a short post-harvest shelf life that accelerates by physiological factors and pathogenic fungal contamination. Chitosan reported as an active compound for coating. The apple-snail was reported to contain chitosan in its shell. The purposes of this study are to synthesis chitosan from Pila ampullacea, examine anti-fungal activities to Fusarium oxysporum and Aspergillus ochraceus, and verify the effect of chitosan coating on shelf life and quality of Podang mango. The effect of chitosan coating on fruit quality was analyzed using three treatments which were negative control, chitosan, and chitosan-gelatin with 3 replications. The parameters measured were color, texture, aroma, water content, vitamin C, and weight loss. Results showed that chitosan synthesized from P. ampullacea had 53.56 degrees of deacetylation. Antifungal activity test of chitosan 2% had the highest inhibitory activity against F.oxysporum and A.ochraceus growth with 14.46 mm and 10.33 mm inhibition zone. Coating with chitosan statistically affected fruit quality for 14 days of storage (sig <0.05). Chitosan-gelatin coating is the best treatment in reducing weight loss (8.97%), maintain aroma (sweet fragrance), texture (soft), water content (84%), and vitamin C (0.182%), while the color (reddish yellow) was more influenced by Chitosan coating. Chitosan coated with gelatin is recommended for application on Podang mangoes.

THE EXISTENCE OF VIBRIO CHOLERAE IN INDONESIA: FROM ENVIRONMENTAL TO CLINICAL ASPECTS (A CONCISE REVIEW) Rian Ka Praja; Anggita Ratri Pusporini; Reny Rosalina; I Wayan Muda Suta Arta; I Dewa Made Sukrama; Ni Nengah Dwi Fatmawati
Jurnal PPI Dunia Vol 4 No 1 (2021)
Publisher : OISAA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52162/jie.2021.004.01.1

Vibrio cholerae is an infectious agent causing cholera disease with a high prevalence in various developing countries. V. cholerae is a pathogen with broad spectrum host that can infect humans and animals, especially aquaculture. The existence of this disease in Indonesia has long been identified in several outbreaks. Various reports in Indonesia have succeeded in finding the existence of V. cholerae in the environment, aquaculture, food and beverage, as well as in clinical cases of V. cholerae infection. The presence of V. cholerae in environment such as water source is commonly related with contamination. However, V. cholerae can be found in aquatic environment as this environment is natural habitat for V. cholerae. Thus, aquaculture is prone to be infected with V. cholerae because the presence of this pathogen is abundant in aquatic environment. Contaminated food and beverage are associated with hygiene and sanitation and human is commonly infected after consuming contaminated food or beverage. This brief review has the main focus to discuss the existence of V. cholerae from environmental to clinical aspects found in Indonesia.

In silico SYBR® green-based qPCR primer design for virulent protein C gene of Edwardsiella tarda Rian Ka Praja; Reny Rosalina
Aceh Journal of Animal Science Vol 6, No 3 (2021): November 2021
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/ajas.6.3.19989

Edwardsiella tarda infection in fish results in economic losses in aquaculture industries worldwide. Besides, this pathogen is also a zoonotic agent causing gastrointestinal disease in humans. Detection of virulence factors expressed by E. tarda is a key for molecular diagnosis. This study aimed to design SYBR®Green-based quantitative PCR (qPCR) primer for E. tarda virulent protein C (EvpC) gene. A sequence of EvpC with accession number AY424360.4:6500-6991 obtained from GenBank NCBI was selected as the basis for qPCR primer design. qPCR primers were designed using Primer3 online software. Further analysis related to the secondary structure of each primer was carried out using Beacon Designer Free Edition. Five pairs of qPCR primers were successfully designed with Primer3. Based on the results of Primer3 and Beacon Designer analysis, F primer pair 1 (5'-GAATCCACCGACGATAAGCACAAA-3') and R primer pair 1 (5'-GACACGCAGCACCGACATCA-3') were the most favorable primer set since this primer met the criteria in terms of length, melting temperature, GC content, and self complementarity. In addition, this primer set had the highest (closest to zero) -ΔG value of cross dimer, self dimer, and hairpin. It can be concluded that SYBR® Green-based qPCR primer set for EvpC has been successfully designed. However, laboratory optimization experiments are essential for this designed primer set.

Pelatihan pembuatan minuman berserat alami dari buah nanas dan nata de coco Aisyah Hadi Ramadani; Reny Rosalina; Riska Surya Ningrum
JPPM (Jurnal Pendidikan dan Pemberdayaan Masyarakat) Vol 7, No 2 (2020): November 2020
Publisher : Departement of Nonformal Education, Graduate Scholl of Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jppm.v7i2.32162

Salah satu sentra produksi nanas di Jawa Timur adalah di Dusun Puhrejo, Kecamatan Ngancar, Kabupaten Kediri. Nanas yang dihasilkan dijual dalam bentuk buah segar dan olahan, contohnya adalah sari nanas. Kualitas produk olahan sari nanas masih kurang maksimal sehingga pada kegiatan ini diberikan pengetahuan dan pelatihan tentang cara pengolahan buah nanas dalam bentuk minuman berserat. Minuman berserat dibuat dari campuran buah nanas yang kaya nutrisi dan nata de coco yang mengandung serat alami. Penambahan nata de coco juga berfungsi sebagai penstabil (emulsifier) agar produk minuman yang dihasilkan memiliki mutu yang lebih baik dan daya simpan yang lebih lama. Terdapat 2 tahapan kegiatan yang diikuti oleh 25 warga Dusun Puhrejo, yaitu penyuluhan dan pelatihan. Hasil yang diperoleh dari kegiatan ini adalah adanya peningkatan pengetahuan peserta mengenai pemanfaatan dan cara pengolahan nanas yang tepat sehingga tidak mengurangi kandungan gizi di dalamnya. Minat peserta untuk mengembangkan produk olahan nanas juga meningkat karena produk yang dihasilkan dapat memberi nilai tambah buah nanas. Produk olahan yang diperoleh dari kegiatan ini juga dijadikan produk unggulan UMKM Dusun Puhrejo. Training on produce a healthy beverage from pineapple fruits and nata de coco AbstractOne of the pineapple production centers in East Java is in Puhrejo village, Ngancar, Kediri. The pineapple was sold in the fresh and processing fruits, like pineapple juice. But the pineapple juice has low quality so this activity gave information and training about how to process pineapple to be a healthy beverage product. A healthy beverage produced from pineapple that contains many nutritions and nata de coco that contain natural fibre. The aim of addition nata de coco is as emulsifier therefore the beverage obtained from this project has better quality and longer shelf life. The activity was attended by 25 Puhrejo village residents and used two steps, that are counselling and training. The result of the activity is an increase in the community’s knowledge about the potential of pineapple and the right processing of it so the nutrition did not decrease. The desire of the community to process pineapple into a variety of products also increased because that product can provide added value to pineapple. The healthy beverage product from this activity will be a superior product from UMKM of Puhrejo village

Immunomodulator Effect of Lemongrass Extract (Cymbopogon nardus L.) to Increase Immune Cells as a Precaution Against SARS-CoV-2 Putri Ayu Ika Setiyowati; Rofiatun Solekha; Sri Bintang Sahara Mahaputra Kusuma Negara; Reny Rosalina
Biomolecular and Health Science Journal Vol. 4 No. 2 (2021): Biomolecular and Health Science Journal
Publisher : Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/bhsj.v4i2.26619

Introduction: In humans, Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV-2) can damage some tissue when the immune systems was decrease. Natural product from the plant often used to improve immune response against microorganism including virus. This study aimed to determine the potential antioxidant of lemongrass extract (C . nardus) with various dosage that can provide immunomodulatory effects and find an optimal dosage to be used. Methods: The method used observasional analytic, using animal model of 30 male mice strain BALB/C, weight 25-30 gram, divided into 5 groups; the positive control group was given 0.05 mL of 0.05% CMC within 14 days, negative control group was given IMBOOST® tablet 200 mg/kg body weight (bw) within 14 days, treatment groups were given C. nardus extract with various doses 50 mg/kg bw, 150 mg/kg bw, and 300 mg/kg bw. In day 21 all group were injected with 0,2 ml of pathogen bacterial (S. aureus). Blood samples were taken three times: 7th day, 14th day, and 21th day. Results: The results showed that lemongrass extract (C. nardus) was able to influence the leukocyte and lymphocytes count with significant different (p<0.05). The optimal dose is 150 mg/kg body weight. Conclusion: The antioxidant compounds that contain in the C. nardus extract have an ability to increasing the immune system in the dose 150 mg/kg bw , but in the dose 300 mg/kg bw became toxic that can make a skin injury or death in animal test.

Protective Effect of Lemongrass Extract (Cymbopogon nardus) on Spermatozoa Cells Induced by Lead Acetate Putri Ayu Ika Setiyowati; Rofiatun Solekha; Dwi Dianita Irawan; Reny Rosalina; Karin Alifia Rachmadani; Angella Ananda Saputra
Biomolecular and Health Science Journal Vol. 5 No. 1 (2022): Biomolecular and Health Science Journal
Publisher : Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/bhsj.v5i1.31450

Introduction: Male infertility can be caused by many factors. One of which is environmental pollution such as lead acetate. Lead acetate exposure can increase Reactive Oxygen Species (ROS), affecting spermatozoa quality. Natural antioxidants and flavonoid on Lemongrass (C. nardus) have a great potential for protecting the male reproductive system. This study aimed to analyze the effect of giving various dosages of C. nardus extract on the sperm quality of mice. Methods: The Balb/C male mice were divided into five equal groups. The negative control group was injected with 0.01 ml Na-CMC 0.5% within 40 days, and the positive control group was injected with 0.01 ml lead acetate 7 mg/kg BW within 5 days, the treatment groups were injected by 0.01 ml lead acetate 7 mg/kg body weight within 5 days and continuously injected by 0.01 ml of C. nardus extract with various dosage 25 mg/kg BW, 50 mg/kg BW, and 100 mg/kg BW within 35 days. At the end of the experiment, mice were sacrificed, and sperm suspensions were collected from cauda epididymal to measure the morphology, concentration, and motility.Results: The results showed that giving C. nardus extract could repair morphology, concentration, and motility of spermatozoa with significantly different (p<0.05). The 25 mg/kg BW dose has a good protective effect. Conclusion: The C. nardus extract can repair the decreasing sperm quality caused by lead acetate exposure.

Perancangan primer gen lktB pada Fusobacterium necrophorum untuk analisis PCR Rian Ka Praja; Reny Rosalina
Jurnal Sains dan Teknologi Peternakan Vol 2 No 2 (2021): Jurnal Sains dan Teknologi Peternakan
Publisher : Universitas Sulawesi Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31605/jstp.v2i2.960

Fusobacterium necrophorum is a pathogen causing disease in animals, especially cattle, goats, and sheep. F. necrophorum infection can result in a variety of necrotic conditions (necrobacillosis). This study aimed to design a pair of primers for detecting the leukotoxin B (lktB) gene expressed by F. necrophorum as diagnostic support. The lktB gene sequence was obtained from GenBank NCBI with accession number AF312861.3:685-2337. Furthermore, the sequence was used as a template for in silico primer design using Primer-BLAST. Primer candidates successfully designed were then analyzed for their secondary structure using NetPrimer. The results showed that forward primer set 6 (5'-TCGGATGCTGGAATGCTACTT-3') and reverse primer set 6 (5'-GGGCTCCCAAATCCTTACGA-3') were a favorable primer set with a product size of 228 bp. However, laboratory experiments need to be carried out to determine the optimal conditions for this primer set.

Pelatihan Pengolahan Limbah Ampas Nanas Sebagai Produk Pengempuk Daging Reny Rosalina; Aisyah Hadi Ramadani; Riska Surya Ningrum
JPPM (Jurnal Pengabdian dan Pemberdayaan Masyarakat) VOL. 3 NOMOR 2 SEPTEMBER 2019 JPPM (Jurnal Pengabdian dan Pemberdayaan Masyarakat)
Publisher : Lembaga Publikasi Ilmiah dan Penerbitan (LPIP)

Nanas merupakan potensi pertanian yang dihasilkan sangat melimpah tiap tahunnya di Dusun Puhrejo, Kecamatan Ngancar, Kabupaten Kediri. Buah nanas hasil panen selain dijual dalam bentuk buah segar juga diolah menjadi sari nanas. Dari produksi sari nanas dihasilkan limbah ampas nanas yang belum dimanfaatkan. Tujuan dari kegiatan ini adalah untuk meningkatkan pengetahuan tentang pengolahan limbah ampas nanas sebagai pengempuk daging sehingga mendorong kemandirian ekonomi masyarakat melalui pengolahan produk berbasis potensi daerah. Target capaian dalam program ini adalah meningkatkan pengetahuan masyarakat tentang pemanfaatan limbah ampas buah nanas, bertambahnya varian produk olahan buah nanas, serta meningkatnya keinginan masyarakat untuk berwirausaha produk olahan nanas. Metode yang digunakan yaitu melalui penyuluhan dan pelatihan kepada warga Dusun Puhrejo. Hasil kegiatan yaitu pengetahuan masyarakat tentang potensi nanas dan pengolahan limbah ampas nana meningkat, serta keinginan dan kesadaran masyarakat untuk mengolah nanas menjadi produk yang bervariasi meningkat. Hal tersebut juga mendorong masyarakat untuk membentuk kelompok usaha untuk mengembangkan produk olahan berbahan nanas sebagai produk UMKM dusun Puhrejo.

Co-Authors Aisyah Hadi Ramadani Aisyah Ramadani Angella Ananda Saputra Anggita Ratri Pusporini Anita Alni, Anita Desy Yustiyani Didin Mujahidin Dwi Dianita Irawan I Dewa Made Sukrama I Wayan Muda Suta Arta Karin Alifia Rachmadani Lukis, Prima Agusti Ni Nengah Dwi Fatmawati Rian Ka Praja Rio Altha Reyzando Riska Surya Ningrum Rofiatun Solekha Setiyowati, Putri Ayu Ika Siti Zubaidah Sri Bintang Sahara Mahaputra Kusumanegara

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