The Potential of Pasak Bumi as a Food Supplement to Improve Spermatogenesis in Mice Model of Malnutrition

Ida Yuliana (Biomedic Departement, Histology Division, Faculty of Medicine, Lambung Mangkurat University, Banjarmasin, South Kalimantan)
Triawanti Triawanti (Chemsitry and Biochemistry Departement, Faculty of Medicine, Lambung Mangkurat University, Banjarmasin, South Kalimantan)
Didik Dwi Sanyoto (Biomedic Departement, Anatomy Division, Faculty of Medicine, Lambung Mangkurat University, Banjarmasin, South Kalimantan)
Husnul Khatimah (Biomedic Departement, Biology Division, Faculty of Medicine, Lambung Mangkurat University, Banjarmasin, South Kalimantan)
Asnawati Asnawati (Biomedic Departement, Physiology Division, Faculty of Medicine, Lambung Mangkurat University, Banjarmasin, South Kalimantan)
Rayatul Aminah (Medical Education Study Program, Faculty of Medicine, Lambung Mangkurat University Banjarmasin, South Kalimantan)

Jurnal Berkala Kesehatan
Vol 7, No 1 (2021): JURNAL BERKALA KESEHATAN

Malnutrition causes disruption of spermatogenesis in children. Pasak Bumi has the potential to increase spermatogenesis due to malnutrition. Aimed of research is to proved Pasak Bumi as a food supplement to improve spermatogenesis disorders due to malnutrition in malnourished rats. The research method used analytical observational method by observing the histological preparations of the testes of malnourished rats. Observations using a binocular microscope were analyzed with Optilab Camera and Image Raster software. There are 5 groups of experimental: M: malnutrition without intervention; P1: malnutrition + standard feed + PB 7.5 mg/kgBW; P2: malnutrition + standard feed + PB 15 mg/kgBW; P3: malnutrition + standard feed + PB 22.5 mg/kgBW; P4: malnutrition + standard feed + PB 30 mg/kgBW; Research parameters were number of seminiferous tubules, primary spermatocytes and Leydig cells. Data analysis used the Anova One way test with a confidence level of 95%. The results showed number of seminiferous tubules, primary spermatocytes and Leydig cells in the M group was less than the P1-P4 group. This suggests malnutrition causes disruption of spermatogenesis. Conclusion the administration of Pasak Bumi extract at various doses increased spermatogenesis in malnourished.