Pro-Life
Vol. 8 No. 2 (2021): Juli

AKTIVITAS PROTEOLITIK BAKTERI LOKAL YANG DI ISOLASI DARI TEMPAT PENANGKARAN BUAYA

Jendri Mamangkey (Program Studi Pendidikan Biologi, FKIP-Universitas Kristen Indonesia, DKI Jakarta)
Dwi Suryanto (Departemen Biologi, FMIPA-Universitas Sumatera Utara, Medan)



Article Info

Publish Date
19 Jul 2021

Abstract

Indonesia has an ecological diversity which can be used as significantly potential habitat for local bacteria. One of such environments is crocodile breeding where research on proteolytic bacteria is still minimal. Proteolytic bacteria are a type of bacteria which can produce protease enzymes. This research was performed aiming to find selected proteolytic bacteria that can produce protease enzymes. Pour plate method using skim milk agar media was employed to isolate the bacteria. Bacteria isolates were selected based on qualitative and quantitative tests. Current research results discovered 6 bacteria isolates which had proteolytic ability after the qualitative test. Among those 6 isolates, 3 (FB2, THB1b, and THB6a) were able to hydrolyze skim milk media with the fastest incubation time of 18 hours. Meanwhile, based on quantitative test, 1 isolate produced the highest protease activity, which is FB2 isolate with 958 U/mL having a total of 12.05 cells/mL. Based on this research report, FB2 isolate collected from crocodile feces (FB) has the potential to be utilized in protease enzymes application in the environment.

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Journal Info

Abbrev

prolife

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology

Description

Ilmu Biologi merupakan salah satu ilmu yang terus bertumbuh dan menjadi bagian yang penting dalam kehidupan. Ilmu Biologi mencakup kajian teori, penelitian hingga pendidikan Biologi menjadi cakupan ilmiah yang terus diteliti dan dikaji hingga memperoleh manfaat bagi kehidupan. Program studi ...