CHITINOLYTIC BACTERY ACTIVITY ISOLATED FROM THE MUD FIELDS

Nuniek Herdyastuti (Department of Chemistry, Surabaya State University, Jl. Ketintang Surabaya 60231)
Sari Edi Cahyaningrum (Department of Chemistry, Surabaya State University, Jl. Ketintang Surabaya 60231)
Tri Joko Raharjo (Department of Chemistry, Gadjah Mada University, Sekip Utara Yogyakarta, 55281)
Mudasir (Department of Chemistry, Gadjah Mada University, Sekip Utara Yogyakarta, 55281)
Sabirin Matsjeh (Department of Chemistry, Gadjah Mada University, Sekip Utara Yogyakarta, 55281)

Berkala Penelitian Hayati
Vol 15 No 2 (2010): June 2010

A chitinolytic microorganism had been isolated from mud fields in Ketintang Surabaya area by using a minimal media containing 0.4% colloidal chitin. Activity assay is based on released N-acetyl-glucosamine which reacted with 3,5 dinitrosalicyclic acid. Mud fields produce 63 bacterial isolates with chitinase activity (TNH1 – TNH63 isolates). The highest activity was shown by TNH11 isolate with specific activity 1.27 U/mg. TNH11 isolates was Gram negative, rod-cocoid cell, has a colony of yellow, round shape, convex elevation, flat margin and the colony diameter 3–5 mm.