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Mudasir
Department of Chemistry, Gadjah Mada University, Sekip Utara Yogyakarta, 55281
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

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CHITINOLYTIC BACTERY ACTIVITY ISOLATED FROM THE MUD FIELDS Nuniek Herdyastuti; Sari Edi Cahyaningrum; Tri Joko Raharjo; Mudasir; Sabirin Matsjeh
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 15 No 2 (2010): June 2010
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/262

Abstract

A chitinolytic microorganism had been isolated from mud fields in Ketintang Surabaya area by using a minimal media containing 0.4% colloidal chitin. Activity assay is based on released N-acetyl-glucosamine which reacted with 3,5 dinitrosalicyclic acid. Mud fields produce 63 bacterial isolates with chitinase activity (TNH1 – TNH63 isolates). The highest activity was shown by TNH11 isolate with specific activity 1.27 U/mg. TNH11 isolates was Gram negative, rod-cocoid cell, has a colony of yellow, round shape, convex elevation, flat margin and the colony diameter 3–5 mm.
Co-Authors Nuniek Herdyastuti Sabirin Matsjeh Sari Edi Cahyaningrum Tri Joko Raharjo