Antibody against progesterone in local rabbit following low dose of progesterone injection

Suyadi Suyadi (Fakultas Peternakan Universitas Brawijaya)
Sri Wahjuningsih (Department of Animal Production, Faculty of Animal Husbandry, Brawijaya University)
Aulani'am Aulani'am (Department of Biochemisty, Faculty of Mathematics and Sciences, Brawijaya University)
Achadiah Rachmawati (Department of Animal Production, Faculty of Animal Husbandry, Brawijaya University)
P. Srianto (Faculty of Veterinary Medicine, University of Airlangga, Surabaya Indonesia)
M. Pangestu (Faculty of Veterinary Medicine, University of Airlangga, Surabaya Indonesia)

Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science)
Vol 21, No 1 (2011)

ABSTRACT: Antibody against progesgerone was produced from serum of local rabbit following low dose of progesterone injection: While a control group (Control; n=5) was injected with Freund's adjuvant solution in aquadest, the treatment groups were either firstly injected with progesterone conjugated to Freund's Adjuvant (P--CFA, 150 p.l : 150 pl) or progesterone conjugated to Freund's Adjuvant and bovine serum albumin (P-CFA-BSA; 135 p;l : 150 tt1 : 15 gl). Twice boostering injections were adminstered using incomplete Freund's Adjuvant on day 14 and 52 after first immunization. Weekly bleeding for serum collection were done from 1 week following first booster immunization to week 10, Using ELISA technique it was shown that the antibody titer to progesterone after first and second booster immunization in the P-CFA groug was higher than Control- and P-CFA-BSA groups. The antibody titer in the P-CFA-BSA remained low similar in the Control group:Key words: antibody; progesterone; rabbit