Pro-Life
Vol. 8 No. 3 (2021): November

Isolasi Kurkumin dari Kunyit Putih dengan Menggunakan Metode Maserasi dan Kromatografi Lapis Tipis (KLT)

Henni Cintya (Universitas Sumatera Utara)
Michelle Angie Chan (Universitas Sumatera Utara)
Anggreni Purba (Universitas Sumatera Utara)
Tanezsia Kokita (Universitas Sumatera Utara)
Farrencia Destinyie (Universitas Sumatera Utara)
Wilbert Bernardi (Universitas Sumatera Utara)



Article Info

Publish Date
30 Nov 2021

Abstract

Curcumin is an active compound of the polyphenol group found in turmeric. Isolation and identification of curcumin have been done in plants which belong to genus Curcuma, such as Curcuma zedoaria. Curcumin is known for its antitumor, antioxidant properties and has many health benefits. Curcumin have many therapeutic activities, such as antioxidant, anti-inflammatory, and anticarcinogenic. The isolation and identification process was used maceration and TLC (Thin Layer Cromatography) method. The reason using this methods are efficient, easy, simple, and cheap. The extract characterization gave the water content valued at 0,37%, total ash valued at 0,7%, the acid insoluble ash valued at 0,291%. All of these extract characterization adequate the provision. From the results of TLC with a mixture of mobile phase (eluent) i.e. chloroform:ethanol:acetic acid with a ratio of 94:5:1, it shows that there are 3 stains with Rf values, respectively, for curcumin standard Rf1 0.1-0.125; Rf2 0.1375-0.1875; Rf3 0.3375-0.375. As for the white turmeric extract obtained Rf1 0.125-0.225; Rf2 0.1875-0.475; Rf3 0.425-0.675. Meanwhile, the mobile phase mixture (eluent) of chloroform:n-hexane:methanol with a ratio of 1:1:0,1 showed that there were 3 stains with Rf values, respectively, for curcumin the standard was Rf1 0.1-0.125; Rf2 0.1375-0.1875; Rf3 0.3375-0.375. As for the white turmeric extract obtained Rf1 0.1-0.125; Rf2 0.125-0.225; Rf3 0.35-0.375.

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Journal Info

Abbrev

prolife

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology

Description

Ilmu Biologi merupakan salah satu ilmu yang terus bertumbuh dan menjadi bagian yang penting dalam kehidupan. Ilmu Biologi mencakup kajian teori, penelitian hingga pendidikan Biologi menjadi cakupan ilmiah yang terus diteliti dan dikaji hingga memperoleh manfaat bagi kehidupan. Program studi ...