NPC-Exosome Carry Wild and Mutant-type p53 among Nasopharyngeal Cancer Patients

Hamsu Kadriyan (Department of Otolaryngology Head and Neck Surgery, Faculty of Medicine, Universitas Mataram, Jl. Majapahit No.62, Mataram)
Eka Sunarwidhi Prasedya (Centre for Bioscience and Biotechnology, Universitas Mataram, Jl. Majapahit No.62, Mataram)
Nova Audrey Luetta Pieter (Department of Otolaryngology Head and Neck Surgery, Faculty of Medicine, Universitas Hasanuddin, Jl. Perintis Kemerdekaan No.KM.10, Makassar)
Masyita Gaffar (Department of Otolaryngology Head and Neck Surgery, Faculty of Medicine, Universitas Hasanuddin, Jl. Perintis Kemerdekaan No.KM.10, Makassar)
Amsyar Akil (Department of Otolaryngology Head and Neck Surgery, Faculty of Medicine, Universitas Hasanuddin, Jl. Perintis Kemerdekaan No.KM.10, Makassar)
Agusalim Bukhari (Department of Otolaryngology Head and Neck Surgery, Faculty of Medicine, Universitas Airlangga, Jl. Mayjen Prof. Dr. Moestopo No.47, Surabaya)
Budu Budu (Department of Otolaryngology Head and Neck Surgery, Faculty of Medicine, Universitas Airlangga, Jl. Mayjen Prof. Dr. Moestopo No.47, Surabaya)
Andi Alfian Zainuddin (Department of Otolaryngology Head and Neck Surgery, Faculty of Medicine, Universitas Airlangga, Jl. Mayjen Prof. Dr. Moestopo No.47, Surabaya)
Rina Masadah (Department of Otolaryngology Head and Neck Surgery, Faculty of Medicine, Universitas Airlangga, Jl. Mayjen Prof. Dr. Moestopo No.47, Surabaya)
Achmad Chusnu Romdhoni (Postgraduate Program, Faculty of Medicine, Universitas Hasanuddin, Jl. Perintis Kemerdekaan No.KM.10, Makassar)
Abdul Qadar Punagi (Department of Otolaryngology Head and Neck Surgery, Faculty of Medicine, Universitas Hasanuddin, Jl. Perintis Kemerdekaan No.KM.10, Makassar)

The Indonesian Biomedical Journal
Vol 13, No 4 (2021)

BACKGROUND: Nasopharyngeal cancer (NPC) is known to release a specific exosome. NPC-derived exosome (NPC-Exo) could carry p53. However, information regarding the type of p53 carrier on NPC-Exo remains limited. This study aims to introduce our important findings regarding the type of p53 NPC-Exo cargo.METHODS: Serum from patients with NPC were prepared for exosome isolation with Seramir Exoquick by following the manual instructions. RT-PCR was conducted to determine the expression levels of latent membrane protein 1 (LMP-1) and p53 in the exosome isolate. Partial sequencing of p53 amplicon was conducted to determine mutation type of p53.RESULTS: There were 8 patients enrolled in this study. According to RT-PCR results, the expression levels of LMP-1 and p53 varied in the NPC-Exo isolate. Based on sequencing analysis, 1 case of p53 mutation was noticeable.CONCLUSION: According to current results, the NPC-derived exosome potentially carries not only wild type but also mutant type p53. Further research is needed to explore deeper the effect of the mutant type p53 as an exosome carrier in the clinical application.KEYWORDS: Nasopharyngeal cancer, exosome, p53, mutation