Indonesian Journal of Chemistry
Vol 21, No 6 (2021)

Isolation of Ethyl p-Methoxycinnamate from Azadirachta indica Root Bark as Hong Kong Caterpillar (Tenebrio molitor) Antifeedant

Rosnani Nasution (Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia)
Cut Nyak Candein (Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia)
Nurdin Saidi (Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia)
Muhammad Bahi (Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia)
Marianne Marianne (Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155, Indonesia)



Article Info

Publish Date
06 Oct 2021

Abstract

This study aims to identify the antifeedant activity of Azadirachta indica A. Juss root bark against Hong Kong caterpillar (Tenebrio molitor). A. indica A. root was macerated in n-hexane, ethyl acetate, and methanol. The investigation of antifeedant activity was conducted by using the no choice leaf disk method. The antifeedant activity (%AI) tests showed that the extracts of n-hexane, ethyl acetate, and methanol with each concentration of 0.5% were 6.71, 71.78, and 40.14%, respectively. The ethyl acetate extract demonstrated the most active antifeedant activity. Ethyl acetate extract was fractionated to obtain five subfractions (A-E). Each subfraction with each concentration of 0.5% showed the %AI of 70.55, 85.29; 67.40, 82.70 and 82.22%, respectively. Furthermore, GC-MS results demonstrated that ethyl acetate extract contained 19 compounds. The main compounds are methyl hexadecanoate and methyl 8-octadecenoate. Further purification of fraction A, which has the highest amount, was then carried out. The obtained isolate, i.e., A32, was determined as ethyl p-methoxycinnamate confirmed by 13C- and 1H-NMR, FTIR, and MS spectra. The presence of ethyl p-methoxycinnamate makes A. indica A. root is potential as T. molitor antifeedant. The implication of these findings is to reference herbal antifeedants and reduce the use of synthetic pesticides.

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Journal Info

Abbrev

ijc

Publisher

Subject

Chemical Engineering, Chemistry & Bioengineering Chemistry

Description

Indonesian Journal of Chemistry is an International, peer-reviewed, open access journal that publishes original research articles, review articles, as well as short communication in all areas of chemistry including applied chemistry. The journal is accredited by The Ministry of Research, Technology ...