Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology
Vol 17, No 3 (2021): SAINTEK PERIKANAN

SUCCESSFUL GENETIC CHARACTERIZATION OF BULLET TUNA (Auxis rochei) USING MICROSATELLITE MARKERS IN KARANGASEM BALI

Maya Agustina (Loka Riset Perikanan Tuna)
Made Pharmawati (Universitas Udayana)
Ketut Junitha (Universitas Udayana)



Article Info

Publish Date
22 Oct 2021

Abstract

Bullet Tuna (Auxis rochei) is classified under the neritic tuna group, which plays an essential role in small-scale fisheries in Karangasem Bali. The increasing catch of bullet tuna indicated its stock probably under threat. Therefore, genetic characterization is often required as the first step before building a conservation program. This study aims to categorize bullet tuna DNA using microsatellite. Of all five loci used, all were high polymorphism-type, with the number of alleles per locus varied between 18-27. Successful PCR (Polymerase Chain Reaction) created relatively high DNA concentration, ranging from 27.050 to 237.05 ng/ul, with a DNA purity level ranging from 2.073 to 2.239. Overall, the stock condition allegedly still in good condition, marked with high genes diversity (Ho=0.367-0.767 and He=0.934-0.966). All loci used can be amplified and well described.

Copyrights © 2021






Journal Info

Abbrev

saintek

Publisher

Subject

Education

Description

SAINTEK PERIKANAN (p-ISSN: 1858-4748 dan e-ISSN: 2549-0885) adalah jurnal ilmiah perikanan yang diterbitkan oleh Jurusan Perikanan Fakultas Perikanan dan Ilmu Kelautan Universitas Diponegoro. Jurnal ini diterbitkan 2 (dua) kali setahun (Februari dan ...