Garuda - Garba Rujukan Digital

p-Index From 2021 - 2026

2.93

P-Index

This Author published in this journals

All Journal HAYATI Journal of Biosciences INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Kajian Bali Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Journal of Tropical Ethnobiology International Journal of Applied Science and Sustainable Development (IJASSD) International Journal of Scientific Multidisciplinary Research (IJSMR) Metamorfosa: Journal of Biological Science Open Access DRIVERset

I Ketut Junitha

Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana

Author-ID : 1918405

Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Engineering Environmental Science Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mechanical Engineering Medicine & Pharmacology Social Sciences

Published : 13 Documents Claim Missing Document

Claim Missing Document

Articles

1 2

Ragam Alel Mikrosatelit DNA Autosom pada Masyarakat Bali Aga Desa Sembiran Kabupaten Buleleng Bali Junitha , I Ketut; Bagus Alit, Ida
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 16, No 1 (2011): February 2011
Publisher : Universitas Atma Jaya Yogyakarta

Berdasarkan data sejarah dan arkeologis, masyarakat Bali sekarang ini merupakan hasil perkembangan sejarah zaman pra-sejarah. Masyarakat Bali kuno yang masih memiliki tradisi zaman pra-sejarah disebut masyarakat Bali Aga atau Bali Mula. Pada umumnya masyarakat Bali Aga menempati daerah pegunungan seperti desa Tenganan, Terunyan, Sembiran dan Sidatapa, sedangkan masarakat Bali lainnya disebut masyarakat bali Dataran yan tinggal di kota-kota dan daerah pantai. Sebanyak delapan penanda genetik mikrosatelit autosom (D2S1338, D3S1358, D5S818, D7S820, D11S1984, D13S317, D16S539 dan D21S11) digunakan untuk menentukan variasi alel yang tersebar pada masyarakat desa Bali Aga Sembiran kabupaten Buleleng Bali untuk kepentingan forensik. Dalam penelitian ini ditemukan sebanyak 46 ragam alel dari 8 lokus yang digunakan, ragam alel perlokus berkisar antara 3 pada lokus D5S818 sampai 9 alel pada lokus D11S1984. Nilai kemampuan pembeda (power of discrimination/PD) tertinggi ditemukan pada lokus D11S1984 (0,9394) diikuti oleh D21S11(0,8922), D16S539 (0,8915), D13S317 (0,8602), D7S820 (0,8398), D3S1358 (0,8014), D2S1338 (0,5518) dan D5S818 (0,0143). Hasil ini menunjukkan bahwa lokus D5S818 tidak baik digunakan dalam analisis DNA untuk kepentingan forensik pada masyarakat Bali Aga desa Sembiran.

MUTATIONS IN 1700 BP FRAGMENT OF RPOB GENE OF MULTI-DRUG RESISTANT MYCOBACTERIUM TUBERCULOSIS ISOLATE Yowani, Chandra; Sukardika, I K.; Mantik-Astawa, I N.; Junitha, I K.
INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Vol 6 No 2 (2012): Indonesian Journal of Biomedical Sciences
Publisher : Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (474.737 KB)

This research aimed to amplify a 1700 bp fragment of rpoB gene of multidrug resistance M. tuberculosis (MDR-TB) isolate and determine types of mutation beyond the core region (hot-spot region). DNA sequencing studies indicate that more than 95% of rifampin-resistant M. tuberculosis strains have mutations within the 81-bp hot-spot region (codons 507 to 533) of the RNA polymerase ?-subunit (rpoB). Since almost 90 % of rifampicin resistant isolate are also resistant to isoniazid, mutation in rpoB gene become important as a surrogate marker for MDR-TB. MDR- TB isolates used for this research, namely isolate 885, was collected by Regional Health Laboratory of Surabaya. PCR was used to amplify the gene, on described steps : a cycle of preheating at 95°C for 15 minutes, amplifying in 45 cycles ( 1 minute at 94°C, 1 minute at 58°C, 1 minute 72°C) and post extension for 5 minutes at 72°C. The mutations were detected by sequencing and alignment using MEGA4. The result of this research showed that there were new mutations downstream of the core region of rpoB. Sequence analysis showed some mutations such as S594A, S626V, T629A. In conclusion, it is reported for the first time, new mutations at downstream region of the core region of rpoB.

Y-Chromosome Microsatellite DNA Variations on Terunyan Bali Mula Society I KETUT JUNITHA; SANG KETUT SUDIRGA
HAYATI Journal of Biosciences Vol. 14 No. 2 (2007): June 2007
Publisher : Bogor Agricultural University, Indonesia

The Balinese is comprised of genealogical patrilineal clans named as soroh. However, remnants of ancient Bali Mula communities have their own local genealogy which differ from the general soroh. The Pasek Trunyan together with Pasek Kayu Selem, Pasek Celagi, and Pasek Kayuan are part of Bali Mula which believed to be descendants of one ancestor, Empu Kamareka. Here we reported genetic variation of the Terunyan society. The microsatellites Y-chromosome markers (DYS19, DYS390, DYS393, and DYS395) were used in this study. There were 12 alleles found and the result showed the low genetic diversity (D = 0.28 + 0.05) within Terunyan society. Nine haplotypes based on allelic combination were found, dominated by major haplotype with frequency of 0.50 which spread out in many genealogical lineage called dadia. The haplotype data showed that Terunyan society derived from many gene sources. Key words: patrilineal, pasek, soroh, dadia, haplotipe

SUCCESSFUL GENETIC CHARACTERIZATION OF BULLET TUNA (Auxis rochei) USING MICROSATELLITE MARKERS IN KARANGASEM BALI Maya Agustina; Made Pharmawati; Ketut Junitha
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 17, No 3 (2021): SAINTEK PERIKANAN
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijfst.17.3.%p

Bullet Tuna (Auxis rochei) is classified under the neritic tuna group, which plays an essential role in small-scale fisheries in Karangasem Bali. The increasing catch of bullet tuna indicated its stock probably under threat. Therefore, genetic characterization is often required as the first step before building a conservation program. This study aims to categorize bullet tuna DNA using microsatellite. Of all five loci used, all were high polymorphism-type, with the number of alleles per locus varied between 18-27. Successful PCR (Polymerase Chain Reaction) created relatively high DNA concentration, ranging from 27.050 to 237.05 ng/ul, with a DNA purity level ranging from 2.073 to 2.239. Overall, the stock condition allegedly still in good condition, marked with high genes diversity (Ho=0.367-0.767 and He=0.934-0.966). All loci used can be amplified and well described.

Genetic Diversity and Population Structure of Bullet Tuna (Auxis rochei) from Bali and Its Adjacent Waters Maya Agustina; Bram Setyadji; Made Pharmawati; I ketut Junitha
HAYATI Journal of Biosciences Vol. 29 No. 4 (2022): July 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.4.507-514

Bullet tuna (Auxis rochei) dominates the neritic tuna catch, especially from the purse seine fleet within the western and southern Indonesian waters. However, high catches can lead to stock depletion and lower genetic diversity due to possible inbreeding. Therefore, population genetic information is important in monitoring the sustainability of fish stocks and proposing an appropriate species-specific conservation strategy. This study aimed to analyze the genetic diversity, population structure, and kinship relationship of bullet tuna in Bali and its adjacent waters. Sampling was carried out in September 2020 at landing sites/ports representing the north, east, south, and west region, whereas at least 30 samples were acquired at each location. The result showed that the DNA concentration obtained could produce DNA bands with allele length ranged from 94-260 bp. Observed heterozygosity (Ho) was around 0.440-0.627. While the expected heterozygosity (He) was between 0.932-0.945. The genetic variation among population, within-population, and individuals was 0.36%, 41.04%, and 58.60%, respectively. The results of the analysis of genetic diversity between individuals in the population showed very high genetic diversity. The population structure of the bullet tuna landed in West Bali, East Bali, South Bali and North Bali is the same population stock. The kinship relationship indicates that the four populations are closely related genetically.

Ragam Alel Mikrosatelit DNA Autosom pada Masyarakat Bali Aga Desa Sembiran Kabupaten Buleleng Bali I Ketut Junitha; Ida Bagus Alit
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 16, No 1 (2011): February 2011
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v16i1.60

Profil genetika DNA mikrosatelit kromosom-Y masyarakat laki-laki soroh Kayuan Pasek Catur Sanak Bali Mula I Ketut Junitha; Ni Luh Watiniasih; Ni Luh Putu Ria Puspitha
Jurnal Kajian Bali (Journal of Bali Studies) Vol 7 No 1 (2017): RELASI ETNISITAS DI BALI
Publisher : Pusat Kajian Bali Universitas Udayana

This research is conducted to find out the genetic profiles of Kayuan clan which is belong to Pasek Catur Sanak Bali Mula, indigenous Balinese people. The DNA profiles is identified by using Microsatellite DNA of Y chromosome. Genes or DNA in Y chromosome are descended from father to son (patrilinealism). Four loci of microsatellite DNA from Y chromosome are used, including DYS19, DYS390, DYS393 and DYS395 to amplified DNA samples from 67 males probands. The probands are people of Kayuan clan from Siakin, Songan, Blandingan and Kutuh villages, Kintamani sub district and others are originally from Jehem village, Tembuku sub district, Bangli regency. The research found 12 allele varieties from all the analyzed loci, which resulted in low genetic diversity (0.33 + 0.001). There are 4 combinations alleles of 4 loci that created12 various haplotypes, the greatest one is haplotype 1 (0.39), followed by haplotype 2 (0.16), haplotype 7 (0,12) and the smallest one is haplotype 6 (0,10). On the other hand, eight other alleles found on the smaller frequencies. Two similar allele combinations were also found on DNA profiles of Celagi clan, which is also belong to the similar family, Pasek Catur Sanak Bali Mula. The two allele are haplotype 6 and 5 found in both, Pasek Kayuan and Pasek Celagi. The haplotype 6 as dominant allele of Celagi clan by adoption, haplotype 5 however, the frequency was the lowest. This allele only detected in one member of each clan due to mutation.

Penanda DNA mikrosatelit Kromosom-Y untuk Penelusuran Soroh-soroh/Kawitan Masyarakat Bali I Ketut Junitha
Journal of Tropical Ethnobiology 2021: Prosiding Seminar Nasional PMEI V 2020
Publisher : The Ethnobiological Society of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar

Soroh is a Balinese term for a clan in Indonesian. Each soroh is a group of Balinese Hindus who believe they are descended from one common ancestor, and has one main temple as a place of worship to the ancestors, which is called the kawitan temple. Each main soroh can be divided into several sub-soroh based on the male lineage of the main ancestor. Each sub-soroh also has a temple for their ancestors’ worship which is also called pura kawitan. Therefore, there are known subsoroh and pura kawitan main temples, whose members are a combination of sub-soroh and sub-sections. Soroh Catur Sanak Bali Mula believes he is a descendant of Mpu Kamereka which developed into four sub-soroh, namely Kayu Selem, Celagi, Kayuan, and Terunyan. The family system in Bali adheres to a patrilineal descent where all children from one family will follow the cheer of their father. For Balinese Hindus, knowing their soroh or kawitan temple is an obligation to be able to offer prayers to their kawitan temple as a form of respect for each offspring to their ancestors. Losing traces of ancestors (kawitan) is believed to be a major problem in the family life. Based on the belief that each soroh is descended from one ancestor and the family system in Bali adheres to a patrilineal lineage, soroh or kawitan tracing can be carried out by DNA analysis with Ychromosome microsatellite markers. DNA or genes on the Y chromosome that are only present in males will be passed from males to only males and so on. Therefore, the Y-chromosome microsatellite profile can be used as a search tool for Soroh or Kawitan in Balinese society.

Hubungan Genetika Pria Masyarakat Sembiran dan Tri Wangsa di Bali I Ketut Junitha; Bambang Suryobroto
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 27, No 1 (2010)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2010.27.1.185

Balinese community has evolved since the prehistoric era. The present community is structured into Hinduism caste (Brahmana, Kesatria, Wesia and Sudra), but remnants of their ancient communities, collectively called Bali Aga, have not undergone this structure. The first three castes of modern Balinese that comprises Tri Wangsa gentry were derived from JavanesekingdomofMajapahit(1343 AD). One of Bali Aga communities is Sembiran of Northern Bali. Using chromosomal microsatellite DNA, allelic variability of Sembiran and Tri Wangsa communities was reported. Both of them had the same high frequency of alleles which suggested genetic intermixing or sharing of common ancestor. However, a few alleles were unique for each community. This uniqueness was related to patrilineal system of Balinese community and to high mutation rate of Y chromosomal microsatellite DNA.

Ethnobotany of Medicinal Plant Diversity as A Traditional Medicine in Bugbug Karangasem, Bali, Indonesia Dewa Ayu Sri Ratnani; I Ketut Junitha
International Journal of Scientific Multidisciplinary Research Vol. 3 No. 1 (2025): January 2025
Publisher : PT FORMOSA CENDEKIA GLOBAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55927/ijsmr.v3i1.12852

Traditions and cultures used as tourism assets can ignore traditional practices with a concurrent loss of traditional knowledge. This study aims to reveal the traditional knowledge of medicinal plants used by the Bugbug community through analysis of the traditional knowledge of the diversity of medicinal plants, the fidelity level (FL) and Index of Cultural Significance (ICS) of medicinal plants. The study was conducted in Bugbug Village, Karangasem, from May to July 2022. Data were collected using qualitative methods through semi-structured interviews, moderate participation observation, and documentation. Key informants and respondents were selected using purposive and snowball sampling; 10 informants and 48 respondents were obtained. The fidelity level was calculated by FL; and cultural importance by ICS. The level of traditional knowledge of medicinal plants was measured by the Phillips and Gentry equations and analyzed by the Kruskal Wallis and Mann Whitney Test. As many as 124 species in 46 families are found at Bugbug Village. The best represented families were Fabaceae. Leaves are the most widely used plant parts for traditional medicine. The highest habitat is obtained from the roadsides. In terms of the life form, most of the used species were herbaceous. Traditional knowledge between age groups differs significantly. In contrast, traditional knowledge of gender was not found to be different. The highest fidelity levels are Piper betle L. and Imperata cylindrica L. And the most ICS is Piper betle L

Co-Authors A.A. Gde Lanang Adelina Arifiani Purnomo Agriani Dewinta Anastesya Hartika Nur Saputri Ayu Saka Laksmita W Bagas Susilo Bagus Alit, Ida Bambang Suryobroto Bram Setyadji Budiningsih, Desak Nyoman Cakra Diarsa Chandra Yowani Christian Subagya Gunardi Dewa Ayu Sri Ratnani Dwi Ariani Yulihastuti ENIEK KRISWIYANTI Eswaryanthi Kusuma Yuni Fita Efi Rafelina Siallagan Graciella Stevani Gulo Gusti Ayu Putri Arnila Hanas, Dicky Frengky I Gde Suryadi I Gede Yeyen Suharta I Gusti Agung Eka Saraswati I K. Sukardika I Ketut Sundra I Made Sara Wijana I NYOMAN MANTIK ASTAWA I Wayan Arya Mahardika Ida Ayu Manik Damayanti Ida Bagus Alit Ida Bagus Bajing Agastya Ida Bagus Made Suaskara IMA Gelgel Wirasutha Inna Narayani IRIANI SETYAWATI Kezia Artanauli Purba Lucia Emy Octavia MADE PHARMAWATI Maya Agustina Maya Agustina Meitini Proborini Wahyuni Muhamad Masyrur NI KADEK YUNITA SARI Ni Luh Putu Ria Puspitha Ni Luh Putu Rika Sugiantari Ni Luh Watiniasih Ni Made Puspawati Ni Made Rai Suarni Ni Made Suartini Ni Made Suartini Ni Nyoman Wirasiti Ni Putu Puniari Eka Putri, Ni Putu Puniari Eka Ni Putu Putri Wulandari Putri Arie Prasetyoningrum Putri Karenina Siregar Putu Rosi Setyari SANG KETUT SUDIRGA Septiasari, Ni Putu Senshi Tyara Tyara Victor Peter Poli Yan Ramona Yossy Carolina Carolina YOSSY CAROLINA UNADI Yulia Mirayanti

Title

Found 13 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 13 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 13 Documents
Search