Activity of Trigonella foenum-graecum on Some Cell Lines.

Agustini, Kurnia ( Center for Pharmaceuticals and Medical Technology Laboratory for Development of Industrial Agro and Biomedical Technology Agency for the Assessment and Application of Technology ( BPPT))
Sumaryono, Wahono ( Center for Pharmaceuticals and Medical Technology Laboratory for Development of Industrial Agro and Biomedical Technology Agency for the Assessment and Application of Technology ( BPPT))
Widyanto, R Micho ( Center for Pharmaceuticals and Medical Technology Laboratory for Development of Industrial Agro and Biomedical Technology Agency for the Assessment and Application of Technology ( BPPT))

Indonesian Journal of Cancer Chemoprevention
Vol 2, No 2 (2011)

Trigonella  foenum-graecum  (TFG)  is  one  of  medicinal  plants  contains  some steroidal sapogenin  such as diosgenin, yamogenin, gitogenin, tigogenin and trigoneoside, also  alkaloid  trigonellin,  which  is  have  many  activity  as  antidiabetic,  estrogenic  and  also as  anti  cancer.    This  experiment  was  done  to  explore  the  activity  of  some  extract  of TFG on some cell lines such as MCF7 (Human Breast Cancer Cell-line), T47D (Human Breast  Cancer  Cell-line),  PC3  (Human  Prostate  Cell-line)  and  SKOV  (Human  Ovarian Carcinoma Cell-line).  This assay was done using MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium)  methods.    Results  showed  that  ethyl  acetate  fraction  gives  the lowest  IC50  than  another  extracts.  IC50  for  PC3  is  66.24  ppm,  IC50  for  MCF7  is  41.81 ppm,  IC50  for  T47D  is  58.63  ppm.    These  datas  can  be  used  for  further  research  to isolate the active compound from TFG. Keywords: Trigonella foenum-graecum, MCF-7, T47D, SKOV, PC3.