PRIMER OPTIMIZATION AS GENETIC MARKER PRIMER BASED ON CYTOCHROME B GENE ON SUMATRAN ELEPHANT (Elephas maximus sumatranus) NON-INVASIVE SAMPLES

Eko Agus Srihanto (Lampung Disease Investigation Center)
Elly Lestari Rustiati (Biology Department, Faculty of Mathematics and Natural Sciences, University of Lampung)
Priyambodo Priyambodo (Biology Department)
Dian Neli Pratiwi (Magister of Environmental Sciences, University of Lampung)
Alvin Wiwiet Susanto (Biology Department, Faculty of Mathematics and Natural Sciences, University of Lampung)
Diah Esti Anggraini (Prof. Dr. Ir. Rubini Atmawidjaja Elephant Hospital, Way Kambas National Park)
Enny Saswiyanti (Lampung Disease Investigation Center)

BIOVALENTIA: Biological Research Journal
Vol. 8 No. 1 (2022)

Sumatran elephant is declared as critically endangered by IUCN since 2011. In supporting conservation efforts on sumatran elephant in its natural habitat, molecular data collection of their population needs to be done. Genetic source material is mostly obtained in its natural habitat is its dung/feces. Study on cytochrome B gene as genetic marker from sumatran elephant fecal samples can be done to get additional genetic data as a form of conservation effort. Primer optimization as genetic marker based on cytochrome B gene has been carried out as an early study on sumatran elephant genetics.  Primer optimization program based on cytochrome B genetic marker from sumatran elephant fecal samples was under Higher Education Applied Research funded by The Ministry of Research, Technology, and Higher Education. DNA was isolated from fecal samples of captive sumatran elephants in Elephant Training Center, Way Kambas National Park. Primer optimization was done via amplification by increasing certain temperature. Specificity test was done to ensure that the primer only recognized sumatran elephant DNA. The best result was obtained with the annealing temperature of 52oC