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All Journal Bioma : Berkala Ilmiah Biologi Biotika: Jurnal Ilmiah Biologi El-Hayah : Jurnal Biologi Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Pengabdian Kepada Masyarakat Sakai Sambayan Jurnal Penelitian Sains Jurnal Biologi Tropis Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Jurnal Biogenerasi Jurnal Ekologi, Masyarakat dan Sains Journal of Community Service and Empowerment Jurnal Pengabdian Kepada Masyarakat (JPKM) Tabikpun Mattawang: Jurnal Pengabdian Masyarakat Jurnal Pengabdian Dharma Wacana BIOVALENTIA: Biological Research Journal Jurnal Mitrawarga JURNAL BIOLOGI PAPUA The International Journal of Tropical Veterinary and Biomedical Research Journal of Community Service (Abdisci) BIO-SAINS | Jurnal Ilmiah Biologi
Priyambodo Priyambodo
Fakultas Matematika Dan Ilmu Pengetahuan Alam Universitas Lampung
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THE ESSENTIAL CONTRIBUTION OF CAPTIVE SUMATRAN ELEPHANT IN ELEPHANT TRAINING CENTER, WAY KAMBAS NATIONAL PARK FOR WILDLIFE GENETICS CONSERVATION Rustiati, Elly Lestari; Priyambodo, Priyambodo; Yulianti, Yanti; Srihanto, Eko Agus; Pratiwi, Dian Neli; Virnarenata, Elsa; Novianasari, Tika; Krismuniarti, Elisabeth Devi; Saswiyanti, Enny
BIOVALENTIA: Biological Research Journal Vol. 6 No. 1 (2020)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1061.173 KB) | DOI: 10.24233/BIOV.6.1.2020.173

Abstract

Way Kambas National Park (WKNP) is home of five protected big mammals including sumatran elephants.  It shares its border with 22 of 37 villages surrounding the national park.  Understanding their existence in the wild is a priority, and  wildlife genetics is a crucially needed. Besides poaching and habitat fragmentation, wildlife-human conflict is one big issue.  Elephant Training Center (ETC) in WKNP is built for semi in-situ conservation effort on captive sumatran elephants that mainly have conflict histories with local people.  Participative observation and bio-molecular analysis were conducted to learn the importance of captive Sumatran elephant for conservation effort.  Through captive sumatran elephants, database and applicable methods are expected to be developed supporting the conservation of their population in the wild.  Participative observation and molecular identification was carried on captive sumatran elephants in ETC, WKNP under multiple year Terapan grant of Ministry of Research and Technology Higher Education, Indonesia. Gene sequence and cytological analyses showed that the captive sumatran elephants are closely related and tend to be domesticated.  Translocation among ETC to avoid inbreeding, and maintaining the captive sumatran elephant as natural as possible are highly recommended. Developing genetic database can be a reference for both captive and wild sumatran elephants.
IDENTIFICATION AND CHARACTERISATION OF COI GENE IN FEMALE SUMATRAN ELEPHANT (Elephas maximus sumatranus) IN ELEPHANT TRAINING CENTRE, WAY KAMBAS NATIONAL PARK Virnarenata, Elsa; Rustiati, Elly Lestari; Priyambodo, Priyambodo; Srihanto, Eko Agus; Pratiwi, Dian Neli
BIOVALENTIA: Biological Research Journal Vol. 7 No. 1 (2021)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (716.49 KB) | DOI: 10.24233/biov.7.1.2021.175

Abstract

Sumatran elephant is a subspecies of endemic Asian elephants on the island of Sumatra and is included in the Red List of the International Union for Conservation of Nature (IUCN) with critically endangered status. The building of the Elephant Training Centre (ETC) in Way Kambas National Park (WKNP) is one of the conservation efforts of Sumatran elephants. Small and closed population size lead to an increased risk of inbreeding that triggers reduction in genetic variation and viability and increases the risk of extinction. The phylogenetic pattern of Sumatran elephants in Indonesia has shown a low population genetic diversity. Genetic diversity information is indispensable to support the direction of decision making in Sumatran elephant conservation policy. The DNA isolation of Sumatran elephants in ETC, WKNP has performed as a first step to trace its genetic variation. The advanced step of DNA isolation is the use of cytochrome oxidase subunit I (COI) gene for identification of genetic characteristics in Sumatran elephants. The COI gene is one of the genes on the mitochondrial genome and in molecular studies it is used as a genetic marker to study genetic characteristics between species and individuals. Identification and characterisation are done by sequencing process and data analysis in the form of electroforegram using Molecular Evolution Genetics Analysis (MEGA) software version 6.0. to see the genetic diversity of the female Sumatran elephant population in ETC, WKNP. Based on the results of the analysis it is indicated that the genetic distance of 24 individual female Sumatran elephant from PLG, TNWK is 0.000 with a homology value of 100%, strengthened by the construction of phylogenetic tree. The absence of genetic distance indicates a close genetic relationship, so it can be concluded all individual female Sumatran elephants in the PLG, TNWK is derived from one population group.
Herpesviridae identification method on Ruminants through Molecular Method in Lampung Disease Investigation Center Alvin Wiwiet Susanto; Elly Lestari Rustiati; Priyambodo Priyambodo; Yuni Tinasari; Firwantoni Firwantoni; Romaya Wulan Suciningtyas; Eko Agus Srihanto; Enny Saswiyanti
Jurnal Penelitian Sains Vol 22, No 2 (2020)
Publisher : Faculty of Mathtmatics and Natural Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (282.55 KB) | DOI: 10.56064/jps.v22i2.578

Abstract

Farming is one sector that has an important role in building Indonesia's economic growth, especially for daily food. Farm production is facing challenges, include the low production yield, traditional management, and problems related to animal health. Infectious Bovine Rhinotracheitis (IBR) and Malignant Catarrhal Fever (MCF) are diseases caused by Herpesviridae. These are high mortality rate disease cause huge economic loss. Farms located nearby deer captivity increases the risk of deer to be infected by the same diseases. Surveillance is needed to discover the number of infected animals and their effect on the environment. Herpesviridae identification in surveillance was done through an accurate method, e.g. a molecular method. This research is done by Lampung Disease Investigation Center and funded by the Food Agriculture Organization (FAO). Herpesviridae identification was done in three steps, DNA isolation, Polymerase Chain Reaction (PCR), electrophoresis, and visualization.
The Effect of Cocopeat and Charcoal Combination in Growing Media of Green Mustard (Brassica rapa L.) Var. Parachinensis Growth Masniar Vina Kesti; Bambang Irawan; Priyambodo Priyambodo; Martha Lulus Lande
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 7 No. 1 (2020)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v7i1.18

Abstract

Green mustard is one type of vegetable that is easily cultivated and has good prospects to increase farmers' income and community nutrition. Growing media is a material that is useful as a place to stand upright seedlings, to develop seedling roots, to store water, gas, and nutrients needed for seedling growth. The purpose of this study is a). to determine the effect of using a combination of cocopeat and charcoal as a medium for planting mustard greens, b). to get the best dosage of the composition of the growing medium on the growth of mustard greens. The study was conducted in a completely randomized design (CRD) with 4 treatments and 6 replications. The data were analyzed by analysis of variance (ANOVA) and further tested with the Least Significant Difference test (LSD) = 5%. The results showed that the use of cocopeat growing media and charcoal influenced germination and vigor index. Cocopeat and charcoal have a significant effect on fresh and dry weight and chlorophyll content of mustard greens. The mixture of cocopeat growing media and charcoal on P1 did not affect the growth of green mustard on the dry weight. Fresh weight gave a significant effect on K1 and P1 was also significantly different from P2 and P3.
SINERGITAS PENGGIATAN EKONOMI KERAJINAN BATIK LAMPUNG, EKSPLORASI BUDAYA DAN EDUKASI KONSERVASI: ANDANAN BATIK TULIS, NEGERI SAKTI, PESAWARAN, LAMPUNG Elly Lestari Rusitati; Erdi Suroso; Warsono Warsono; Junaidi Junaidi; Favorisen Rosyking Lumbanraja; Priyambodo Priyambodo
Jurnal Pengabdian Kepada Masyarakat Sakai Sambayan Vol 3 No 2 (2019)
Publisher : Lembaga Penelitian dan Pengabdian Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jss.v3i2.146

Abstract

Batik Lampung merupakan salah budaya yang khas dengan motifnya dan menjadi salah satu kegiatan ekonomi yang kuat di Provinsi Lampung, termasuk kerajinan Andanan Batik Tulis, yang mencirikan keunikan budaya Lampung. Saat ini selain sebagai kegiatan kebutuhan ekonomi, kerajinan Andanan Batik Tulis sekaligus menjadi media edukasi budaya dan konservasi melalui pengkayaan motif berbasis budaya Lampung, konservasi dan penyediaan media belajar membatik. Penerapan edukasi budaya dan konservasi dilakukan melalui paket eduwisata yang disediakan bagi wisatawan baik lokal maupun asing. Program eduwisata ini mendapat tanggapan positif. Kata Kunci: Andanan Batik Tulis, eduwisata, budaya, konservasi, Lampung
Pengolahan Sampah Plastik Menjadi Paving Block di Desa Margasari, Lampung Timur Melya Riniarti; Winda Rahmawati; Priyambodo Priyambodo; Tristiyanto Tristiyanto; Patrice Kevin Marcus; Putri Ayu Febrina; Eva Yunita
Jurnal Pengabdian Kepada Masyarakat (JPKM) TABIKPUN Vol. 3 No. 1 (2022)
Publisher : Faculty of Mathematics and Natural Sciences - Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jpkmt.v3i1.76

Abstract

Kebiasan masyarakat membuang sampah ke badan air menjadi masalah serius karena sampah terakumulasi di hutan mangrove. Sampah plastik dapat menyebabkan kematian tanaman mangrove dan mengancam kelestarian hutan mangrove di Desa Margasari. Inovasi yang ditawarkan dalam kegiatan pengabdian kepada masyarakat ini adalah eco paving block. Produk ini tidak rumit pembuatannya dan membutuhkan sampah plastik dalam jumlah besar. Tujuan kegiatan ini adalah (1) meningkatkan pengetahuan dan keterampilan masyarakat dalam mengolah dan memanfaatkan sampah plastik dan (2) meningkatkan daya tarik eduwisata Lampung Mangrove Centre. Kegiatan diawali dengan penyuluhan dilanjutkan dengan pelatihan pembuatan paving block. Hasil evaluasi 20 orang peserta menunjukkan bahwa terjadi peningkatan pengetahuan tentang bahaya plastik bagi ekosistem mangrove dan peserta mampu membuat paving block dengan baik. Peserta sangat antusias dan berharap kegiatan ini dapat diduplikasi kepada lebih banyak warga masyarakat.
Klorofil Sebagai Indikator Tingkat Toleransi Kekeringan Kecambah Padi Gogo Varietas Lokal Lampung, Lumbung Sewu Cantik Aprilia Eka Putri; Eti Ernawiati; Priyambodo Priyambodo; Rochmah Agustrina; Lili Chrisnawati
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 7, No 2 (2022): June 2022
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v7i2.5150

Abstract

Kekeringan merupakan salah satu tantangan global saat ini. Hal tersebut dihadapi juga pada budidaya padi di Indonesia. Penemuan plasma nutfah padi toleran kekeringan diperlukan untuk pengembangan varietas baru di masa mendatang. Tujuan penelitian ini adalah untuk mengetahui respon adaptasi padi lokal Lampung, Lumbung Sewu Cantik (LSC) di bawah cekaman kekeringan melalui pengukuran kadar klorofil pada tahap perkecambahan. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan dua faktor, yaitu varietas padi Lumbung Sewu Cantik (LSC), INPAGO 8 (K+), dan IR64 (K-); dan konsentrasi larutan PEG 6000, diantaranya 0% (kontrol) dan 20%. Kecambah ditumbuhkan dengan metode Uji Kertas Digulung dalam plastik (UKDdp). Parameter yang diamati yaitu kadar klorofil a, klorofil b, dan klorofil total. Data yang diperoleh dianalisis dengan ANOVA dan uji lanjut DMRT pada taraf α=0,05. Hasil penelitian menunjukkan bahwa perlakuan konsentrasi PEG 6000 dan varietas padi berpengaruh signifikan terhadap kandungan klorofil a, b dan total. Perlakuan cekaman kekeringan menyebabkan penurunan kadar klorofil kecambah padi secara signifikan. Varietas LSC menunjukkan respon yang sama terhadap cekaman kekeringan dengan varietas rentan (IR64), dan berbeda dengan varietas toleran (INPAGO 8). Dengan demikian, varietas LSC pada karakter klorofil kecambah tidak menunjukkan ketahanan terhadap kekeringan.
Inisiasi Tata Kelola Kawasan Gapoktanhut Lestari Sejahtera, Sedayu-Tanggamus Elly Lestari Rustiati; I Gede Swibawa; Jani Master; Intan Diani Fardinatri; Bondan Pergola; Dian Neli Pratiwi; Priyambodo
Jurnal Pengabdian Kepada Masyarakat (JPKM) TABIKPUN Vol. 3 No. 2 (2022)
Publisher : Faculty of Mathematics and Natural Sciences - Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jpkmt.v3i2.80

Abstract

Gabungan Kelompok Tani dan Hutan (Gapoktanhut) Lestari Sejahtera, Pekon Sedayu, Kecamatan Semaka, Kabupaten Tanggamus, Provinsi Lampung telah diberikan izin Perhutanan Sosial untuk rencana pengelolaan yang berkelanjutan. Secara kolektif Gapoktanhut Lestari Sejahtera dapat melakukan pengelolaan, pengembangan dan pemanfaatan hutan, dengan kewajiban tata kelola kawasan untuk melindungi keanekaragaman hayati. Tantangan yang dihadapi adalah pemahaman nilai keanekaragaman hayati dan sumber daya yang terbatas. Universitas Lampung bersama dengan Rainforest Alliance and KPH Kotaagung Utara, melakukan pendampingan inisiasi tata kelola kawasan Gapoktanhut Lestari Sejahtera untuk penggalian potensi alam dan meningkatkan kemampuan kelola gapoktanhut melalui diskusi kelompok dan survei penelusuran cepat keanekaragaman hayati partisipatif. Pelibatan gapoktanhut penting karena aktivitas petani bersinggungan dengan hutan lindung. Penelusuran cepat partisipatif tentang pengenalan GPS, Kobo Tool Box, tumbuhan dan hewan, secara langsung dan tidak langsung, pembuatan herbarium sangat bermanfaat sebagai dasar pendekatan untuk perlindungan, pengelolaan dan pemanfaatan berkelanjutan keanekaragaman hayati dan pada akhirnya untuk pengawetan habitat hidupan liar.
PRIMER OPTIMIZATION AS GENETIC MARKER PRIMER BASED ON CYTOCHROME B GENE ON SUMATRAN ELEPHANT (Elephas maximus sumatranus) NON-INVASIVE SAMPLES Eko Agus Srihanto; Elly Lestari Rustiati; Priyambodo Priyambodo; Dian Neli Pratiwi; Alvin Wiwiet Susanto; Diah Esti Anggraini; Enny Saswiyanti
BIOVALENTIA: Biological Research Journal Vol. 8 No. 1 (2022)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (773.02 KB) | DOI: 10.24233/biov.8.1.2022.216

Abstract

Sumatran elephant is declared as critically endangered by IUCN since 2011. In supporting conservation efforts on sumatran elephant in its natural habitat, molecular data collection of their population needs to be done. Genetic source material is mostly obtained in its natural habitat is its dung/feces. Study on cytochrome B gene as genetic marker from sumatran elephant fecal samples can be done to get additional genetic data as a form of conservation effort. Primer optimization as genetic marker based on cytochrome B gene has been carried out as an early study on sumatran elephant genetics.  Primer optimization program based on cytochrome B genetic marker from sumatran elephant fecal samples was under Higher Education Applied Research funded by The Ministry of Research, Technology, and Higher Education. DNA was isolated from fecal samples of captive sumatran elephants in Elephant Training Center, Way Kambas National Park. Primer optimization was done via amplification by increasing certain temperature. Specificity test was done to ensure that the primer only recognized sumatran elephant DNA. The best result was obtained with the annealing temperature of 52oC
Pelatihan Budidaya Lebah tanpa Sengat di Desa Harapan Jaya Kecamatan Way Ratai Kabupaten Pesawaran Mahfud Sidik; Indah Ayu Lestari; Lousanja Dira Sa’uddah; Ani Andri Yani; Priyambodo Priyambodo
Jurnal Mitrawarga Vol. 1 No. 1 (2022): Jurnal Mitrawarga
Publisher : Fakultas Ilmu Sosial dan ilmu Politik

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (517.51 KB) | DOI: 10.23960/jmw.v1i1.9

Abstract

Harapan Jaya Village, Way Ratai District, Pesawaran Regency is a village located at an altitude of 400 to 500 meters above sea level (masl), precisely on the southern slopes of Mount Pesawaran and directly adjacent to the protected block area of ​​Register 19 Forest. The majority of the population in this village are from the lower middle class and have quite difficult access from the center of the economy because of its location at an altitude. This training aims to increase the understanding of villagers about stingless beekeeping. The training began with a survey in February 2022 and followed up in May and June 2022. This training activity is an integrated part of the implementation of the Community Service Student Creativity Program (PKM-PM). The training was carried out at the Harapan Jaya Village Office and one of the residents' houses where logs beeThe method of implementing the training is through lectures, audio-visual media playback, questions and answers, and practice. Residents are trained to make bee logs, and choose a location for log placement of Trigona itama. An increase in public understanding is expected to have an impact on improving the people's economy, especially in the post-pandemic era.
Co-Authors Agustin, Meli Agustrina, Rochmah Ahyul Heni Alvin Wiwiet Susanto Alvin Wiwiet Susanto Amaliya Sabana Ani A. Yani Ani Andri Yani Ani Andri Yani Aprilia Eka Putri Arsan, Zulfi Aspita Laila Ayuni, Ubaid Jan Bambang Irawan Bondan Pergola Chicka Refina P. Putri Danisworo Zulkarnain DEDI CANDRA Diah E. Anggraini Diah Esti Anggraini Dian Neli Pratiwi Dian Neli Pratiwi Dian Neli Pratiwi Dian Neli Pratiwi Dwi Asmi Dwi Ayu Febriyani Edi Santoso Eko A. Srihanto Eko Agus Prihanto Eko Agus Srihanto Eko Agus Srihanto Eko Agus Srihanto, Eko Agus Elfita Nova Yunior Elizabeth D. Krismuniarti Elly L. Rustiati Elly L. Rustiati Elly L. Rustiati Elly Lestari Rusitati Elly Lestari Rustiati Elly Lestari Rustiati Elsa Virnarenata Emantis Rosa Enny Saswiyanti Enny Saswiyanti Enny Saswiyanti Erdi Suroso Erdi Suroso Eti Ernawiati Eti Ernawiati Eva Yunita Fahrul Azzami Faiz Zainul Muttaqin Fathan Naufal Ahsan Favorisen R. Lumbanraja Firwantoni Firwantoni Ganis Mustikawati Gina Dania Pratami, Gina Dania Giyono Giyono Giyono Giyono Heri Satria Hestin Oktiani I Gede Swibawa I Wayan Tony Candra Ibransyah, Rasyid Indah A. Lestari Indah Ayu Lestari Indah Ayu Lestari Intan Diani Fardinatri Irwan Nasution Jani Master Jani Master, Jani John Hendri Junaidi Junaidi Krismuniarti, Elisabeth Devi Kurniawati, Yeyen Lestari, Septi Wahyu Lili Chrisnawati Lisa Alfina Damayanti Liza Angeliya Lousanja D. Sa'uddah Lousanja Dira Sa’uddah Lousanja Dira Sa’uddah Lu'lu' Kholidah Fauziah Lu'lu' Kholidah Fauziah M. Kanedi Mahfud Sidik Mahfud Sidik Mahfud Sidik Martha Lulus Lande Masniar Vina Kesti Melya Riniarti Mustikawati, Ganis Nindy Permatasari Novianasari, Tika Nugraha, M. Aqwam Nuning Nurcahyani Patrice Kevin Marcus Pertiwi, Vindo R. Pratiwi, Dian N. Pratiwi, Dian Neli Putri Ayu Febrina Putri, Chicka R.P. Raden Fadly Bayu Dwiyoga Resti Puspa Kartika Sari Resti Puspa Kartika Sari Rinawati Rinawati Rochmah Agustrina Rochmah Agustrina Rodliyah, Aini Robby Romaya Wulan Suciningtyas Rustiati, Elly L. Salih Alimudin Sandra, Shifa Saswiyanti, Enny Sekar Pratiwi Sevira Nur Azmi Siti Asiyah Srihanto, Eko A. Sukatmoko Sukatmoko Sukatmoko, Sukatmoko Sunandar Susanto, Alvin Wiwiet Susanto, Gregorius Nugroho Thesalonika, Natasya Tristiyanto Tristiyanto Tugiyono Tugiyono Tugiyono Tugiyono Tugiyono Vidya Kurniasih Viki Ramadan Vindo R. Pertiwi Virnarenata, Elsa Warsono Warsono Waryoko Susandi Winarno Winarno Winda Rahmawati Yanti Yulianti Yanti Yulianti Yeyen Kurniawati Yuni Tinasari Zulfi Arsan Zulkarnain, Danisworo