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All Journal Jurnal Ilmu Lingkungan BIOVALENTIA: Biological Research Journal
Eko Agus Srihanto
Lampung Disease Investigation Center
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Medicine & Pharmacology

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PRIMER OPTIMIZATION AS GENETIC MARKER PRIMER BASED ON CYTOCHROME B GENE ON SUMATRAN ELEPHANT (Elephas maximus sumatranus) NON-INVASIVE SAMPLES Eko Agus Srihanto; Elly Lestari Rustiati; Priyambodo Priyambodo; Dian Neli Pratiwi; Alvin Wiwiet Susanto; Diah Esti Anggraini; Enny Saswiyanti
BIOVALENTIA: Biological Research Journal Vol. 8 No. 1 (2022)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (773.02 KB) | DOI: 10.24233/biov.8.1.2022.216

Abstract

Sumatran elephant is declared as critically endangered by IUCN since 2011. In supporting conservation efforts on sumatran elephant in its natural habitat, molecular data collection of their population needs to be done. Genetic source material is mostly obtained in its natural habitat is its dung/feces. Study on cytochrome B gene as genetic marker from sumatran elephant fecal samples can be done to get additional genetic data as a form of conservation effort. Primer optimization as genetic marker based on cytochrome B gene has been carried out as an early study on sumatran elephant genetics.  Primer optimization program based on cytochrome B genetic marker from sumatran elephant fecal samples was under Higher Education Applied Research funded by The Ministry of Research, Technology, and Higher Education. DNA was isolated from fecal samples of captive sumatran elephants in Elephant Training Center, Way Kambas National Park. Primer optimization was done via amplification by increasing certain temperature. Specificity test was done to ensure that the primer only recognized sumatran elephant DNA. The best result was obtained with the annealing temperature of 52oC
Species Confirmation for Robusta Coffee in Sedayu, Semaka, Tanggamus: Social Forestry Data Base Management Elly Lestari Rustiati; Priyambodo Priyambodo; Eko Agus Srihanto; Dian Neli Pratiwi; Alvin Wiwiet Susanto; Muhammad Febriansyah; Andriyani Wijaya Kusuma; Enny Saswiyanti
Jurnal Ilmu Lingkungan Vol 23, No 4 (2025): July 2025
Publisher : School of Postgraduate Studies, Diponegoro Univer

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jil.23.4.1149-1154

Abstract

Lampung, including the Register 31 Pematang Arahan area which is next to the Bukit Barisan Selatan National Park, is known for its high biodiversity, and its coffee production. Gapoktanhut Lestari Sejahtera, consisting of 13 Forest Farmer Groups (KTH), practiced community-based forest coffee under the KPH Kotaagung Utara, Tanggamus, Lampung. To support the development of biodiversity data base for KPH Kotaagung Utara, molecular-based species identification for robusta coffee has been carried out under BLU LPPM Unila grant year 2023 and in collaboration with the Lampung Disease Investigation Center. Robusta coffee leaf samples from six KTH areas (Bumi Mulyo, Sido Makmur 1, Sido Makmur 2, Sido Makmur 3, Mandiri Jaya, and Murah Rejeki) were collected for molecular species confirmation based on the N-methyl transferase genetic marker. Molecular analysis included DNA extraction, amplification, and electrophoresis conducted at the Lampung Disease Investigation Center. Sequencing was based on the Coffea canephora CAF2 genetic marker. Bioinformatics analysis was performed using the Basic Local Alignment Search Tool (BLAST) and Molecular Evolutionary Genetics Analysis (MEGA) software version 6. Identical sequence lengths of samples in 6 KTH are varied in base pair length and nitrogen base sequences, ranging from 163-530 bp, with 5 out of 6 KTH having a genetic distance of 0.000%, while 1 sample had a genetic distance of 0.006%. It indicates that robusta coffee samples from 6 KTH areas are genetically closely related. It is supported by its phylogenetic tree, the coffee species cultivated by Gapoktanhut Lestari Sejahtera is molecularly confirmed to be robusta coffee, Coffea canephora.
Co-Authors Alvin Wiwiet Susanto Alvin Wiwiet Susanto Andriyani Wijaya Kusuma Diah Esti Anggraini Dian Neli Pratiwi Dian Neli Pratiwi Elly Lestari Rustiati Elly Lestari Rustiati Enny Saswiyanti Muhammad Febriansyah Priyambodo Priyambodo Priyambodo Priyambodo