JBIO: jurnal biosains (the journal of biosciences)
Vol 8, No 1 (2022): JBIO: jurnal biosains (the journal of biosciences)

THE IDENTIFICATION OF ANTIBACTERIAL COMPOUNDS IN CLOVE STEM EXTRACT (Syzygium aromaticum) AND ITS EFFECTIVENESS IN INHIBITING THE GROWTH OF Escherichia coli

Yunita Diyah Safitri (Program Studi Analis Kesehatan, STIKES Karya Putra Bangsa Tulungagung)
Ana Amalia (Program Studi Farmasi, STIKES Karya Putra Bangsa Tulungagung)
Fatimah Fatimah (Program Studi Analis Kesehatan, STIKES Karya Putra Bangsa Tulungagung)
Afidatul Muadifah (Program Studi Farmasi, STIKES Karya Putra Bangsa Tulungagung)



Article Info

Publish Date
15 Mar 2022

Abstract

Clove plants usage in daily life is very diverse, such as kitchen spices, mixtures of various drinks, and medicines. Clove is a spice plant that contains clove oil which is known as an antibacterial agent. The clove stem has a fairly high clove oil content, but this is not widely known by the public. Clove oil is known to have the ability as a fungicide, bactericide, insecticide, and nematicide. The purpose of this study is to identify antibacterial compounds and the effectiveness of clove stem extract on the growth of Escherichia coli. We used GCMS (Gas Chromatography Mass Spectrometry) analysis to identification antibacterial compounds. Antibacterial activity test using paper disc method by measuring the diameter of the inhibition zone formed from clove stem extract at doses of 30%, 50%, and 70% against Escherichia coli. The results of the study were analyzed using ANOVA with significance (p<0.05). The identification of the active compound showed 13 kinds of compounds were found with the dominant compound was alpha-pinene. The results of the antibacterial test showed the antibacterial activity of clove stem extract at doses of 30%, 50% and 70% with inhibition zones formed 12 mm, 12.7 mm, and 14.1 mm respectively. 

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Journal Info

Abbrev

biosains

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

Description

Jurnal Biosains (JBIO) features works of exceptional significance, originality, and relevance in all areas of biological science, from molecules to ecosystems, (ie genetic, microbiology, ecology, biosystematic, biostatistic) including works at the interface of other disciplines, such as chemistry, ...