Jurnal Pembelajaran dan Biologi Nukleus
Vol 8, No 1: Jurnal Pembelajaran Dan Biologi Nukleus Maret 2022

New Zealand Rabbit Spermatozoa Concentration after Giving of Beluntas Leaf Tannin Extract (Pluchea indica)

Susetyarini, Eko (Unknown)
Nurrohman, Endrik (Unknown)



Article Info

Publish Date
07 Mar 2022

Abstract

The purpose of this study was to determine the concentration of spermatozoa in New Zealand rabbits after giving of various types of beluntas leaf tannin extract. This type of research is experimental research. The research design is a one-group post-test design. A sample of 16 rabbits. The sampling technique used is purposive random sampling. The research was conducted at the Chemical Engineering Laboratory of the State Polytechnic of Malang, the Laboratory of Kesimma Medica Malang, the Integrated Laboratory, and the Biology Laboratory of the University of Muhammadiyah Malang. The study was carried out from August to September 2019. The study design used a completely randomized design (CRD) with 4 treatments, namely hydrolyzed, condensed, and pure tannin extract of beluntas leaves and the control group was given aquades orally with a dose of 3 ml/kgbb with 4 replications. repetition times. The procedure of this research 1) Preparation of extract of beluntas leaf tannins, 2) Phytochemical test of extract preparations, 3) Treatment of rabbits as experimental animals, 4) Surgery and collection of rabbit spermatozoa, 5) Observation of spermatozoa using a microscope, 6) Calculating the concentration of spermatozoa. The method of data collection was by calculating the concentration of spermatozoa in each treatment using a hemocytometer and a Neubauer counting chamber using a binocular microscope. Data analysis with one-way Anova test and Duncan 5%. The results showed that the dosage of 3ml/kgbb of various types of beluntas leaf tannin extract had an effect on the spermatozoa concentration of New Zealand rabbits (sig<0.05). The findings of this study are that the best treatment that can reduce the number of spermatozoa in New Zealand rabbits is the condensed tannin extract of Beluntas leaves.

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Journal Info

Abbrev

nukleus

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media

Description

Jurnal Pendidikan Biologi merupakan jurnal elektronik yang merupakan wadah penerbitan artikel penelitian original yang terkait dengan penelitian pendidikan biologi. Jurnal ini dikelola oleh Program Studi Pendidikan Biologidibawah naungan LPPM Universitas Labuhanbatu. Jurnal ini diterbitkan dua kali ...