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All Journal Jurnal Pendidikan Indonesia Biosfer: Jurnal Pendidikan Biologi Jurnal Pembelajaran dan Biologi Nukleus BIO-EDU: Jurnal Pendidikan Biologi Bioscientist : Jurnal Ilmiah Biologi Biocaster : Jurnal Kajian Biologi Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Nurrohman, Endrik
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Social Sciences

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Extract of Beluntas (Pluchea indica) as an Antibacterial Towards Streptococcus mutans ATCC 25175 Causes of Dental Carries Nurrohman, Endrik; Pantiwati, Yuni; Susetyarini, Eko; Umami, Erika Khoirul
BIO-EDU: Jurnal Pendidikan Biologi Vol 6 No 1 (2021): BIO-EDU: Jurnal Pendidikan Biologi Volume 6 Nomor 1 Tahun 2021
Publisher : Jurusan Pendidikan Biologi, Fakultas Ilmu Pendidikan, Universitas Timor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32938/jbe.v6i1.992

Abstract

Abstract The purpose of this study is to analyze the antibacterial activity of various concentrations of beluntas leaf extract by examining the inhibition zone’s diameter of Streptococcus mutans ATCC 25175 bacteria. This type of research is experimental. The research was conducted at the Chemical Engineering Laboratory of State Polytechnic of Malang and the Biology Laboratory of the University of Muhammadiyah Malang. The study was conducted from October to December 2020. The research design used a completely randomized design (CRD) with 7 treatments and 3 replications. The standard of turbidity of bacterial isolates used Mcferland 108. Research design was Posttest-Only Control Design. Data analysis used one-way ANOVA and Duncan's continued test of 5%. The results showed that there was an effect of giving various concentrations of beluntas leaf extract on the inhibition zone of Streptococcus mutans bacteria. The results of Duncan's analysis showed that the concentration of 25% had the most significant effect compared to other treatments and was significantly different from other treatments but rather far from the positive control treatment result. The findings of this study is the content of active compounds in beluntas leaves have an effect as antibacterial towards Streptococcus mutans, also, at a concentration of 5% with minimum inhibitory concentration (khm).
Studi In-Vivo Antifertilitas Stigmasterol Daun Beluntas (Pluchea indica L.): Pengamatan Konsentrasi dan Motilitas Spermatozoa Fatah, M. Arya; Susetyarini, Eko; Waluyo, Lud; Nurrohman, Endrik
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.11295

Abstract

Low public participation in contraception is one of the factors contributing high population density in Indonesia. Indonesia is known as a paradise for medicinal plants, there are many herbs that have the potential to be developed as contraceptives, one of which is the beluntas plant. This study aims to determine the effect of administering various doses of beluntas leaf stigmasterol on the concentration and motility of spermatozoa. The research was conducted at the Biomedical Laboratory, Muhammadiyah University of Malang, Campus 2 for 45 days. This type of research uses true experimental research with a RAL experimental design, consisting of four treatments and six replications. The doses for each treatment are P1 (0.25mg/gbb), P2 (0.5mg/gbb), P3 (0.75mg/gbb) and control (water). The results showed that administering the entire dose of stigmasterol had a significant effect on the concentration and motility of spermatozoa (P<0.05). Spermatozoa concentration P1 (11.83 ± 1.835), P2 (8.17 ± 1.472), P3 (6.33 ± 1.033), control (21.83 ± 3.312). Spermatozoa motility P1 (32.23 ± 25.406), P2 (19.85 ± 7.173), P3 (17.16 ± 2.801), control 975.23 ± 14.417). Stigmasterol from beluntas leaves has potential as a natural antifertility agent in men.
New Zealand Rabbit Spermatozoa Concentration after Giving of Beluntas Leaf Tannin Extract (Pluchea indica) Susetyarini, Eko; Nurrohman, Endrik
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 8, No 1: Jurnal Pembelajaran Dan Biologi Nukleus Maret 2022
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v8i1.2473

Abstract

The purpose of this study was to determine the concentration of spermatozoa in New Zealand rabbits after giving of various types of beluntas leaf tannin extract. This type of research is experimental research. The research design is a one-group post-test design. A sample of 16 rabbits. The sampling technique used is purposive random sampling. The research was conducted at the Chemical Engineering Laboratory of the State Polytechnic of Malang, the Laboratory of Kesimma Medica Malang, the Integrated Laboratory, and the Biology Laboratory of the University of Muhammadiyah Malang. The study was carried out from August to September 2019. The study design used a completely randomized design (CRD) with 4 treatments, namely hydrolyzed, condensed, and pure tannin extract of beluntas leaves and the control group was given aquades orally with a dose of 3 ml/kgbb with 4 replications. repetition times. The procedure of this research 1) Preparation of extract of beluntas leaf tannins, 2) Phytochemical test of extract preparations, 3) Treatment of rabbits as experimental animals, 4) Surgery and collection of rabbit spermatozoa, 5) Observation of spermatozoa using a microscope, 6) Calculating the concentration of spermatozoa. The method of data collection was by calculating the concentration of spermatozoa in each treatment using a hemocytometer and a Neubauer counting chamber using a binocular microscope. Data analysis with one-way Anova test and Duncan 5%. The results showed that the dosage of 3ml/kgbb of various types of beluntas leaf tannin extract had an effect on the spermatozoa concentration of New Zealand rabbits (sig<0.05). The findings of this study are that the best treatment that can reduce the number of spermatozoa in New Zealand rabbits is the condensed tannin extract of Beluntas leaves.
Knowledge and Thinking Skills in Li-Pro-GP Model of Instruction Pantiwati, Yuni; Kusniarti, Tuti; Permana, Fendy Hardian; Nurrohman, Endrik; Amin, Astuti Muh.; Sari, Tasya Novian Indah
Jurnal Pendidikan Indonesia Vol 11 No 4 (2022): Desember
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (468.136 KB) | DOI: 10.23887/jpiundiksha.v11i4.48367

Abstract

Surveys from some research on Indonesian student’s literacy skills showed irregularity with Indonesia's literacy rate. Therefore, it can be said that most of Indonesians have already been literate, in terms of education. Nonetheless, their literacy skills in common still remains weak. Moreover, student’s low literacy skills are deemed as the result of low interest at reading. A number of problems in reading skill is closely associated with limited reading experiences and practices. The current research aims to describe student’s knowledge and thinking skills in a Li-Pro-GP model of instruction. The syntax of this learning model is project-based learning that is integrated with the School Literacy Movement (SLM) and Character Education Reinforcement (CER) programs. The research fell into a descriptive research model.  Research subjects, seventh graders attending IPA instructions. Data of the research were analyzed descriptively, which indicated improvement in terms of knowledge and thinking skills amidst students. In addition, the highest level of language use was found at the test for Aspect C2, C4, C5, and C6, whilst the highest level of conceptual truth was indicated at Aspect C3. On the other hand, the lowest level of giving argumentation appeared at all aspects. In sum, the highest level of skills was indicated by the language use, while the lowest at the student’s confidence in giving argumentation.
Application of the Li-Pro-GP learning model to improve students’ conceptual understanding and creativity Yuni Pantiwati; Permana, Fendy Hardian; Kusniarti, Tuti; Sari, Tasya Novian Indah; Nurrohman, Endrik
Biosfer: Jurnal Pendidikan Biologi Vol. 15 No. 1 (2022): Biosfer: Jurnal Pendidikan Biologi
Publisher : Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21009/biosferjpb.25800

Abstract

Concept understanding and product creativity of Ma'arif class VII A junior high school students are less than optimal. This problem is because learning activities do not invite students to increase literacy. Based on this, it is necessary to innovate learning. The purpose of this research is to apply Li-Pro-GP learning (Project-Based Science Literacy in the integrated scientific literacy movement strengthening character education) to improve conceptual understanding and creativity of learning poster products. The type of research used is quasi-experimental research (quasi-experimental) using a one-group pretest-posttest design. The research population is seventh-grade junior high school students. While sampling using purposive sampling. The research sample is all Ma'arif junior high school students in class VII A. Data collection techniques use tests and documentation with research instruments, namely written test questions in the form of essays used to pretest and posttest. The measurement of the creativity of the learning poster product uses the product creativity rubric. Data analysis using paired t-test with the help of data processing applications IBM SPSS 26 and Ms.Excel. The results showed that: 1) there was an effect of using the Li-Pro-GP learning model on the ability to understand concepts; 2) there is an effect of using the Li-Pro-GP learning model on the creativity of the learning poster product.
Blood profile of white rats (Rattus norvegicus): Safety study of stigmasterol compounds from beluntas leaves (Pluchea indica) as antifertility Susetyarini, Eko; Latifa, Roimil; Nurtjahyani, Supiana Dian; Nurrohman, Endrik
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 10 No. 01 (2025): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v10i01.1288

Abstract

This study aimed to examine the blood smear profile of male white rats after treatment with various doses of stigmasterol from Pluchea indica leaves. The experimental study used the post-test control design group. The study was conducted in the several accredited laboratories in Malang and Bandung cities. The study was conducted from June to September 2024. The research sample of stigmasterol from P. indica leaves came from the UPT materia medica of Batu City. The sampling technique was purposive random sampling. Three doses of stigmasterol (0.125 mg/kg bw, 0.25 mg/kg bw, 0.5 mg/kg bw), and control (aquades) with seven replications. Observations of preparations for each treatment group used a binocular microscope. Photos of the observation results were analyzed for cell shape, and the number of visible white blood cells was calculated. Data analysis used one-way ANOVA and Duncan’s further test. The results showed a stigmasterol dose hadtigmasteroland number of erythrocytes in the blood of white rats. The findings of this study were that dose 3 (0.5 mg/kg bw) had the lowest number of leukocytes, and further research is needed as a safe dose of the stigmasterol compound in P. indica leaves, which has the potential as a natural antifertility drug.
Pengaruh Pemberian Stigmasterol Daun Beluntas (Pluchea indica) terhadap Kerusakan Histologi Hati Tikus Putih (Rattus novergicus) Jantan sebagai Sumber Belajar Biologi Zahidah, Ashfa; Susetyarini, Eko; Permana, Tutut Indria; Nurrohman, Endrik; Husamah, Husamah
Biocaster : Jurnal Kajian Biologi Vol. 5 No. 3 (2025): July
Publisher : Lembaga Pendidikan, Penelitian, dan Pengabdian Kamandanu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/biocaster.v5i3.491

Abstract

The implementation of a healthy lifestyle with a “back to nature” approach has become a global trend, marked by the increasing consumption of natural-based medicines which are considered relatively safer compared to synthetic drugs. The beluntas plant (Pluchea indica L.) is known to contain active compounds such as alkaloids, tannins, and flavonoids, including stigmasterol which has the potential as a pharmacological agent. This study aims to determine the effect of administering stigmasterol from beluntas leaves at various doses on liver histological damage. The study was conducted at the Biomedical Laboratory of the Faculty of Medicine, University of Muhammadiyah Malang for 28 days. The type of study used true experiment research with a RAL experimental design, consisting of four groups and six replications. The doses of each treatment were P1 (0.125 mg/kgBW), P2 (0.25 mg/kgBW), P3 (0.5 mg/kgBW), and the control group. The results of the one-way ANOVA test showed that the data on the effect of the administration of stigmasterol from beluntas leaves showed significant differences in liver cell damage in male white rats (Rattus novergicus) in parenchymatous degeneration (p = 0.010), in hydropic degeneration (p = 0.000), in karyorrhexis cells (p = 0.020), in karyolysis cells (p = 0.000), and in inflammatory cells (p = 0.021). However, the results of the one-way ANOVA test showed no significant effect on the administration of stigmasterol to pyknosis cells (p = 0.409 > 0.05). Meanwhile, the administration of stigmasterol in treatment 1 effectively reduced hydropic and prenchymatous degeneration but actually increased damage to karyolysis cells, karyorrhexis cells and inflammatory cells. This study of the effect of stigmasterol from beluntas leaves on liver histology can be used as a source of learning Biology in phase F in SMK Kesehatan class XI Pharmacology, especially in understanding drugs related to disorders of the body's system.
Co-Authors Astuti Muh. Amin Fatah, M. Arya Fendy Hardian Permana Husamah Husamah Lud Waluyo Roimil Latifa Rr. Eko Susetyarini Sari, Tasya Novian Indah Supiana Dian Nurtjahyani Tuti Kusniarti Tutut Indria Permana Umami, Erika Khoirul Yuni Pantiwati Zahidah, Ashfa