Study Some Kinetic Parameters for Purified Arginase from Two Different Sources: Red Blood Cells and Serum of( Healthy and TypeII Diabetic Patient)

Fayhaa M. Khaleel
Essam F.A.Al-Jumaili

Indian Journal of Forensic Medicine & Toxicology
Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology

Kinetics parameters for arginase that purified from RBCs, and serum (control, and diabetic patients)revealed that the Km values are (200, 416, and 333mM), Vmax values are (47, 5.5, and 9 U/min),andThe values of catalytic constant Kcat are (174, 45.8, and 81.8 min-1). The effect of atrovastatin was alsostudied on serum arginase, the arginase activity is decreasing by increasing statin concentration, whereinat the lower concentration (0.112 mg/ml) the allosteric regulation percentage is 26% and it is ongoing untilreaching its maximum (85%) at the highest concentration (0.9mg/ml). In conclusion affinity of arginase tothe L-arginine, and its velocity is more in RBCs, and serum patients compared with serum controls, andstatin appears to be an allosteric regulator for arginase.