Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 36, No 1 (2004)

Differentiation and identification of Aedes albopictus Skuse from several population in Indonesia based on genetic polymorphisms

Budi Mulyaningsih, Budi Mulyaningsih (Unknown)



Article Info

Publish Date
18 Nov 2015

Abstract

Background: Aedes albopictus play important role in the spread dengue virus, it is spread widely in Indonesia. Geographical condition of Indonesia which shows varying climatology, biogeography and environmental factors seem to be very possible to cause the difference in the genetic structure of Ae. albopictus.Objective: The aim of this study is to determine the genetic polymorphism of each Ae. albopictus population.Methods: Genomic DNA was extracted from individual mosquitoes from 4 geographic populations of Ae, albopictus and amplified in PCR reactions using single primers of arbitrary nucleotide sequence.Result: The DNA polymorphism level of Ae. albopictus population originated from Yogyakarta is 80.77%, followed by population originated from Padang is 57.14%, Banjar is 40.74% and Timika is 37.50%. Conclusion: The genetic structure of the Ae. albopictus populations shows obvious differences, and they shows that they are from different group and separated with the various difference percentage.Key words: genetic polymorphism - population - Ae. albopictus - dengue virus - RAPD-PCR.

Copyrights © 2004






Journal Info

Abbrev

bik

Publisher

Subject

Immunology & microbiology Neuroscience

Description

Journal of the Medical Sciences (JMedSci) or Berkala Ilmu Kedokteran (BIK) is an international, open-access, and double-blind peer-reviewed journal, published by Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada Yogyakarta Indonesia. JMedSci aiming to communicate high-quality ...