BiosciED: Journal of Biological Science and Education
Vol. 3 No. 1 (2022): BiosciED June 2022

Escherichia coli Bacteria Test on Polluted Meatballs With Several Variations of Positive Control Concentration: Escherichia coli Bacteria Test on Polluted Meatballs With Several Variations of Positive Control Concentration

Alfi sophian (National Food and Drug Testing Development Center, National Agency of Drug and Food Control of Indonesia (BPOM))



Article Info

Publish Date
01 Jun 2022

Abstract

Escherichia coli bacteria test on contaminated meat processed food products with several variations of positive control concentrations was carried out to provide additional information about determining the LOD value in the test method for the detection of Escherichia coli pathogenic bacteria. The purpose of this study was to detect and identify bacterial contamination of Escherichia coli ATCC 25922 in contaminated meat processed food products with variations in the concentration of positive control. The method used to identify is the enrichment method, using enrichment media to grow the suspected target bacterium Escherichia coli ATCC 25922 spiked in meat-processed food products, followed by isolation using selective media and ending with confirmation and affirmation tests. The samples used were 10 packages of processed meat food products. The samples were then spiked using various concentrations of positive control Escherichia coli 5, 10, 50, and 100 colonies/gr. The data from the research showed that all samples from the treatment of variations in the concentration of spike positive controls were detected and identified the presence of Escherichia coli ATCC 25922. Based on the results of this study, it can be concluded that all samples spiked with various variations in the concentration of positive control were detected and identified Escherichia coli ATCC 25922.

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Journal Info

Abbrev

bed

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Other

Description

BioscieED is a Peer-Reviewed journal that is published 2 (two) times a year in June and December. BiosciED is an acronym for Biological Science and Education which represents the focus and scope of the Biology Education journal. The focus and scope of the journal include research related to the ...