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All Journal Journal of Food and Pharmaceutical Science Wahana-Bio: Jurnal Biologi dan Pembelajarannya BIOEDUSCIENCE JRST (Jurnal Riset Sains dan Teknologi) Indonesian Food Science and TechnologyJournal FoodTech: Jurnal Teknologi Pangan Journal of halal product and research (JHPR) Keluwih: Jurnal Sains dan Teknologi BiosciED: Journal of Biological Science and Education Journal of Experimental and Clinical Pharmacy (JECP) Muhammadiyah Journal of Nutrition and Food Science (MJNF) Food Scientia: Journal of Food Science and Technology Jurnal Farmasi Sains dan Praktis Asia Pacific Journal of Sustainable Agriculture, Food and Energy (APJSAFE) Eruditio : Indonesia Journal of Food and Drug Safety Journal of Food and Pharmaceutical Sciences Borneo Journal of Pharmacy Kupang Journal of Food and Nutrition Research
Sophian, Alfi
Pusat Pengembangan Pengujian Obat Dan Makanan Nasional, Badan Pengawas Obat Dan Makanan. Jl. Percetakan Negara, No. 23. Jakarta Pusat,10560, Indonesia
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PENGUJIAN SALMONELLA TYPHIMURIUM ATCC 14028 PADA PRODUK SOSIS, NUGGET, BAKSO, OTAK-OTAK, TEMPURA DAN CILOK MENGGUNAKAN KIT RAPID TEST Sophian, Alfi; Muindar, Muindar
Journal of Experimental and Clinical Pharmacy (JECP) Vol 1, No 1 (2021): Februari, 2021
Publisher : Poltekkes Kemenkes Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52365/jecp.v1i1.198

Abstract

Pengujian Salmonella typhimurium ATCC 14028 menggunakan rapid test kit dilakukan di laboratorium pengujian mikrobiologi dan biologi molekuler Badan Pengawas Obat dan Makanan di Gorontalo. Penelitian dengan menggunakan rapid test kit merupakan penelitian sederhana yang membutuhkan waktu yang relatif lebih singkat dibandingkan dengan menggunakan teknik konvensional. Tujuan penelitian ini adalah untuk melihat cara kerja rapid test kit dalam mendeteksi bakteri Salmonella typhimurium ATCC 14028 yang dibubuhi beberapa produk olahan pangan. Sampel terdiri dari 12 ulangan dengan 6 jenis produk pangan diantaranya sosis, nugget, bakso, otak-otak, tempura dan cilok. Kontrol positif dibuat dari kultur Salmonella typhimurium ATCC 14028 fase 2, sedangkan kontrol negatif menggunakan sampel sosis, nugget, bakso, otak-otak, tempura dan cilok yang disterilkan. Konsentrasi kontrol positif yang digunakan diambil dari budaya kerja keruh dan disamakan menurut standar Macfarland 1. Analisis data dilakukan secara kualitatif berdasarkan perubahan warna kit pada sampel dibandingkan dengan kontrol positif dan negatif. Berdasarkan hasil penelitian didapatkan bahwa semua sampel terdeteksi Salmonellatyphimurium ATCC 14028. Penelitian ini menyimpulkan bahwa rapid test kit dapat digunakan untuk mendeteksi Salmonella typhimurium ATCC 14028 pada produk sosis, nugget, bakso, otak-otak, tempura dan cilok.Kata kunci: Cepat, Test Kit, Salmonella typhimurium, Otak-Otak.
Analisis Hasil Isolasi DNA dari Bulu Ayam yang Dicuplik dari Pangkal Bulu Muda, Pangkal Bulu Tua dan Ujung Bulu Alfi Sophian
BIOEDUSCIENCE Vol 5 No 2 (2021): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (326.903 KB) | DOI: 10.22236/j.bes/526211

Abstract

Background: The goal was to provide information on the use of DNA templates in chicken samples so that the molecular research sampling process may employ feathers instead of hurting the test animals. Method: The sample used consisted of 10 Bangkok chickens which were sampled for young feathers and old feathers and the tips of the feathers. Quantitative techniques by comparing the results of DNA isolation which were analyzed using a nano photometer and then confirmed using real-time PCR with the SYBR green method. Result: The analysis of purity and concentration showed that at the base of young chicken feathers, the average value of purity was at 1,790, with an average value of the concentration of 4,210. At the base of the old feather, the average value of purity was 0.638, with an average concentration value that was not detected. Likewise, at the tip of the feather, the average purity value is 0.894 and the concentration value is not detected. Confirmation tests performed on all samples using the real-time PCR melt curve method showed that all samples were detected with a Tm value of 78.5 for young feathers, 78.5 for old feathers, 79.0 for positive controls and 78.7 for positive controls, while negative controls were not detected. Conclusion: DNA isolation can be carried out at the base of the young feathers, the base of the old feathers and the tips of the feathers.
Detection of Species DNA in Chicken Meatball Products Using NGF Genes as Molecular Markers: Detection of Species DNA in Chicken Meatball Products Using NGF Genes as Molecular Markers Alfi Sophian
BiosciED: Journal of Biological Science and Education Vol. 2 No. 2 (2021): BiosciED December 2021
Publisher : FKIP, Universitas Palangka Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37304/bed.v2i2.3422

Abstract

Species DNA detection in chicken meatball products using the NGF gene as a molecular marker was carried out to see if the NGF gene could be used to test species DNA detection in chicken meatball processed food products. The test method used in this study is the SYBR green test method which is read using real-time PCR and the extraction technique used is the centrifuge column technique. The test results in this technique can be in the form of Ct (Cycling) and Tm (Melting Temperature) values. The results showed that the DNA isolation carried out showed that the concentration and purity of the isolated DNA were in the range of 51,100 ng/µL – 52,300ng/µL with an average of 51,883 ng/µL. As for the value of purity is the absorption value measured at wavelength A260/A280, the results are obtained in the range between 1,850 – 1,920 with an average of 1,880. The results of the real-time PCR analysis obtained the Ct value of the chest sample at 21.10, the Ct LOD value at 25.20 and the positive control Ct value at 20.30. For the Tm value of the busty sample at 78.20, the Tm LOD value at 78.80 and the positive control Tm value at 79.10. Based on the results of this study, it can be concluded that the genetic markers of the NGF gene can be used to test specific DNA detection of chicken species in processed chicken meatball products so that this test method can be used to detect species DNA.
Escherichia coli Bacteria Test on Polluted Meatballs With Several Variations of Positive Control Concentration: Escherichia coli Bacteria Test on Polluted Meatballs With Several Variations of Positive Control Concentration Alfi sophian
BiosciED: Journal of Biological Science and Education Vol. 3 No. 1 (2022): BiosciED June 2022
Publisher : FKIP, Universitas Palangka Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37304/bed.v3i1.4908

Abstract

Escherichia coli bacteria test on contaminated meat processed food products with several variations of positive control concentrations was carried out to provide additional information about determining the LOD value in the test method for the detection of Escherichia coli pathogenic bacteria. The purpose of this study was to detect and identify bacterial contamination of Escherichia coli ATCC 25922 in contaminated meat processed food products with variations in the concentration of positive control. The method used to identify is the enrichment method, using enrichment media to grow the suspected target bacterium Escherichia coli ATCC 25922 spiked in meat-processed food products, followed by isolation using selective media and ending with confirmation and affirmation tests. The samples used were 10 packages of processed meat food products. The samples were then spiked using various concentrations of positive control Escherichia coli 5, 10, 50, and 100 colonies/gr. The data from the research showed that all samples from the treatment of variations in the concentration of spike positive controls were detected and identified the presence of Escherichia coli ATCC 25922. Based on the results of this study, it can be concluded that all samples spiked with various variations in the concentration of positive control were detected and identified Escherichia coli ATCC 25922.
DETECTION OF SALMONELLA TYPHIMURIUM BACTERIA ON BAKERY PRODUCTS SAMPLES USING BOILING ISOLATION TECHNIQUE Alfi Sophian; Ratna Purwaningsih; Muhammad Tri Sutrisno; Purwadi Purwadi; Arif Wahyudi
Jurnal Farmasi Sains dan Praktis Vol 8 No 3 (September-December 2022)
Publisher : Universitas Muhammadiyah Magelang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31603/pharmacy.v8i3.5550

Abstract

Detection of Salmonella typhimurium ATCC 14028 bacteria in bakery product samples by real-time PCR using boiling isolation technique. The basis of this research is to have an impact on economic value in carrying out the confirmation test for Salmonella typhimurium ATCC 14028, where testing is carried out conventionally will require large costs, so it is necessary to innovate in terms of modifying the testing phase so that it is more effective and efficient. The purpose of this study was to see whether the boiling isolation technique could be used for the detection test for Salmonella typhimurium ATCC 14028 on bacterial product samples. The sample in this study consisted of 15 types of bacterial product samples spiked with Salmonella typhimurium ATCC 14028 cultures that had been cultured into phase 2 working cultures. The method used in this study was qPCR analysis using the SYBR Green method. The results of real-time PCR analysis obtained Ct values in the range 7.55 - 8.91 with an average of 8.28 and a Tm value in the range 85.50 - 86.20 with an average of 85.77 Based on these data it can be concluded that the detection of Salmonella typhimurium bacteria ATCC 14028 with real-time PCR using boiling isolation technique can be applied for testing on bakery product samples.
Analisis DNA Hasil Isolasi Pada Produk Pangan Olahan Ikan (Surimi Ikan) Menggunakan Nano Photometer Sofia Dyah Utami; Sri Utaminingsih; Alfi Sophian
JRST (Jurnal Riset Sains dan Teknologi) Volume 7 No. 1 Maret 2023: JRST
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (725.027 KB) | DOI: 10.30595/jrst.v7i1.15180

Abstract

Analisis mutu DNA untuk melihat kualitas DNA hasil isolasi merupakan sebuah teknik analisis yang dapat digunakan untuk mendukung pengujian bidang biologi molekuler yang menggunakan instrumen PCR atau real-time PCR. Tujuan dari penelitian ini adalah untuk menganalisis kualitas DNA hasil isolasi pada sampel produk pangan olahan ikan.  Manfaat penelitian ini diharapkan dapat menjadi sumber informasi yang mendukung penelitian di bidang molekuler pada ruang lingkup pengawasan mutu dan autentifikasi pada produk pangan olahan ikan. Analisis DNA hasil isolasi dianalisis menggunakan NanoPhotometer untuk mengukur nilai konsentrasi dan kemurnian dengan panjang gelombang A260/A280. Data yang diperoleh dari hasil pengukuran kemudian diinterpretasikan menggunakan nilai uji rata-rata untuk menganalisis mutu DNA hasil isolasi. Data hasil penelitian menunjukan nilai konsentrasi DNA berada pada kisaran 14,5 – 17,8 dengan rata-rata nilai konsentrasi berada pada 16,1. Untuk nilai kemurnian DNA hasil isolasi berada pada kisaran 1,89 – 2.07 dengan rata-rata nilai kemurnian berada pada 1,97.  Dengan hasil tersebut maka dapat disimpulkan bahwa hasil isolasi DNA yang diukur menggunakan nano fotometer menunjukkan nilai kualitas DNA hasil isolasi yang baik.
Analysis of Purity and Concentration of DNA Isolation Results on Chondroitin Samples Sri Utaminingsih; Alfi Sophian
BiosciED: Journal of Biological Science and Education Vol. 3 No. 2 (2022): BiosciED December 2022
Publisher : FKIP, Universitas Palangka Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37304/bed.v3i2.5425

Abstract

Analysis of purity and concentration of isolated DNA is a way to see the quality of isolated DNA. This research was conducted as an initial stage to give. Information about research conducted in future research. The purpose of this research is to provide references and information regarding DNA isolation techniques in chondroitin samples, so that they can be used in similar studies. The sample consisted of a sample of chondroitin which was weighed 50 mg and tested 10 times. The isolation method used in this study is the centrifuge column isolation method, while the purity and concentration analysis were analyzed using a nano photometer which was read at a wavelength of A260/A280. The value shown from the nano photometer was then analyzed statistically using the average test to determine the interpretation of the results from the data obtained. The results of DNA isolation obtained that the DNA concentration values ​​tested were in the range of 39.10 - 54.70 with an average concentration value of 45.15. The value of the purity of the isolated DNA tested was in the range of 2.16 – 2.28 with an average purity value of 2.22. Based on the results of the DNA confirmation test of the isolation carried out using real-time PCR, it showed that the isolated sample was amplified at a value of Ct 36.43 while the positive control was amplified at Ct 32.49. Based on the research results, it was found that all samples tested showed good average values ​​of DNA concentration and purity so that the results of the DNA isolation tested could be used as templates in real-time PCR analysis.
Analysis of Purity and Concentration of DNA Isolated in Dragonfly (Onychogomphus forcipatus) Alfi Sophian; La Ode Nasir
Wahana-Bio: Jurnal Biologi dan Pembelajarannya Vol 14, No 2 (2022): Wahana-Bio Edisi November 2022
Publisher : Program of Biology Education, Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/wb.v14i2.13209

Abstract

Analysis of the purity and concentration of DNA isolation results was carried out using samples of dragonflies (Onychogomphus forcipatus) species of insects. This research is important because there are many kinds of research in the molecular field that require good DNA isolation techniques to support the success of the research. The purpose of this paper is to provide information about the quality of the isolated DNA seen using the purity and concentration parameters. The extraction method uses the centrifuge column method. The isolated data were analyzed statistically using the average test. The results of DNA isolation showed that the average concentration value was in the range of 60.10 – 69.95. The analysis of the purity of the isolated DNA read at the A260/A280 ratio was in the range of 1.817 – 1.929, while the purity analysis at the A260/A230 ratio was in the range of 0.760 – 0.822. Based on the results of the study, it can be concluded that the analysis of the purity and concentration of DNA from the isolation of dragonfly samples was in a good category at the A260/A280 ratio, while the purity read at the A260/A230 ratio was below the value categorized as good.
Analisis kemurnian dan konsentrasi DNA hasil isolasi pada pembuatan DNA baku spesies tikus: Analisis kemurnian dan konsentrasi DNA hasil isolasi pada pembuatan DNA baku spesies tikus Alfi Sophian; Andi Syukur
Eruditio : Indonesia Journal of Food and Drug Safety Vol 1 No 2 (2021): Edisi Juni
Publisher : Badan Pengawas Obat dan Makanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (568.449 KB) | DOI: 10.54384/eruditio.v1i2.75

Abstract

Analysis of the purity and concentration of isolated DNA in the manufacture of standard rat DNA was carried out to see whether the isolation carried out could produce good quality DNA. The purpose of this study is to provide information on the manufacture of raw DNA in species DNA testing where the raw material that has been purchased so far made from synthetic materials can be more economical if using DNA material derived from the meat raw material of the target species. The DNA extraction method used is the column spin method or column centrifuge using the Intron Patho Gene-Spin (Viral DNA/RNA) extraction kit. Analysis method of concentration and purity of isolated DNA was analyzed based on the average value of concentration and purity which was read using a nanophotometer. Based on the results of the research conducted, the results of the isolated DNA concentration values ​​were in the concentration range of 41,250 ng/ mL to 42,300 ng/mL, with the average concentration of isolated DNA was 41,777 ng/mL. For the value of the purity of the isolated DNA whose absorbance was read using a nanophotometer at a wavelength of A260/A280, the results were between 2,301 to 2,384 with the average value of purity being at 2,326. This study concludes that all the extracted samples that showed the results of the DNA analysis produced were included in the good DNA category.
Aplikasi Teknik Double Wash unutk Isolasi DNA Spesies pada Sampel Cangkang Kapsul Lunak: Application of Double Wash Technique for Species DNA Isolation in Soft Capsule Shell Samples Marcella Sutanta; Diana Tri Wulan; Yusrina Nabila; Alfi Sophian
Eruditio : Indonesia Journal of Food and Drug Safety Vol 2 No 1 (2021): Edisi Desember
Publisher : Badan Pengawas Obat dan Makanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (509.378 KB) | DOI: 10.54384/eruditio.v2i1.78

Abstract

The presence of gelatin components can be identified through fat, protein and DNA. DNA can be obtained through the DNA isolation process which is the process of separating DNA from other components of the cell. The DNA purification stage is the most important thing in DNA isolation because at this stage it can reduce to a minimum the number of contaminants in DNA isolates to determine the purity and concentration of the DNA isolate obtained. The purpose of this technique is to produce DNA isolates that are low inhibitors or are in the purity range of 1.7-2.1. The extraction method used in this study used the double wash technique, the technique was modified from the test stage following the manual kit used, it's just that the technique used was modified at the washing stage with each washing done twice in the washing section. first and second washing so that a total of four items of washing were carried out. The results of the DNA isolates were then analyzed for the purity and concentration of DNA obtained using a nanophotometer, the DNA isolates obtained were in the range of 4,400 - 5,500 ng/µL with an average of 4,950 ng/µL. As for the purity value measured at the wavelength A260/A280, the results were obtained with a purity range between 1,760 - 1,840 with an average of 1,800. This research concludes that all the extracted samples show the results of DNA isolates that fall into the category of good DNA and meet the requirements needed in molecular analysis.
Co-Authors Abdul Rohman Aditya Anugerah Marusaha Sitorus Andi Syukur Arif Wahyudi Dewi Rahmawati Diana Tri Wulan Eka Putri Juniarti Igirisa Eni Cahyaningsih La Ode Nasir Marcella Sutanta Muhammad Luthfi Amirullah Muhammad Tri Sutrisno Muindar Muindar Muindar, Muindar Nawwaruddin, Hazza Hammam Purwadi Purwadi Ratna Purwaningsih Ratna Purwaningsih Ratna Purwaningsih Rumiyati, Rumiyati Sari, Miftiana Nugraha Sofia Dyah Utami Sri Utaminingsih Sri Utaminingsih Sri Utaminingsih Sri Utaminingsih Tirta Setya Bhakti Utami, Sofia Dyah Yenita . Yusrina Nabila Yustina Yustina