<![CDATA[Introduction: Neem leaves (Azadirachta indica) has many benefits including antibacterial, antifungal and antimalarial. Toxicity tests need to be carried out to determine the effects and safety of using neem during growth and development. Exposure neem 60 mg / L in the early stages of Cyprinus carpio L life induces focal separation and gill lamellae edema. The aim of this study is to determine the effect of neem leaves subchronic exposure to gill lamellar hyperplasia and swimming speed of D.rerio juvenilee.Method: Juvenilee zebrafish of 1.5 months old were exposed to Neem leaf decoction (DDN). To observe gill lamella hyperplasia, D.rerio juvenilee was exposed for 15 days to DDN dose of 50 mg / L, 132 mg / L, and 410 mg / L. On the 16th day, gill tissue was taken and stained them using Hematoxylin Eosin (HE). Gill lamella cell was counted using binocular microscope with 1000x magnification. To observe swimming speed changes, D.rerio juvenilee was exposed to DDN for 30 minutes and analyzed using tracker software. Data were analyzed using One-way analysis of variance (ANOVA) continued with Least Significant Difference (p <0.05).Results: Subchronic exposure of D.rerio juvenilee to DDN doses 132 mg / L and 410 mg / L increase gill secondary lamella hyperplasia significantly compared to controls (p <0.05) but insignificant in dose 50 mg / L. All three doses do not induce swimming speed change compared to the control group (p> 0.05).Conclusion: Subchronic exposing of D.rerio juvenilee to DDN doses of 132 mg / L and 410 mg / L did not induce changes in the swimming speed of D.rerio juvenilee. The three doses were able to increase hyperplasia of gill lamellae.]]>
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