KOVALEN: Jurnal Riset Kimia
Vol. 8 No. 2 (2022): August Edition

Identifikasi Senyawa Fraksi Larut n-Heksana Rimpang Temu Mangga (Curcuma mangga Val) Menggunakan LC-MS/MS dan Review Potensinya sebagai Antibakteri: Identification of Compounds Soluble n-Hexane Fraction of Rhizome of Temu Mangga (Curcuma mango Val) Using LC-MS/MS and Review of its Potential as Antibacterial

Purwantinigsih Sugita (Chemistry Department, Institut Pertanian Bogor)
Risda Fardilya Nihayah (Program Studi Sarjana Kimia, Departemen Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, IPB University,)
Moch Ikkbal Aditya Kasmaran (Program Studi Sarjana Kimia, Departemen Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, IPB University)
Gustini Syahbirin (Departemen Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, IPB University)
Auliya Ilmiawati (Departemen Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, IPB University)
Budi Arifin (Departemen Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, IPB University)
Luthfan Irfana (Departemen Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, IPB University)



Article Info

Publish Date
30 Aug 2022

Abstract

Plants are like the pharmaceutical industry that provides products that have the potential as active medicinal ingredients. In order to search for compounds useful for health, the purpose of this study was to identify secondary metabolites of Curcuma mango (temu mango) growing in the experimental garden of Biopharmaca LPPM IPB and review its activity as antibacterial. Identification begins with phytochemical screening, then separation and purification by chromatographic techniques. Intersection mango simplicia was extracted with methanol, and the crude extract of methanol was partitioned with n-hexane, ethyl acetate, and methanol, respectively. The n-hexane soluble fraction was carried out by phytochemical tests, fractionation by vacuum liquid chromatography (KCV) and radial (KR), and the selected fractions was analyzed by LC-MS/MS. The results of the phytochemical test of the soluble n-hexane fraction showed a positive presence of alkaloids and terpenoids through successive tests with Mayer, Wagner, Dragendorff (alkaloid), and Lieberman-Burchard reagents. Fractionation of the n-hexane soluble fraction with KCV resulted in 9 fractions (H1-H9) and the H5 fraction showed good separation between spots in the n-hexane: ethyl acetate eluent mixture (97:3). Purification of H5 with KR obtained 7 fractions (H5.1-H5.7). Based on LC-MS/MS, the H5.2 fraction was dominated by the terpenoid group, and the results of the literature review showed the contribution of terpenoids as antibacterial compounds

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Journal Info

Abbrev

kovalen

Publisher

Subject

Chemical Engineering, Chemistry & Bioengineering Chemistry

Description

KOVALEN: Jurnal Riset Kimia is a peer-reviewed journal of chemistry published by the Tadulako University, Indonesia. This journal concern with publishing the original research articles, review articles, and the current issues related to chemistry. Publishing frequency 3 issues per year, in April, ...