Effect of Stromal Vascular Fraction and Platelet-Rich Plasma Combination on Polymorphonuclear Cells in Wistar Rats Anal Trauma Model

Erwin Chandra (a:1:{s:5:"en_US"s:24:"RS. Wahidin Sudirohusodo"})
Sulmiati Sulmiati (Division of Pediatric Surgery, Department of Surgery, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia)
Nita Mariana (Division of Pediatric Surgery, Department of Surgery, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia)
Fonny Josh (Division of Plastic and Reconstructive Surgery, Department of Surgery, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia)
Sachraswaty Rachman (Division of Plastic and Reconstructive Surgery, Department of Surgery, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia)
Firdaus Hamid (Department of Microbiology, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia)

Nusantara Medical Science Journal
Volume 7 Issue 2, July - December 2022

Introduction: By relying on polymorphonuclear (PMN) cells, stromal vascular fraction (SVF) and platelet-rich plasma (PRP) contribute significantly in the healing process. Through anal trauma model, this study examined the effect of SVF and PRP combination on PMN counts in vivo. Method: Three groups of twenty-eight eligible Wistar rats anal trauma model randomly assigned to receive SVF and PRP combination (Group A), normal saline (Group B), or healthy controls (Group C). On days 1, 7, and 14, we examined at PMN cell counts in Groups A and B. Group C were sacrificed early to acquire baseline data on PMN cells. Results:  On the first day, Group A had more PMN cells than Group B, but this difference faded by days 7 and 14. On day 1, the test revealed a significant increase in PMN cells (p=0.002). Conclusion:  Combining SVF and PRP can improve PMN cells' acute phase of anal damage repair in vivo