Steroids are secondary metabolites, steroid compounds can be used as antioxidant and anti-cancer compounds.This study aims to determine the levels of steroids in fruit, flour and chayote biscuits using the maceration extraction method, the determination of levels using a UV-Vis spectrophotometer. The results showed that the steroid levels in each sample were 0.235±0.03 gram/100 g, on chayote flour by 0.435±0.02 gram/100 g, and on chayote biscuits by 0.556±0.04 gram/100 g.
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