QISTINA: Jurnal Multidisiplin Indonesia
Vol 1, No 2 (2022): December 2022

Effect of Semen Leaching and Soybean Lecithin Levels in Tris Extenders on the Quality of Preserved Sheep Spermatozoa at 5oC Temperature

Salmin Salmin (Universitas Tadulako)
Marsudi Marsudi (Universitas Sulawesi Barat)
Deka Uli Fahrodi (Universitas Sulawesi Barat)
Hendro Sukoco (Universitas Sulawesi Barat)



Article Info

Publish Date
25 Dec 2022

Abstract

This study aims to study the leaching of semen and the level of soy bean lecithin (Soybean lecithin) in relation to the quality of sheep spermatozoa preserved at 5OC. The study used a Complete Randomized Design (RAL) factorial pattern of 2 x 5 x 3. As the first factor is cement washing (P), consisting of P0 = Fresh cement without washing and P1 = Fresh cement undergoes a washing process. The second factor is the level of soy bean lecithin (L), consisting of L0, L1, L2, L3, and L4. Independent variables are cement washing (P) and soybean lasitin levels (L). As a dependent variable is the quality of spermatozoa at a storage temperature of 5⁰C, which consists of progressive motility, viability, abnormality and intergrity of the spermatozoa membrane. The results of the study obtained that the treatment of cement leaching and soy bean lecithin levels together did not show any noticeable interaction, however, single-seeded lecithin levels showed markedly different influences (P≤0.05) and the washing treatment showed no noticeably different influence on all quality parameters of sheep spermatozoa during five days of storage at 5⁰C. The use of soy bean lecithin as a component of sheep cement extenders is effective against samples of unwashed cement or washed cement. The best quality of sheep spermatozoa was obtained at the treatment of 3% soy bean lecithin levels in Tris extenders against washed and unwashed semen preserved for five days of storage at 5oC with an average progressive motility percentage of 63.18%; viability 72.20%; abnormality 12.43%; and membrane integrity of 72.92%.

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Journal Info

Abbrev

qistina

Publisher

Subject

Religion Economics, Econometrics & Finance Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Nursing

Description

QISTINA: Jurnal Multidisiplin Indonesia is a journal that publishes Focus & Scope research articles, which include: 1. Humanities and social sciences 2. Contemporary political science 3. Education science 4. Religion and philosophy 5. engineering science 6. Business and economy 7. cooperative 8. ...