Jurnal Penelitian Pendidikan IPA (JPPIPA)
Vol 9 No 3 (2023): March

The Characteristics of Diatom in Different Preservation Methods: A Comparation Study

Muhammad Hadi El Amin (Universitas Indonesia)
Nining Betawati Prihantini (Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Kampus UI, Depok, 16424, Indonesia.)



Article Info

Publish Date
31 Mar 2023

Abstract

Diatoms are cosmopolite microalgae with unique characteristics caused of their cells are covered by silica which is called a frustule. Diatom identification is carried out using morphological features that are contained in the frustules. There are some methods for diatom preservation including using Lugol’s iodine, ethanol and formaldehyde solution. This study aims to compare those solutions. Those solutions had prepared with one control and four observations. Lugol’s iodine was prepared with a dose of 1%. Ethanol was used at doses of 20% while the formaldehyde solution was used at 4%. The observed diatom characteristics including the presence of cytoplasm and ornamentation of the diatom frustules (including striae and raphes) were observed to find the distinction in those methods. the presence of cytoplasm in each cell was not too different in the treatment, with the percentage of cytoplasmic cells being 73% (ethanol and formalin) while in Lugol the percentage of cytoplasmic cells was 65% and in the control was 76%.  Based on the results, it was found that there was no significant effect of differences in preservation methods on cells with the cytoplasm or not with a p-value> 0.05.

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Journal Info

Abbrev

jppipa

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Materials Science & Nanotechnology Physics

Description

Science Educational Research Journal is international open access, published by Science Master Program of Science Education Graduate Program University of Mataram, contains scientific articles both in the form of research results and literature review that includes science, technology and teaching ...