INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY
Vol 5 No 1 (2023): Step up to strengthen the laboratory system and prepare for patients care

Analysis of Exopolysaccharides in Lactobacillus casei group Probiotics from Human Breast Milk

Nur Kusmiyati (Agricultural Product Technology Department, Faculty of Agricultural Technology, Universitas Brawijaya, Malang, East Java, Indonesia)
Yuni Puspitasari (Biology Study Program, Faculty of Science and Technology, Universitas Islam Negeri Maulana Malik Ibrahim, Malang, East Java, Indonesia)
Ulfah Utami (Biology Study Program, Faculty of Science and Technology, Universitas Islam Negeri Maulana Malik Ibrahim, Malang, East Java, Indonesia)
Anggeria Oktavisa Denta (Nursing Study Program, Politeknik Negeri Madura, Sampang, East Java, Indonesia)



Article Info

Publish Date
28 Apr 2023

Abstract

Exopolysaccharides get a lot of attention because they can improve the host immune system. Exopolysaccharide is a polysaccharide that is produced and secreted from microbes outside the cell, usually found on the outside of the bacterial structure. The Lactobacillus casei group from human breast milk is thought to have the ability to produce exopolysaccharides. The purpose of the study was to examine the exopolysaccharide of the L. casei group that was isolated from breast milk. The methods used include the gravimetric, the phenol-sulfuric acid and the Fourier Transform Infra-Red (FTIR). The results showed that the L. casei group could produce exopolysaccharides, and had high exopolysaccharide total sugar content. Lactobacillus paracasei had the highest exopolysaccharide and total sugar content of 3660 mg/L and 80.6%, respectively. The FTIR results of the L. casei group exopolysaccharides showed the presence of hydroxyl functional groups O-H (3425.76-3295.98 cm-1), methyl C-H (2930.86-2856.70 cm-1), carbonyl C=O (1660.11-1647.27 cm-1), C-H (1456.16-1373.44 cm-1) and C-O-C ether (1071.08-1056.82 cm-1) which are specific characters of exopolysaccharides. Since the FTIR profile demonstrates that the L. casei group can produce exopolysaccharides, it has greater potential as a a probiotic.

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Journal Info

Abbrev

IJMLST

Publisher

Subject

Health Professions Social Sciences

Description

Contributions will be considered for publication in Indonesian Journal of Medical Laboratory Science and Tehnology (IJMLST) concern kind from research, involvement and theory to functioning matters, education and training. The very wide spectrum of its topics includes: dosimeter, instrument ...