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All Journal AL KAUNIYAH El-Hayah : Jurnal Biologi SAINSTIS Padjadjaran Journal of Dentistry Jurnal Biodjati Jurnal Pengabdian Pada Masyarakat Padjadjaran Journal of Dental Researchers and Students INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Jurnal Riset Biologi dan Aplikasinya Jurnal Abdimas Bina Bangsa Jurnal Kesehatan Gigi dan Mulut (JKGM) Jurnal Terapi Gigi dan Mulut Media Penelitian dan Pengembangan Kesehatan
Ulfah Utami
Universitas Islam Negeri Maulana Malik Ibrahim Malang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Public Health Social Sciences Other

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KARAKTERISASI ENZIM AMILASE DARI BAKTERI BACILLUS MEGATERIUM PADA VARIASI SUHU, PH DAN KONSENTRASI SUBSTRAT Istia'nah, Dina; Utami, Ulfah; Barizi, Ahmad
Jurnal Riset Biologi dan Aplikasinya Vol 2, No 1 (2020)
Publisher : Universitas Negeri Surabaya

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Abstract

Bacillus megaterium merupakan salah satu mikroorganisme potensial yang dapat menghasilkan enzim amilase. Karakterisasi enzim amilase dapat membantu mengetahui kondisi optimum enzim saat bekerja. Penelitian ini bertujuan untuk mengetahui karakteristik enzim amilase yang dihasilkan oleh Bacillus megaterium pada variasi suhu, pH dan konsentrasi substrat. Karakterisasi enzim ditentukan dengan menguji aktivitas enzim pada 3 variasi yakni variasi suhu dengan menggunakan pH netral, variasi pH yang dilakukan pada kondisi suhu optimum hasil perlakuan sebelumnya dan variasi konsentrasi substrat YPSs yang dilakukan pada kondisi suhu dan pH optimum hasil perlakuan sebelumnya. Data aktivitas enzim amilase dianalisis secara deskriptif dengan menentukan nilai aktivitas enzim amilase tertinggi pada setiap perlakuan. Hasil penelitian ini menunjukkan bahwa Bacillus megaterium mampu menghasilkan enzim amilase. Indeks Aktivitas Amilase (IAA) Bacillus megaterium sebesar 2,35 mm. Secara kuantitatif aktivitas enzim amilase ditentukan dengan metode DNS yang untuk mengukur kadar gula reduksi yang diproduksi oleh mikrob dengan tingkat ketelitian yang tinggi. Ekstrak kasar enzim amilase yang diproduksi oleh Bacillus megaterium memiliki karakteristik pada suhu 37oC dengan aktivitas enzim sebesar 1,279 U/mL, sedangkan karakteristik pH berada pada pH 5,0 dengan aktivitas enzim sebesar 1,241 U/mL dan karakteristik konsentrasi substrat berada pada konsentrasi 1,50% dengan aktivitas enzim sebesar 0,548 U/mL.
UJI ANTAGONISME BEBERAPA FUNGI ENDOFIT PADA TANAMAN KENTANG TERHADAP FUSARIUM OXYSPORUM SECARA IN VITRO Izzatinnisa', Izzatinnisa'; Utami, Ulfah; Mujahidin, Ahmad
Jurnal Riset Biologi dan Aplikasinya Vol 2, No 1 (2020)
Publisher : Universitas Negeri Surabaya

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Abstract

Pemanfaatan fungi endofit merupakan salah satu alternatif untuk mengendalikan Fusarium oxysporum pada tanaman kentang (Solanum tuberosum L.) tanpa menimbulkan dampak negatif terhadap lingkungan. Penelitian ini bertujuan untuk mengetahui daya antagonisme fungi endofit hasil isolasi dari tanaman kentang terhadap F. oxysporum secara in vitro. Penelitian ini bersifat deskriptif eksploratif dan eksperimen. Fungi endofit dan F. oxysporum diisolasi dengan metode direct platting, selanjutnya dilakukan pemurnian dan identifikasi fungi. Fungi endofit yang terpilih, dilakukan uji antagonisme terhadap F. oxysporum secara in vitro dengan metode dual culture. Persentase hambatan dianalisis menggunakan uji ANOVA dan uji lanjut Tukey. Hasil penelitian menunjukkan empat isolat fungi endofit berhasil diisolasi dari jaringan daun tanaman kentang yaitu Mucor sp.1, Mucor sp.2, Neoscytalidium sp., dan Aspergillus sp. Fungi patogen hasil isolasi yaitu F. oxysporum. Uji antagonisme dengan metode dual culture menunjukkan semua fungi endofit mampu menghambat pertumbuhan F. oxysporum pada tanaman kentang dengan persentase hambatan yang bervariasi, yaitu Neoscytalidium sp. (73,09%), Mucor sp.2 (70,88%), Mucor sp.1 (59,84%) dan Aspergillus sp. (66,06%). Hasil analisis ANOVA menunjukkan bahwa terdapat pengaruh dari perlakuan yang diberikan (p=0,001). Dengan demikian, fungi endofit hasil isolasi memiliki potensi dalam menghambat fungi patogen tanaman kentang.
Potency of Endophyte Microbes as an Antagonistic Agent for Colletotrichum capsici Causes Anthracnose Disease in Cayenne pepper (Capsicum frustescens) Afifah, Zahroul; Utami, Ulfah
El-Hayah : Jurnal Biologi Vol 6, No 3 (2017): EL-HAYAH (VOL 6, NO 3,September 2017)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v6i3.5804

Abstract

Background: An anthracnose disease caused by pathogenic fungal Colletotrichum capsici has been attacking the cayenne plants either harvested or has not been harvested. This disease must be handled appropriately and quickly because it can reduce the production of chili up to 90%. Recently, anthracnose disease prevention still use chemical fungicide that if applied for long time will cause new impact for environment. Objective: Trichoderma and Bacillus cereus endophytes may be used as antagonistic agents for C. capsici pathogens because they have various antibiotic compounds. Methods: This research uses experimental method. The stages of this study include sterilization of tools and materials, preparation of culture media of fungal and bacteria, rejuvenation of endophytic microbe culture Trichoderma sp. and Bacillus cereus, rejuvenation of  C. capsici pathogen, antagonistic test in vitro using dual culture method. Result and conclusion: The results of in vitro antagonistic tests showed that inhibition percentage of Trichoderma treatment (96%) and combination treatment Trichoderma and B. cereus (97%) is not significantly different. While in B. cereus treatment (11, 88%) significantly different with all of treatments. Endophytes are shown by its dominating growth in petri dishes than C. capsici pathogen or B. cereus endophytes. Furthermore,for endophytes Trichoderma sp continued on in vivo test because it was most effective.The result for incubation period is 3 days after inoculation compared with negative control 2 days. For disease incidence 100%, and for disease intensity that is 61,25% compared with negative control equal to 88,75%.
The Effect of Addition Mn2+ Metal Ions and Incubation Time to Bacillus cereus Cellulase Enzyme Activity from Endophytic Bacteria of Curcuma Rhizome (Curcuma zanthorrizha Roxb.) Sulistyantini, Cahyaning; Utami, Ulfah
El-Hayah : Jurnal Biologi Vol 8, No 2 (2021): EL-HAYAH (VOL 8, NO 2 MARET 2021)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v8i2.12463

Abstract

Cellulase is one of the most widely used enzymes in the industrial world and wastes decomposition process. Bacillus cereus is one of the bacteria that can produce cellulase enzymes that can hydrolyze cellulose to glucose. The addition of cofactors and incubation time can help determine the optimum conditions needed by cellulase enzymes to work optimally. This study aims to determine the effect of adding Mn2+ metal ions and incubation time to the activity of cellulase enzymes from Bacillus cereus endophytic bacteria. This research is experimentally used a Completely Randomized Design (CRD) factorial design with two factor treatments and 3 times repetitions. The first factor is variation of addition Mn2+ metal ions which are 5 mM, 10 mM, and 15 mM, the second is variation of incubation time which are 1 hour, 2 hours, 3 hours, 4 hours, and 5 hours. The data were analyzed using Analysis Of Variance (ANOVA) and if the data significantly affected the parameter, then it would be continued by Duncan Multiple Range Test (DMRT) with the fault level of 5%. The result showed that the interaction of addition Mn2+ metal ions and incubation time affected the cellulase enzyme activity of Bacillus cereus from endophytic bacteria. The highest cellulase enzyme activity obtained from interaction addition Mn2+ metal 10 mM and incubation time 3 hours with an activity 0,335 U/mL, while the lowest cellulase enzyme activity obtained from interaction addition Mn2+ metal 15 mM and incubation time 5 hours with an activity of 0,073 U/mL
PENGUJIAN POTENSI BAKTERI ENDOFIT TERHADAP PERTUMBUHAN POPULASI NEMATODA SISTA KUNING (Globodera rostochiensis) PADA TANAMAN KENTANG (Solanum tuberosum L.) Ulfah Utami; Lilik Hariani; Retno Setyaningrum
SAINSTIS SAINSTIS (Vol 1, No 2
Publisher : Lembaga Penelitian dan Pengembangan Universitas Islam Negeri Maulana Malik Ibrahim Malang.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (916.08 KB) | DOI: 10.18860/sains.v0i0.2301

Abstract

Yellow Nematoda sista (Globodera rostochiensis) is one of interfering plant organism in potatoes plants that bothers farmers in Indonesia. Many controls done by farmers during this time uses chemist pesticide. The using of chemist pesticide  continuously  can  threaten  human  environment  and  healthy.  The existence of endofit bacteria might became one of nematode controlling agent which is hospitable, because endofit bacteria can produce toxic compound in nematode. The aim of this research is to find out the filtrate potential of endofit bacteria to the growth of yellow nematode sista population (Globodera rostochiensis) in potatoes plants.  This research is done in Microbiological and Greenhouse Laboratory, Biology Department, Science and Technology Faculty, UIN Maulana Malik Ibrahim Malang and Pest and Plant Disease Laboratory, Agriculture Faculty, Universitas Brawijaya Malang.  This research is done in December  2011  until  April  2011  by  using  Rancangan  Acak  Lengkap  (RAL) method. Bacterial filtrate is made in a way of making endofit bacteria grow in TSB  media  for  48  hours,  then  disentrifugasi  with  speed  13.000  rpm  for  15 minutes. Next, it is tested in yellow nematode sista in potatoes plants in the greenhouse. The result of this research shows that endofit bacteria filtrate can obstruct the growth of sista Globodera rostochiensis population. Three from those six isolates which have high ability in pressing sista Globodera rostochiensis population in 100 gram of soil are isolate AA (91%), DH (81%) and BE (81%). Isolate AA can press sista Globodera rostochiensis population in the high scale, which reach until 91%. All endofit bacteria isolate (Isolat AA, AH, BA, BE, DA and DH) can increase the growth of potatoes plants, they are the height of plant and the weight of potatoes plants root.
Karakterisasi Enzim Amilase dari Bakteri Bacillus megaterium pada Variasi Suhu, pH dan Konsentrasi Substrat Dina Istia'nah; Ulfah Utami; Ahmad Barizi
Jurnal Riset Biologi dan Aplikasinya Vol. 2 No. 1 (2020)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v2n1.p11-17

Abstract

Bacillus megaterium merupakan salah satu mikroorganisme potensial yang dapat menghasilkan enzim amilase. Karakterisasi enzim amilase dapat membantu mengetahui kondisi optimum enzim saat bekerja. Penelitian ini bertujuan untuk mengetahui karakteristik enzim amilase yang dihasilkan oleh Bacillus megaterium pada variasi suhu, pH dan konsentrasi substrat. Karakterisasi enzim ditentukan dengan menguji aktivitas enzim pada 3 variasi yakni variasi suhu dengan menggunakan pH netral, variasi pH yang dilakukan pada kondisi suhu optimum hasil perlakuan sebelumnya dan variasi konsentrasi substrat YPSs yang dilakukan pada kondisi suhu dan pH optimum hasil perlakuan sebelumnya. Data aktivitas enzim amilase dianalisis secara deskriptif dengan menentukan nilai aktivitas enzim amilase tertinggi pada setiap perlakuan. Hasil penelitian ini menunjukkan bahwa Bacillus megaterium mampu menghasilkan enzim amilase. Indeks Aktivitas Amilase (IAA) Bacillus megaterium sebesar 2,35 mm. Secara kuantitatif aktivitas enzim amilase ditentukan dengan metode DNS yang untuk mengukur kadar gula reduksi yang diproduksi oleh mikrob dengan tingkat ketelitian yang tinggi. Ekstrak kasar enzim amilase yang diproduksi oleh Bacillus megaterium memiliki karakteristik pada suhu 37oC dengan aktivitas enzim sebesar 1,279 U/mL, sedangkan karakteristik pH berada pada pH 5,0 dengan aktivitas enzim sebesar 1,241 U/mL dan karakteristik konsentrasi substrat berada pada konsentrasi 1,50% dengan aktivitas enzim sebesar 0,548 U/mL.
Uji Antagonisme Beberapa Fungi Endofit pada Tanaman Kentang terhadap Fusarium oxysporum secara In Vitro Izzatinnisa' Izzatinnisa'; Ulfah Utami; Ahmad Mujahidin
Jurnal Riset Biologi dan Aplikasinya Vol. 2 No. 1 (2020)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v2n1.p18-25

Abstract

Pemanfaatan fungi endofit merupakan salah satu alternatif untuk mengendalikan Fusarium oxysporum pada tanaman kentang (Solanum tuberosum L.) tanpa menimbulkan dampak negatif terhadap lingkungan. Penelitian ini bertujuan untuk mengetahui daya antagonisme fungi endofit hasil isolasi dari tanaman kentang terhadap F. oxysporum secara in vitro. Penelitian ini bersifat deskriptif eksploratif dan eksperimen. Fungi endofit dan F. oxysporum diisolasi dengan metode direct platting, selanjutnya dilakukan pemurnian dan identifikasi fungi. Fungi endofit yang terpilih, dilakukan uji antagonisme terhadap F. oxysporum secara in vitro dengan metode dual culture. Persentase hambatan dianalisis menggunakan uji ANOVA dan uji lanjut Tukey. Hasil penelitian menunjukkan empat isolat fungi endofit berhasil diisolasi dari jaringan daun tanaman kentang yaitu Mucor sp.1, Mucor sp.2, Neoscytalidium sp., dan Aspergillus sp. Fungi patogen hasil isolasi yaitu F. oxysporum. Uji antagonisme dengan metode dual culture menunjukkan semua fungi endofit mampu menghambat pertumbuhan F. oxysporum pada tanaman kentang dengan persentase hambatan yang bervariasi, yaitu Neoscytalidium sp. (73,09%), Mucor sp.2 (70,88%), Mucor sp.1 (59,84%) dan Aspergillus sp. (66,06%). Hasil analisis ANOVA menunjukkan bahwa terdapat pengaruh dari perlakuan yang diberikan (p=0,001). Dengan demikian, fungi endofit hasil isolasi memiliki potensi dalam menghambat fungi patogen tanaman kentang.
Effect of Nitrogen Source on Growth Endophytic Yeast from Salacca edulis Reinw. and Bread Quality Analysis Nuzulul Zahroh; Ulfah Utami; Nur Kusmiyati
Jurnal Biodjati Vol 7, No 1 (2022): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i1.16854

Abstract

east biomass is often used in the fermentation of bread dough. Dough fermentation can be maximized by adding a nitrogen source. This study used yeast isolates from salak pondoh (YIS-3, YIS-4, and YIS-7). The purpose of this study was to determine the effect of nitrogen addition on yeast growth and bread quality. This study used an experimental approach. The results of the growth study showed that all isolates treated with 0.05% urea produced higher biomass and cell counts than those treated with control. The highest biomass was produced by YIS-7, which was 3.81 g/300mL, while the highest number of cells was produced by YIS-3, which was 29.02x106 cells/mL. The percentage of proofing results showed that all yeast isolates treated with 0.05% urea needed a longer time to achieve the highest proofing. However, the volume of bread after baking showed better results than those treated with control. The largest volume of bread produced by YIS-3, was 972.14 cm3. The results of the organoleptic test showed that P<5%, which means that all treatments had a significant effect on the taste, aroma, color, and texture of the bread. Overall, the panelists gave good acceptance of the bread fermented by YIS-3 with 0.05% urea treatment. So it can be concluded that the addition of urea with a concentration of 0.05% in YIS-3 gave the best effect on the yeast growth and bread quality.
Pelatihan Pembuatan Nata de Coco pada PKK di Tasikmadu, Lowokwaru, Kota Malang Ulfah Utami; Liliek Harianie; Nur Kusmiyati; Prilya Dewi Fitriasari
Jurnal Pengabdian Pada Masyarakat Vol 5 No 1 (2020)
Publisher : Universitas Mathla'ul Anwar Banten

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30653/002.202051.268

Abstract

TRAINING ON MAKING NATA DE COCO FOR PKK ORGANISATION IN TASIKMADU, LOWOKWARU, MALANG CITY. One of the functional food products is nata. Nata is a collection of cellulose which has a chewy texture, white, producing gel sheets that float on the liquid surface. Material that is often used as a medium for making nata is coconut water, known as Nata de Coco. Acetobacter xylinum bacteria are added in the process of making nata de coco on steril condition. The process is important to be informed to the community, in particular the PKK organization through community development program. The methods used include lectures, practice and evaluation. Survey of partners showed that 23,8% had known the process of making nata de coco while 76,2% had not known the process, through this training, 68% partners strongly agree nata production is used as a business idea.
PELATIHAN PEMBUATAN PUPUK BOKASI UNTUK MENDUKUNG PERTANIAN ORGANIK DI SMKN 1 WONOSARI MALANG Nur Kusmiyati; Ulfah Utami; Prilya Dewi Fitriasari; Muhammad Asmuni Hasyim; Fitriyah Fitriyah; Azizatur Rahmah
Jurnal Abdimas Bina Bangsa Vol. 3 No. 2 (2022): Jurnal Abdimas Bina Bangsa
Publisher : LPPM Universitas Bina Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46306/jabb.v3i2.250

Abstract

Community service activities regarding training in making bokasi fertilizers were carried out at SMKN 1 Wonosari Malang to be carried out to support organic farming. The purpose of this service activity is to provide knowledge about making bokasi fertilizers in supporting organic farming efforts. These service activities are carried out using several methods consisting of outreach; demonstrations and practices; guidance followed by mentoring; as well as an analysis of the evaluation of the service program. This training was attended by 30 participants consisting of class XII students at SMKN 1 Wonosari, Malang Regency. Based on the results of the questionnaire data analysis, it can be seen that the knowledge of the participants about bokasi fertilizers in an effort to support organic farming has increased, after participating in training activities; participants are able to make bokasi fertilizer; there is a change in the pattern of knowledge related to bokasi fertilizer so that participants can plan to produce bokasi fertilizer. Overall, it can be said that the training program for making bocation fertilizer in an effort to support organic farming is running effectively and is able to change the knowledge and abilities of students at SMKN 1 Wonosari, Malang Regency
Co-Authors Afifah, Zahroul Ahdiansyah, Balqis Shafa' Ahmad Barizi Anggeria Oktavisa Denta Aprilianty, Windy Marsella Arifin, Naufal Aqila Azizah, Dita Nur Azizatur Rahmah Chaerudin, Denden Ridwan Dina Istia'nah ELDARITA, ELDARITA Elfiyatinnufus, Rifqiyah Fitriyah Fitriyah Fitriyani, Annisa Fuzzi Heriyanto, Yonan Husna, Naila Fatihatul Istia'nah, Dina Izzatinnisa' Izzatinnisa' Izzatinnisa', Izzatinnisa' Kamila, Syadza Afifah Laela, Dewi Sodja Laut, Deru Marah Liliek Harianie Lilik Hariani Marah Laut, Deru Megananda Hiranya Putri Muh Amin, dwi aisyah Muhammad Asmuni Hasyim Muhammad Riefki Pratama Mujahidin Ahmad Neneng Nurjanah Nur Kusmiyati Nurnaningsih, Hera Nurul Fatikhah, Nurul Nuzulul Zahroh Praptiwi, Yenni Hendriani Prilya Dewi Fitriasari Retno Setyaningrum Sari, Cici Rafita Siti Fatimah Sri Mulyanti Sulistyantini, Cahyaning Supriyanto, Irwan Widyastuti , Tri Yuni Puspitasari Yuti Malinda