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All Journal AL KAUNIYAH El-Hayah : Jurnal Biologi Biogenesis: Jurnal Ilmiah Biologi Jurnal Teknologi Pertanian Jurnal Biodjati Jurnal Pengabdian Pada Masyarakat INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY PREPOTIF : Jurnal Kesehatan Masyarakat Jurnal Abdimas Bina Bangsa Jurnal Ilmiah Pengabdian Kepada Masyarakat Jurnal Sains dan Teknologi Kesehatan Journal of Research on Community Engagement
Nur Kusmiyati
Universitas Brawijaya
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Identification of endophytic fungi in nutgrass (Cyperus rotundus L.) as solubilizing phosphate and indole-3-acetic acid producers Nur Kusmiyati; Septian Tri Wicaksono; Durrotul Maknuna
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 1 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i1.21216

Abstract

Low phosphate content in the soil causes insufficient plant needs. Meanwhile, endophytic fungi in nutgrass have great potential as a phosphate solvent and produce indole-3-acetic acid (IAA). Therefore, this study aims to determine the solubilizing phosphate and IAA production by the endophytic fungi of nutgrass and identify the isolates based on rDNA-ITS sequences. Endophytic fungi isolates were cultured in 10 ml of Pikovskaya broth media with Ca5(PO4)3 as the inorganic phosphate source. The PCR results were analyzed using 1.5% agarose gel electrophoresis followed by sequence analysis. The isolation and purification results showed five isolates coded URT1, URT2, URT3, URT4, and URT5, while the solubilizing phosphate levels ranged from 54.03-87.83 ppm, with the highest levels found in the URT4 isolate. Furthermore, the IAA levels ranged from 5.58-45.50 ppm, with the highest levels produced by the URT1 isolate. The molecular analysis with rDNA-ITS sequences showed that URT4 had 97.42% similarity to Aspergillus terreus species, while UTR1 had 100% similarity to Fusarium oxysporum species. Based on the results, the endophytic fungi of nutgrass from A. terreus and F. oxysporum species have great potential as biofertilizers due to the high solubilizing phosphate and IAA levels.
Effect of Nitrogen Source on Growth Endophytic Yeast from Salacca edulis Reinw. and Bread Quality Analysis Nuzulul Zahroh; Ulfah Utami; Nur Kusmiyati
Jurnal Biodjati Vol 7, No 1 (2022): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i1.16854

Abstract

east biomass is often used in the fermentation of bread dough. Dough fermentation can be maximized by adding a nitrogen source. This study used yeast isolates from salak pondoh (YIS-3, YIS-4, and YIS-7). The purpose of this study was to determine the effect of nitrogen addition on yeast growth and bread quality. This study used an experimental approach. The results of the growth study showed that all isolates treated with 0.05% urea produced higher biomass and cell counts than those treated with control. The highest biomass was produced by YIS-7, which was 3.81 g/300mL, while the highest number of cells was produced by YIS-3, which was 29.02x106 cells/mL. The percentage of proofing results showed that all yeast isolates treated with 0.05% urea needed a longer time to achieve the highest proofing. However, the volume of bread after baking showed better results than those treated with control. The largest volume of bread produced by YIS-3, was 972.14 cm3. The results of the organoleptic test showed that P<5%, which means that all treatments had a significant effect on the taste, aroma, color, and texture of the bread. Overall, the panelists gave good acceptance of the bread fermented by YIS-3 with 0.05% urea treatment. So it can be concluded that the addition of urea with a concentration of 0.05% in YIS-3 gave the best effect on the yeast growth and bread quality.
Pelatihan Pembuatan Nata de Coco pada PKK di Tasikmadu, Lowokwaru, Kota Malang Ulfah Utami; Liliek Harianie; Nur Kusmiyati; Prilya Dewi Fitriasari
Jurnal Pengabdian Pada Masyarakat Vol 5 No 1 (2020)
Publisher : Universitas Mathla'ul Anwar Banten

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30653/002.202051.268

Abstract

TRAINING ON MAKING NATA DE COCO FOR PKK ORGANISATION IN TASIKMADU, LOWOKWARU, MALANG CITY. One of the functional food products is nata. Nata is a collection of cellulose which has a chewy texture, white, producing gel sheets that float on the liquid surface. Material that is often used as a medium for making nata is coconut water, known as Nata de Coco. Acetobacter xylinum bacteria are added in the process of making nata de coco on steril condition. The process is important to be informed to the community, in particular the PKK organization through community development program. The methods used include lectures, practice and evaluation. Survey of partners showed that 23,8% had known the process of making nata de coco while 76,2% had not known the process, through this training, 68% partners strongly agree nata production is used as a business idea.
Improving the bread quality and endophytic yeast of Salak Pondoh (Salacca edulis Reinw.) growth by addition of phosphate source Yuanita Refa Kusuma; Ulfah Utami; Nur Kusmiyati
Biogenesis: Jurnal Ilmiah Biologi Vol 10 No 1 (2022)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v10i1.27300

Abstract

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Potential Analysis of Cheese Whey as an Alternative Media Growth for Lactobacillus casei Group Nur Kusmiyati; Maria Massora; Septian Tri Wicaksono
El-Hayah Vol 8, No 4 (2022): EL-HAYAH (VOL 8 NO.4 MARET 2022)
Publisher : Program Study of Biology, Science and Technology Faculty, UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v8i4.15800

Abstract

Cheese processing industry throws a lot of whey into the environment that cause pollution. Whey cheese is known to contain lots of organic matter and lactose which is the main energy source for the Lactobacillus genus. This study aims to determine the effect of using cheese whey media on bacterial cell count, total lactic acid, and total sugar in the Lactobacillus casei group consisting of Lactobacillus casei, Lactobacillus paracasei, and Lactobacillus rhamnosus bacteria. The method used in this study was bacterial culture rejuvenated and propagated on MRSB media for 24 hours. As much as 1% of bacterial culture was inoculated into cheese whey medium and incubated at 37 C for 24 hours. Furthermore, observations were made on the number of bacterial cells, total lactic acid, and total sugar in the L. casei group. The results showed that L. paracasei had the highest number of cells (4.862 CFU/ml), total lactic acid (0.264 mg/ml), and total sugar (93.805 mg/ml) and was not significantly different from L. casei and L. rhamnosus. The cheese whey medium was not significantly different from the positive control (MRSB), however, there was a difference in the growth ability of each isolate on the positive control medium. Based on the research, it was shown that cheese whey media could be used as an alternative medium for the growth of the L. casei group.
PELATIHAN PEMBUATAN PUPUK BOKASI UNTUK MENDUKUNG PERTANIAN ORGANIK DI SMKN 1 WONOSARI MALANG Nur Kusmiyati; Ulfah Utami; Prilya Dewi Fitriasari; Muhammad Asmuni Hasyim; Fitriyah Fitriyah; Azizatur Rahmah
Jurnal Abdimas Bina Bangsa Vol. 3 No. 2 (2022): Jurnal Abdimas Bina Bangsa
Publisher : LPPM Universitas Bina Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46306/jabb.v3i2.250

Abstract

Community service activities regarding training in making bokasi fertilizers were carried out at SMKN 1 Wonosari Malang to be carried out to support organic farming. The purpose of this service activity is to provide knowledge about making bokasi fertilizers in supporting organic farming efforts. These service activities are carried out using several methods consisting of outreach; demonstrations and practices; guidance followed by mentoring; as well as an analysis of the evaluation of the service program. This training was attended by 30 participants consisting of class XII students at SMKN 1 Wonosari, Malang Regency. Based on the results of the questionnaire data analysis, it can be seen that the knowledge of the participants about bokasi fertilizers in an effort to support organic farming has increased, after participating in training activities; participants are able to make bokasi fertilizer; there is a change in the pattern of knowledge related to bokasi fertilizer so that participants can plan to produce bokasi fertilizer. Overall, it can be said that the training program for making bocation fertilizer in an effort to support organic farming is running effectively and is able to change the knowledge and abilities of students at SMKN 1 Wonosari, Malang Regency
Analysis of Exopolysaccharides in Lactobacillus casei group Probiotics from Human Breast Milk Nur Kusmiyati; Yuni Puspitasari; Ulfah Utami; Anggeria Oktavisa Denta
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 5 No 1 (2023): Step up to strengthen the laboratory system and prepare for patients care
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v5i1.2872

Abstract

Exopolysaccharides get a lot of attention because they can improve the host immune system. Exopolysaccharide is a polysaccharide that is produced and secreted from microbes outside the cell, usually found on the outside of the bacterial structure. The Lactobacillus casei group from human breast milk is thought to have the ability to produce exopolysaccharides. The purpose of the study was to examine the exopolysaccharide of the L. casei group that was isolated from breast milk. The methods used include the gravimetric, the phenol-sulfuric acid and the Fourier Transform Infra-Red (FTIR). The results showed that the L. casei group could produce exopolysaccharides, and had high exopolysaccharide total sugar content. Lactobacillus paracasei had the highest exopolysaccharide and total sugar content of 3660 mg/L and 80.6%, respectively. The FTIR results of the L. casei group exopolysaccharides showed the presence of hydroxyl functional groups O-H (3425.76-3295.98 cm-1), methyl C-H (2930.86-2856.70 cm-1), carbonyl C=O (1660.11-1647.27 cm-1), C-H (1456.16-1373.44 cm-1) and C-O-C ether (1071.08-1056.82 cm-1) which are specific characters of exopolysaccharides. Since the FTIR profile demonstrates that the L. casei group can produce exopolysaccharides, it has greater potential as a a probiotic.
SOSIALISASI DAN PELATIHAN PEMBUATAN ROTI DENGAN RAGI KHAMIR LOKAL SACCAROMYCES CEREVISAE Kusmiyati, Nur; Kusnadi, Joni; Denta, Anggeria Oktavisa
Jurnal Ilmiah Pengabdian Kepada Masyarakat Vol. 2 No. 3 (2023): JIPAM : Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : STAI Darul Qalam Tangerang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55883/jipam.v2i3.74

Abstract

The Community Development Program (CDP) broadly aims to provide insight, education and skills in the culinary field to improve family welfare, especially in the people of Sitirejo Village, Wagir District, Malang Regency. The knowledge and skills provided to the community are knowledge about utilizing the local yeast Saccharomyces cerevisae to make bread and to process various types of good and nutritious bread. The planned CDP activities will be held in March-August 2023, at the Sitirejo Village Hall. The target of PPM participants is the Sitirejo Village community who are members of the Family Welfare Empowerment Group (PKK) under the auspices of the Community Development Service and the Population Control and Family Planning Village. The methods used include lectures, discussions and questions and answers, as well as the practice of making various breads using the local yeast Saccharomyces cerevisae. The results of the CDP activities were analyzed using descriptive techniques with percentage values. Based on the results of the CDP activities, it was shown that participants gained new knowledge about local yeast. In addition, CDP training participants are also skilled at making various breads using local yeast. CDP activities are expected to provide an overview related to the catering business to CDP training participants
FUNGI ENDOFIT DAUN Artocarpus altilis SEBAGAI ANTIBAKTERI PADA Staphylococcus aureus DAN Escherichia coli Nur Kusmiyati; Nuzulul Nuzulul Furoida Imarotu Zahroh; Liliek Harianie; Siti Fajariyah Novita; Ulfah Utami; Anggeria Oktavisa Denta
Jurnal Teknologi Pertanian Vol. 25 No. 1 (2024)
Publisher : Fakultas Teknologi Pertanian Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtp.2024.025.01.4

Abstract

Beberapa jenis mikroba dapat menyebabkan patologis yang cukup serius bagi manusia ataupun makhluk hidup lainnya, salah satunya adalah Staphylococcus aureus dan Escherichia coli. Senyawa aktif dari daun sukun Artocarpus altilis memiliki aktivitas antimikroba, namun eksploitasi tanaman dapat mengakibatkan ketidakseimbangan ekosistem. Fungi endofit hidup  di dalam sel jaringan tanaman  sehat dan dapat menghasilkan jenis metabolit sekunder yang sama dengan tanaman inangnya. Tujuan penelitian ini adalah untuk mengisolasi dan menyeleksi fungi endofit dari daun A. altilis yang memiliki potensi sebagai agen antibakteri terhadap  S. aureus dan E. coli. Metode yang digunakan yaitu isolasi fungi endofit dari daun A. altilis  dan mengidentifikasinya secara makroskopis dan mikroskopis. Uji aktivitas antibakteri terhadap S. aureus dan E. coli dilakukan ekstraksi metabolit sekunder bakteri endofit dan uji fitokimia menggunakan metode difusi, pengenceran cair, dan padat. Berdasarkan hasil analisis didapatkan empat isolat fungi endofit daun A. altilis yang diberi kode IS01, IS02, IS03 dan IS04. Fungi endofit IS01 memiliki aktivitas antibakteri sangat kuat, IS03 kuat, IS02 sedang, dan IS04 lemah terhadap patogen E. coli dan kuat terhadap patogen S. aureus. Fungi endofit daun A. altilis dapat berperan sebagai  antibakteri terhadap  S. aureus dan E. coli.
Analisis Pertumbuhan Khamir Endofit Buah Salak (Salacca edulis Reinw.) dengan Penambahan Zinc Nur Kusmiyati; Ulfah Utami; Muhammad Riefki Pratama; Liliek Harianie
Al-Kauniyah: Jurnal Biologi Vol 17, No 2 (2024): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v17i2.31921

Abstract

AbstrakKhamir endofit buah salak (Salacca edulis Reinw.) dengan kode YIS-3, YIS-4, dan YIS-7 dapat dimanfaatkan untuk fermentasi adonan roti. Kemampuan tersebut dapat ditingkatkan dengan penambahan sumber zinc pada media pertumbuhannya. Tujuan penelitian ini untuk menganalisis pertumbuhan khamir endofit buah salak dengan penambahan zinc dan kualitas roti hasil fermentasi. Metode yang digunakan meliputi peremajaan isolat khamir, penambahan nutrisi pada media pertumbuhan, analisis biomassa khamir, jumlah sel, volume adonan, dan organoleptik. Hasil penelitian pada parameter pertumbuhan menunjukkan bahwa perlakuan dengan penambahan zinc 0,1 g/L menghasilkan biomassa dan jumlah sel lebih tinggi dibandingkan perlakuan kontrol. Biomassa tertinggi dihasilkan oleh YIS-4 yakni 4,13 g/300 mL, sedangkan jumlah sel tertinggi dihasilkan oleh YIS-3 yakni 27,84 x 106 sel/mL. Pada persentase pengembangan menunjukkan bahwa isolat khamir dengan perlakuan zinc 0,1 g/L membutuhkan waktu lebih lama untuk mencapai pengembangan tertinggi, namun volume roti setelah pemanggangan menunjukkan hasil yang lebih baik dibanding perlakuan kontrol. Isolat YIS-4 dengan perlakuan zinc 0,1 g/L menghasilkan volume roti tertinggi (949,54 cm3). Berdasarkan analisis organoleptik dengan parameter warna, aroma, rasa dan tekstur menunjukkan bahwa semua panelis lebih menyukai roti hasil fermentasi isolat YIS-4 dengan perlakuan zinc 0,1 g/L.AbstractThe endophytic yeast of salak fruit (Salacca edulis Reinw.) with codes YIS-3, YIS-4, and YIS-7 can be used for bread dough fermentation. This ability can be increased by adding a zinc source to the growth medium. The purpose of this study was to analyze the growth of the endophytic yeast of salak fruit with the addition of zinc and the quality of fermented bread. The methods used include rejuvenation of yeast isolates, addition of nutrients to growth media, analysis of yeast biomass, cell count, dough volume and organoleptic. The results of research on growth parameters showed that treatment with the addition of zinc 0.1 g/L resulted in higher biomass and cell count than the control treatment. The highest biomass was produced by YIS-4 which was 4.13 g/300 mL, while the highest number of cells was produced by YIS-3 which was 27.84 x 106 cells/mL. The swelling percentage showed that yeast isolates treated with 0.1 g/L zinc took longer to reach the highest swelling, but the volume of bread after baking showed better results than the control treatment. YIS-4 isolate treated with 0.1 g/L zinc produced the highest bread volume (949.54 cm3). Based on organoleptic analysis with parameters of color, aroma, taste and texture, it showed that all panelists preferred bread fermented YIS-4 isolate with 0.1 g/L zinc treatment.
Co-Authors Achmad Fauzan, Achmad Anggeria Oktavisa Denta Anggeria Oktavisa Denta Anik Listiyana Azizatur Rahmah Denta, Anggeria Denta, Anggeria Oktavisa Durrotul Maknuna Elok Zubaidah Fauziyah S, Endang Fitriyah Fitriyah Hasfiani, Yuliatin Hidayat Sujuti Joni Kusnadi Liliek Harianie Maria Massora Mentari Sekar Arum Muhammad Asmuni Hasyim Muhammad Riefki Pratama Nuzulul Nuzulul Furoida Imarotu Zahroh Nuzulul Zahroh Pratama, Muhammad Riefki Prilya Dewi Fitriasari Sari, Cici Rafita Septian Tri Wicaksono Septian Tri Wicaksono Siti Fajariyah Novita Ulfah Utami Ulfah Utami Watiningrum, Rahayu Yuliana Wicaksono, Septian Tri Yuanita Refa Kusuma Yuliana Watiningrum, Rahayu Yuni Puspitasari Zahroh, Nuzulul Furoida Imarotu