The Effect of Storage Conditions on the Absorption Profile of 2% Lydocaine Hydrochloridum Injection by UV-Vis Spectrophotometry

Dian Marlina (Universitas Setia Budi (USB), Surakarta)
Sephia Febriyanti (Universitas Setia Budi, Surakarta)
Arsyeni Destra Anarchy Arsyandi (Universitas Setia Budi, Surakarta)
Nur Anggreini Dwi Sasangka (Universitas Setia Budi, Surakarta)

Advance Sustainable Science, Engineering and Technology (ASSET)
Vol 5, No 1 (2023): November-April

Lidocaine HCl injection is a sterile product that is easily contaminated when opened. The temperature and humidity in the injection storage conditions also affect the quality of the drug when viewed from the UV-Vis spectrophotometric absorption value. The aimed of this study is to find out how the effect of storage conditions on the absorption profile of 2% lydocaine HCl injection by UV-Vis spectrophotometry. The study used 27 ampoules of 2% lidocaine HCl injection and was qualitatively analyzed using UV-Vis spectrophotometry. The study lasted for 14 days by first storing the injections in storage conditions at room temperature (25-27℃), cold temperatures (5-10℃), and exposed to Ultraviolet light (380 nm). Based on the results of laboratory tests, the most suitable storage condition used for storing lidocaine HCl injection was cold storage (5-10℃) because there was no shift in the maximum wavelength as occurred during the 14th day of UV exposure storage.